Abstract
Precise coordination between stamen and pistil development is essential to make a fertile flower. Mutations impairing stamen filament elongation, pollen maturation, or anther dehiscence will cause male sterility. Deficiency in plant hormone gibberellin (GA) causes male sterility due to accumulation of DELLA proteins, and GA triggers DELLA degradation to promote stamen development. Deficiency in plant hormone jasmonate (JA) also causes male sterility. However, little is known about the relationship between GA and JA in controlling stamen development. Here, we show that MYB21, MYB24, and MYB57 are GA-dependent stamen-enriched genes. Loss-of-function of two DELLAs RGA and RGL2 restores the expression of these three MYB genes together with restoration of stamen filament growth in GA-deficient plants. Genetic analysis showed that the myb21-t1 myb24-t1 myb57-t1 triple mutant confers a short stamen phenotype leading to male sterility. Further genetic and molecular studies demonstrate that GA suppresses DELLAs to mobilize the expression of the key JA biosynthesis gene DAD1, and this is consistent with the observation that the JA content in the young flower buds of the GA-deficient quadruple mutant ga1-3 gai-t6 rga-t2 rgl1-1 is much lower than that in the WT. We conclude that GA promotes JA biosynthesis to control the expression of MYB21, MYB24, and MYB57. Therefore, we have established a hierarchical relationship between GA and JA in that modulation of JA pathway by GA is one of the prerequisites for GA to regulate the normal stamen development in Arabidopsis.
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Claims 9
Claim · model-written summary
MYB21, MYB24, and MYB57 are GA-dependent stamen-enriched genes.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- MYB21, MYB24, and MYB57e0
- Outcome
- GA-dependent stamen-enriched genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB21, MYB24, and MYB57 are GA-dependent stamen-enriched genes”
t0“Arabidopsis”
Claim · model-written summary
Loss of RGA and RGL2 restores expression of three MYB genes in GA-deficient plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- GA-deficient plantse0
- Treatment
- Loss-of-function of two DELLAs RGA and RGL2e0
- Gene
- RGA and RGL2e0
- Outcome
- expression of these three MYB genese0
- Direction
- restorese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of two DELLAs RGA and RGL2 restores the expression of these three MYB genes together with restoration of stamen filament growth in GA-deficient plants.”
t0“Arabidopsis”
Claim · model-written summary
Loss of RGA and RGL2 restores stamen filament growth in GA-deficient plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- GA-deficient plantse0
- Treatment
- Loss-of-function of two DELLAs RGA and RGL2e0
- Gene
- RGA and RGL2e0
- Outcome
- stamen filament growthe0
- Direction
- restoratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of two DELLAs RGA and RGL2 restores the expression of these three MYB genes together with restoration of stamen filament growth in GA-deficient plants.”
t0“Arabidopsis”
Claim · model-written summary
The myb21-t1 myb24-t1 myb57-t1 triple mutant has short stamens.
- Taxon
- ArabidopsisArabidopsist0
- Material
- myb21-t1 myb24-t1 myb57-t1 triple mutante0
- Outcome
- short stamen phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the myb21-t1 myb24-t1 myb57-t1 triple mutant confers a short stamen phenotype leading to male sterility”
t0“Arabidopsis”
Claim · model-written summary
The triple mutant is male sterile.
- Taxon
- ArabidopsisArabidopsist0
- Material
- myb21-t1 myb24-t1 myb57-t1 triple mutante0
- Outcome
- male sterilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the myb21-t1 myb24-t1 myb57-t1 triple mutant confers a short stamen phenotype leading to male sterility”
t0“Arabidopsis”
Claim · model-written summary
GA suppression of DELLAs mobilizes DAD1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DAD1e0
- Outcome
- expression of the key JA biosynthesis gene DAD1e0
- Qualifier
- GA suppresses DELLAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA suppresses DELLAs to mobilize the expression of the key JA biosynthesis gene DAD1”
t0“Arabidopsis”
Claim · model-written summary
JA content in young flower buds of the GA-deficient quadruple mutant is much lower than in WT.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ga1-3 gai-t6 rga-t2 rgl1-1e0
- Organ / stage
- young flower budse0
- Outcome
- JA contente0
- Direction
- much lowere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA content in the young flower buds of the GA-deficient quadruple mutant ga1-3 gai-t6 rga-t2 rgl1-1 is much lower than that in the WT”
t0“Arabidopsis”
Claim · model-written summary
GA promotes JA biosynthesis to regulate MYB21, MYB24, and MYB57 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB21, MYB24, and MYB57e0
- Outcome
- expression of MYB21, MYB24, and MYB57e0
- Qualifier
- GA promotes JA biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA promotes JA biosynthesis to control the expression of MYB21, MYB24, and MYB57”
t0“Arabidopsis”
Claim · model-written summary
Modulation of the JA pathway by GA is a prerequisite for normal stamen development.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- normal stamen developmente0
- Qualifier
- modulation of JA pathway by GAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“modulation of JA pathway by GA is one of the prerequisites for GA to regulate the normal stamen development in Arabidopsis”
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