Abstract
Nucleotides are the basic elements of the genetic material, participate in bio-energetic processes, are cofactors and components of secondary metabolites, etc. Nucleotide hydrolases (nucleotidases) are phosphatases that remove the 5'-phosphate group from the nucleotides and play a crucial role in nucleotide metabolism. In this study, genes encoding putative nucleotidases in Phaseolus vulgaris have been identified, and the effect of methyl jasmonate (MeJA) on both nucleotidase activity and gene expression has been addressed. The predicted nucleotidase peptides include the conserved domains characteristic of the haloacid dehalogenase-like hydrolase superfamily. The analysis of the expression of the 11 identified genes in radicles of common bean seedlings elicited with MeJA showed that 3 of them are highly induced by this phytohormone in a dose-dependent manner. Nucleotidase activity in radicles from MeJA treated plants was higher than in not elicited seedlings, and this induction was observed with all the nucleotides assayed (mono-, di- or triphosphate) and with purine or pyrimidine nucleotides. MeJA is involved in biotic and abiotic stress, and the induction of nucleotide metabolism in response to this treatment suggests a relevant role for nucleotides in the seedlings response to unfavourable conditions.
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Claims 5
Claim · model-written summary
Genes encoding putative nucleotidases were identified in Phaseolus vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Gene
- genes encoding putative nucleotidasese0
- Outcome
- have been identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes encoding putative nucleotidases in Phaseolus vulgaris have been identified”
Claim · model-written summary
Predicted nucleotidase peptides contained conserved haloacid dehalogenase-like hydrolase domains.
- Taxon
- common beanPhaseolus vulgarist0
- Gene
- nucleotidase peptidese0
- Outcome
- conserved domains characteristic of the haloacid dehalogenase-like hydrolase superfamilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted nucleotidase peptides include the conserved domains characteristic of the haloacid dehalogenase-like hydrolase superfamily”
t0“common bean seedlings”
Claim · model-written summary
Three of 11 nucleotidase genes were highly induced dose dependently by methyl jasmonate in bean radicles.
- Taxon
- common bean seedlingsPhaseolus vulgarise0
- Treatment
- MeJAe0
- Outcome
- genes in radiclese0
- Direction
- highly inducede0
- Value
- 3 of theme0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 11 identified genes in radicles of common bean seedlings elicited with MeJA showed that 3 of them are highly induced by this phytohormone in a dose-dependent manner”
Claim · model-written summary
Methyl jasmonate raised nucleotidase activity in bean radicles relative to untreated seedlings across assayed nucleotides.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- MeJAe0
- Outcome
- Nucleotidase activity in radiclese0
- Direction
- highere0
- Qualifier
- all the nucleotides assayed (mono-, di- or triphosphate) and with purine or pyrimidine nucleotidese0
- Comparator
- not elicited seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nucleotidase activity in radicles from MeJA treated plants was higher than in not elicited seedlings, and this induction was observed with all the nucleotides assayed (mono-, di- or triphosphate) and with purine or pyrimidine nucleotides”
t0“common bean seedlings”
Claim · model-written summary
The authors suggest nucleotide metabolism may contribute to bean seedling responses to unfavorable conditions.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- seedlings response to unfavourable conditionse0
- Qualifier
- induction of nucleotide metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the induction of nucleotide metabolism in response to this treatment suggests a relevant role for nucleotides in the seedlings response to unfavourable conditions”
t0“common bean seedlings”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →(-)-methyl jasmonate · physiology: enzyme activity · Phaseolus vulgaris (common bean)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.