Abstract
Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttranslational, are important for the activation of gene expression by DREB2A in Arabidopsis (Arabidopsis thaliana). However, the regulatory system of DREB2 in soybean is not clear. We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature. GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization. Despite the partial similarity between these sequences, the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts, suggestive of a conserved regulatory mechanism that involves the recognition of serine/threonine-rich sequences with a specific pattern. The heterologous expression of GmDREB2A;2 in Arabidopsis induced DRE-regulated stress-inducible genes and improved stress tolerance. However, there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A. Therefore, the basic function and regulatory machinery of DREB2 have been maintained between Arabidopsis and soybean, although differentiation has also occurred.
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Claims 8
Claim · model-written summary
GmDREB2A;2 was induced by dehydration, heat and low temperature.
- Taxon
- soybean
Glycine maxe1 - Treatment
- dehydration and heat but also by low temperaturee0
- Gene
- GmDREB2A;2e0
- Outcome
- highly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature.”
e1“Soybean (Glycine max)”
Claim · model-written summary
GmDREB2A;2 showed high transactivation activity via DRE.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmDREB2A;2e0
- Outcome
- transactivation activitye0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region”
e1“Soybean (Glycine max)”
Claim · model-written summary
GmDREB2A;2 has a serine/threonine-rich region corresponding to a negative regulatory domain.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmDREB2A;2e0
- Outcome
- serine/threonine-rich regione0
- Qualifier
- negative regulatory domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization.”
e1“Soybean (Glycine max)”
Claim · model-written summary
Removing the serine/threonine-rich region enhanced GmDREB2A;2 activity in Arabidopsis and soybean protoplasts.
- Taxon
- Arabidopsis and soybean
ArabidopsisGlycine maxe0 - Treatment
- removal of the serine/threonine-rich regione0
- Gene
- GmDREB2A;2e0
- Outcome
- activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts”
Claim · model-written summary
Removing the region enhanced GmDREB2A;2 stability in Arabidopsis and soybean protoplasts.
- Taxon
- Arabidopsis and soybeanArabidopsisGlycine maxe0
- Treatment
- removal of the serine/threonine-rich regione0
- Gene
- GmDREB2A;2e0
- Outcome
- stabilitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts”
Claim · model-written summary
Heterologous GmDREB2A;2 expression induced DRE-regulated stress-inducible genes in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- heterologous expression of GmDREB2A;2e0
- Gene
- GmDREB2A;2e0
- Outcome
- DRE-regulated stress-inducible genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterologous expression of GmDREB2A;2 in Arabidopsis induced DRE-regulated stress-inducible genes and improved stress tolerance.”
Claim · model-written summary
Heterologous GmDREB2A;2 expression improved stress tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- heterologous expression of GmDREB2A;2e0
- Gene
- GmDREB2A;2e0
- Outcome
- stress tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterologous expression of GmDREB2A;2 in Arabidopsis induced DRE-regulated stress-inducible genes and improved stress tolerance.”
Claim · model-written summary
GmDREB2A;2 and DREB2A transgenics differed in growth phenotypes, induced genes and induction ratios.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- GmDREB2A;2e0
- Outcome
- growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratiose0
- Comparator
- DREB2Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A.”
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