Abstract
The Arabidopsis thaliana transcription factor DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2A (DREB2A) controls the expression of many genes involved in the plant's response to dehydration and heat stress. Despite the significance of post-translational regulation in DREB2A activation, the mechanism underlying this activation remains unclear. Here, with the aid of a newly produced antibody against DREB2A, we characterized the regulation of DREB2A stability in plants exposed to stress stimuli. Endogenous DREB2A accumulated in wild-type Arabidopsis plants subjected to dehydration and heat stress. A degradation assay using Arabidopsis T87 suspension-cultured cells revealed that DREB2A protein degradation was inhibited at high temperatures. The proteasome-dependent degradation of DREB2A required the import of this protein into the nucleus. The E3 ligases DRIP1 and DRIP2 were involved in this process under both normal and stressful conditions; however, other E3 ligases may have also been involved, at least during the late stages of the heat stress response. Although the constitutive expression of DREB2A resulted in an overproduction of DREB2A and enhanced target gene induction during stress in transgenic plants, the accumulation of DREB2A caused by proteasome inhibitors did not induce target gene expression. Thus, the stabilization of DREB2A is important but not sufficient to induce target gene expression; further activation processes are required.
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Claims 10
Claim · model-written summary
Endogenous DREB2A accumulated under dehydration stress.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- dehydratione0
- Gene
- DREB2Ae0
- Outcome
- Endogenous DREB2Ae0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endogenous DREB2A accumulated in wild-type Arabidopsis plants subjected to dehydration and heat stress.”
Claim · model-written summary
Endogenous DREB2A accumulated under heat stress.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- heat stresse0
- Gene
- DREB2Ae0
- Outcome
- Endogenous DREB2Ae0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endogenous DREB2A accumulated in wild-type Arabidopsis plants subjected to dehydration and heat stress.”
Claim · model-written summary
High temperature inhibited DREB2A protein degradation.
- Taxon
- ArabidopsisArabidopsise0
- Material
- T87 suspension-cultured cellse0
- Treatment
- high temperaturese0
- Gene
- DREB2Ae0
- Outcome
- DREB2A protein degradatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A degradation assay using Arabidopsis T87 suspension-cultured cells revealed that DREB2A protein degradation was inhibited at high temperatures.”
Claim · model-written summary
Proteasome-dependent DREB2A degradation required nuclear import of the protein.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DREB2Ae0
- Outcome
- proteasome-dependent degradation of DREB2Ae0
- Qualifier
- import of this protein into the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proteasome-dependent degradation of DREB2A required the import of this protein into the nucleus.”
t0“Arabidopsis”
Claim · model-written summary
DRIP1 and DRIP2 participated in DREB2A degradation under normal and stressful conditions.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DRIP1 and DRIP2e0
- Outcome
- this processe0
- Qualifier
- under both normal and stressful conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The E3 ligases DRIP1 and DRIP2 were involved in this process under both normal and stressful conditions”
t0“Arabidopsis”
Claim · model-written summary
Other E3 ligases may also participate late in the heat response.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- other E3 ligasese0
- Outcome
- heat stress responsee0
- Qualifier
- late stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other E3 ligases may have also been involved, at least during the late stages of the heat stress response.”
t0“Arabidopsis”
Claim · model-written summary
Constitutive DREB2A expression overproduced DREB2A in transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- constitutive expression of DREB2Ae0
- Gene
- DREB2Ae0
- Outcome
- overproduction of DREB2Ae0
- Direction
- overproductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the constitutive expression of DREB2A resulted in an overproduction of DREB2A and enhanced target gene induction during stress in transgenic plants”
t0“Arabidopsis”
Claim · model-written summary
Constitutive DREB2A expression enhanced target gene induction during stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- constitutive expression of DREB2Ae0
- Gene
- DREB2Ae0
- Outcome
- target gene inductione0
- Direction
- enhancede0
- Qualifier
- during stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the constitutive expression of DREB2A resulted in an overproduction of DREB2A and enhanced target gene induction during stress in transgenic plants”
t0“Arabidopsis”
Claim · model-written summary
Proteasome inhibitors accumulated DREB2A without inducing target gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- proteasome inhibitorse0
- Gene
- DREB2Ae0
- Outcome
- target gene expressione0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of DREB2A caused by proteasome inhibitors did not induce target gene expression.”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that DREB2A stabilization alone is insufficient for target gene induction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DREB2Ae0
- Outcome
- target gene expressione0
- Qualifier
- further activation processes are requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stabilization of DREB2A is important but not sufficient to induce target gene expression; further activation processes are required.”
t0“Arabidopsis”
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