Abstract
So far little is known on the functional role of phosphorylation in the heat stress response of plants. Here we present evidence that heat stress activates the Arabidopsis mitogen-activated protein kinase MPK6. In vitro and in vivo evidence is provided that MPK6 specifically targets the major heat stress transcription factor HsfA2. Activation of MPK6 results in complex formation with HsfA2. MPK6 phosphorylates HsfA2 on T249 and changes its intracellular localisation. Protein kinase and phosphatase inhibitor studies indicate that HsfA2 protein stability is regulated in a phosphorylation-dependent manner, but this mechanism is independent of MPK6. Overall, our data show that heat stress-induced targeting of HsfA2 by MPK6 participates in the complex regulatory mechanism how plants respond to heat stress.
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Claims 7
Claim · model-written summary
Heat stress activates Arabidopsis MPK6.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- heat stresse0
- Gene
- MPK6e0
- Outcome
- mitogen-activated protein kinase MPK6e0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heat stress activates the Arabidopsis mitogen-activated protein kinase MPK6.”
Claim · model-written summary
MPK6 specifically targets HsfA2 in vitro and in vivo.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MPK6e0
- Outcome
- specifically targets the major heat stress transcription factor HsfA2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro and in vivo evidence is provided that MPK6 specifically targets the major heat stress transcription factor HsfA2.”
t0“Arabidopsis”
Claim · model-written summary
MPK6 activation results in complex formation with HsfA2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MPK6e0
- Outcome
- complex formation with HsfA2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activation of MPK6 results in complex formation with HsfA2.”
t0“Arabidopsis”
Claim · model-written summary
MPK6 phosphorylates HsfA2 on T249.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HsfA2e0
- Outcome
- phosphorylates HsfA2 on T249e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MPK6 phosphorylates HsfA2 on T249 and changes its intracellular localisation.”
t0“Arabidopsis”
Claim · model-written summary
MPK6 changes HsfA2 intracellular localisation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HsfA2e0
- Outcome
- intracellular localisatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MPK6 phosphorylates HsfA2 on T249 and changes its intracellular localisation.”
t0“Arabidopsis”
Claim · model-written summary
HsfA2 stability is regulated by phosphorylation independently of MPK6.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HsfA2e0
- Outcome
- HsfA2 protein stabilitye0
- Qualifier
- independent of MPK6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HsfA2 protein stability is regulated in a phosphorylation-dependent manner, but this mechanism is independent of MPK6.”
t0“Arabidopsis”
Claim · model-written summary
Heat-stress-induced MPK6 targeting of HsfA2 participates in regulation of plant heat response.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HsfA2e0
- Outcome
- plants respond to heat stresse0
- Qualifier
- heat stress-induced targeting of HsfA2 by MPK6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heat stress-induced targeting of HsfA2 by MPK6 participates in the complex regulatory mechanism how plants respond to heat stress.”
t0“Arabidopsis”
The same questions in other papers
heat · quality: protein · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.