Abstract
An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv. BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field. Key Words: In vitro propagation; banana; Musa sp.DOI: 10.3329/bjar.v34i2.5799Bangladesh J. Agril. Res. 34(2): 269-278, June 2009
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Claims 4
Claim · model-written summary
Shoot regeneration was highest with 7.5 mgL-1 BAP plus 0.5 mgL-1 NAA.
- Taxon
- bananat0
- Treatment
- 7.5 mgL-1 BAP + 0.5 mgL-1 NAAe0
- Outcome
- number of shoot regeneratione0
- Direction
- Higheste0
Not reportedComparator
Verbatim
t0“banana”
e0“Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI)”
Claim · model-written summary
High BAP and NAA concentrations significantly reduced mean shoot number.
- Taxon
- bananat0
- Treatment
- concentrations of BAP and NAA in the medium was highe0
- Outcome
- mean number of shootse0
- Direction
- reducede0
Not reportedComparator
Verbatim
t0“banana”
e0“The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high”
Claim · model-written summary
Shoots rooted on half strength MS medium with IAA and IBA.
Verbatim
t0“banana”
e0“Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI”
Claim · model-written summary
Transferred plantlets established and were ready for field planting.
Verbatim
t0“banana”
e0“the established plantlets are ready for planting in the field”
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