Abstract
Post-transcriptional gene silencing (PTGS) is a sequence-specific RNA degradation process conserved in fungi, plants and animals. The trigger of the mechanism is double-stranded RNA derived from transgenic or endogenous loci and formed by intra- or inter-molecular interactions of single-stranded RNAs or the action of RNA-dependent RNA polymerases (RDRs). Double-stranded RNA from various sources is processed by one of the four Dicer-like (DCL) proteins in Arabidopsis, and the resulting short RNAs enter into at least four different pathways, one of which involves the production of trans-acting short interfering RNAs (tasiRNAs). We report here a novel gene (SDE5) that is required for transgene silencing and the production of tasiRNAs. Mutation in SDE5 also results in hyper-susceptibility to cucumber mosaic virus but not turnip mosaic virus. However, like RDR6, SDE5 is not involved in inverted repeat-induced transgene silencing or the biogenesis of microRNAs and 24 nt siRNAs produced by DCL3. Based on these results, we propose that SDE5 acts together with RDR6 in generating double-stranded RNA from specific single-stranded RNAs. As the sequence of SDE5 has sequence features shared by TAP, a human mRNA export factor, we propose that its role could be in the transport of RNA molecules that are converted into a double-stranded form by RDR6.
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Claims 8
Claim · model-written summary
SDE5 is required for transgene silencing.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- SDE5e0
- Outcome
- transgene silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel gene (SDE5) that is required for transgene silencing and the production of tasiRNAs”
e1“in Arabidopsis”
Claim · model-written summary
SDE5 is required for tasiRNA production.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- production of tasiRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel gene (SDE5) that is required for transgene silencing and the production of tasiRNAs”
e1“in Arabidopsis”
Claim · model-written summary
SDE5 mutation increased susceptibility to cucumber mosaic virus.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Mutation in SDE5e0
- Interactor
- cucumber mosaic virus
cucumber mosaic cucumoviruse0pathogen - Gene
- SDE5e0
- Outcome
- hyper-susceptibility to cucumber mosaic viruse0
- Direction
- hyper-susceptibilitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in SDE5 also results in hyper-susceptibility to cucumber mosaic virus but not turnip mosaic virus”
e1“in Arabidopsis”
Claim · model-written summary
SDE5 mutation did not increase susceptibility to turnip mosaic virus.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Mutation in SDE5e0
- Interactor
- turnip mosaic virus
Turnip mosaic viruse0pathogen - Gene
- SDE5e0
- Outcome
- turnip mosaic viruse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in SDE5 also results in hyper-susceptibility to cucumber mosaic virus but not turnip mosaic virus”
e1“in Arabidopsis”
Claim · model-written summary
SDE5 was not involved in inverted-repeat-induced transgene silencing.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- inverted repeat-induced transgene silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 is not involved in inverted repeat-induced transgene silencing or the biogenesis of microRNAs and 24 nt siRNAs produced by DCL3”
e1“in Arabidopsis”
Claim · model-written summary
SDE5 was not involved in biogenesis of microRNAs and DCL3-produced 24 nt siRNAs.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- biogenesis of microRNAs and 24 nt siRNAs produced by DCL3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 is not involved in inverted repeat-induced transgene silencing or the biogenesis of microRNAs and 24 nt siRNAs produced by DCL3”
e1“in Arabidopsis”
Claim · model-written summary
The authors propose that SDE5 works with RDR6 to generate double-stranded RNA from specific single-stranded RNAs.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- generating double-stranded RNA from specific single-stranded RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we propose that SDE5 acts together with RDR6 in generating double-stranded RNA from specific single-stranded RNAs”
e1“in Arabidopsis”
Claim · model-written summary
The authors propose that SDE5 may transport RNA molecules converted to double-stranded RNA by RDR6.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- SDE5e1
- Outcome
- transport of RNA moleculese0
- Qualifier
- converted into a double-stranded form by RDR6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we propose that its role could be in the transport of RNA molecules that are converted into a double-stranded form by RDR6”
e1“sequence of SDE5 has sequence features shared by TAP”
e2“in Arabidopsis”
The same questions in other papers
loss of function · cucumber mosaic cucumovirus · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
loss of function · Turnip mosaic virus · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.