Abstract
To probe the molecular mechanisms of plant resistance to heavy metals,a clone of PvSR4 was isolated from a mercuric chloride treated bean( Phaseolus vulgaris L.)cDNA library by differential screening.The sequences of the cDNA insert and protein homological analysis showed that PvSR4 encoded an intracellular pathogenesis related protein,and the purified protein showed ribonuclease activity.Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid,wounding also stimulated the synthesis of PvSR4 mRNAs,whereas elevated temperature had almost no effect.These suggested that PvSR4 protein might play important roles in resistance to heavy metal stress.
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Claims 8
Claim · model-written summary
PvSR4 encoded an intracellular pathogenesis-related protein in Phaseolus vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Gene
- PvSR4e1
- Outcome
- intracellular pathogenesis related proteine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a clone of PvSR4 was isolated from a mercuric chloride treated bean( Phaseolus vulgaris L.)cDNA library by differential screening”
e1“PvSR4 encoded an intracellular pathogenesis related protein”
Claim · model-written summary
The purified PvSR4 protein showed ribonuclease activity.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Gene
- the purified proteine2
- Outcome
- ribonuclease activitye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a clone of PvSR4 was isolated from a mercuric chloride treated bean( Phaseolus vulgaris L.)cDNA library by differential screening”
e2“the purified protein showed ribonuclease activity”
Claim · model-written summary
PvSR4 transcripts were undetectable in unstressed bean leaves.
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
Claim · model-written summary
PvSR4 gene expression in bean leaves was strongly induced by heavy metals relative to unstressed leaves.
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
Claim · model-written summary
PvSR4 gene expression in bean leaves was induced by salicylic acid relative to unstressed leaves.
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
Claim · model-written summary
Wounding stimulated the synthesis of PvSR4 mRNAs in bean.
Not reportedComparator
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
e4“wounding also stimulated the synthesis of PvSR4 mRNAs,whereas elevated temperature had almost no effect”
Claim · model-written summary
Elevated temperature had almost no effect on PvSR4 gene expression in bean.
- Taxon
- beane3
- Treatment
- elevated temperaturee4
- Gene
- PvSR4e4
- Outcome
- gene expressione3
- Direction
- no_changeinferrede4
Not reportedComparator
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
e4“wounding also stimulated the synthesis of PvSR4 mRNAs,whereas elevated temperature had almost no effect”
Claim · model-written summary
The authors suggest that PvSR4 protein may play important roles in bean resistance to heavy metal stress.
Verbatim
e3“Northern blot analysis revealed that the PvSR4 transcripts were undetectable in unstressed bean leaves,but the gene expression was strongly induced by heavy metals and salicylic acid”
e5“These suggested that PvSR4 protein might play important roles in resistance to heavy metal stress”
The same questions in other papers
salicylic acid · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →mechanical · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- no effect
heat · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
2 in the opposite direction
- positive
- positive
heavy metal · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.