Abstract
Tissue culturing has become a routine method for propagating plants in high technology laboratories. The cost of production using conventional tissue culture is, however, high for most of the countries in the sub-Saharan Africa. In this study, we evaluated a micropropagating protocol for local banana (Musa spp.) (Muunju landrace) in Kenya asan alternative to reduce the unit cost of tissue culture micropropagation. Matrices were satisfactory and comparable to the gelling agents. Glass beads were, however, the best matrix in shoot multiplication. Use of support matrices, locally available macronutrients, micronutrients, sugar, equipment and facility reduced the cost of consumable materialfor banana tissue culturing by about 94%. Putting into account energy, labour and capital investments, the cost dropped from approximately US $ 1.5 to 1.0 per plantlet. Contamination was not observed when the media and equipment were sterilised using a pressure cooker instead of an autoclave. Use of plastic syringes instead of glass cylinders and micropipettes, to measure volumes reduced the cost of the equipment by 96%. The risk of damage and loss due to breakage was eliminated compared to the use of glassware equipment. Shoots were rooted whenthey were transferred to Murashige and Skoog (MS) medium supplemented with 1 mg l-1 napthaleneacetic acid (NAA) or 1 mg l-1 Anatone. Acclimatised plants were successfully transplanted and established in the field. There is potential for use of locally available low cost resources as alternatives to the conventional costly laboratoryresources.
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Claims 9
Claim · model-written summary
Support matrices were satisfactory and comparable to gelling agents in banana micropropagation.
MusaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“local banana (Musa spp.)”
e1“Matrices were satisfactory and comparable to the gelling agents”
Claim · model-written summary
Glass beads were the best matrix for shoot multiplication.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“local banana (Musa spp.)”
e1“Glass beads were, however, the best matrix in shoot multiplication”
Claim · model-written summary
Use of support matrices and locally available macronutrients, micronutrients, sugar, equipment and facility reduced the cost of consumable material for banana tissue culture by about 94%.
- Taxon
- bananae1
- Treatment
- Use of support matrices, locally available macronutrients, micronutrients, sugar, equipment and facilitye1
- Outcome
- cost of consumable materiale1
- Direction
- reducede1
- Value
- about 94%e1
Not reportedComparator
Verbatim
e1“Use of support matrices, locally available macronutrients, micronutrients, sugar, equipment and facility reduced the cost of consumable materialfor banana tissue culturing by about 94%”
Claim · model-written summary
Including energy, labour and capital investments, the cost dropped from approximately US $ 1.5 to 1.0 per plantlet.
Not reportedTaxonTreatmentComparator
Verbatim
e1“Putting into account energy, labour and capital investments, the cost dropped from approximately US $ 1.5 to 1.0 per plantlet”
Claim · model-written summary
No contamination was observed when media and equipment were sterilised in a pressure cooker instead of an autoclave.
- Treatment
- sterilised using a pressure cookere1
- Outcome
- Contaminatione1
- Direction
- not observede1
- Comparator
- autoclavee1
Not reportedTaxon
Verbatim
e1“Contamination was not observed when the media and equipment were sterilised using a pressure cooker instead of an autoclave”
Claim · model-written summary
Using plastic syringes instead of glass cylinders and micropipettes to measure volumes reduced the equipment cost by 96%.
- Treatment
- Use of plastic syringese1
- Outcome
- cost of the equipmente1
- Direction
- reducede1
- Value
- 96%e1
- Comparator
- glass cylinders and micropipettese1
Not reportedTaxon
Verbatim
e1“Use of plastic syringes instead of glass cylinders and micropipettes, to measure volumes reduced the cost of the equipment by 96%”
Claim · model-written summary
The risk of damage and loss due to breakage was eliminated compared with glassware equipment.
- Outcome
- risk of damage and loss due to breakagee1
- Direction
- eliminatede1
- Comparator
- glassware equipmente1
Not reportedTaxonTreatment
Verbatim
e1“The risk of damage and loss due to breakage was eliminated compared to the use of glassware equipment”
Claim · model-written summary
Shoots rooted when transferred to MS medium supplemented with 1 mg l-1 NAA or 1 mg l-1 Anatone.
- Treatment
- Murashige and Skoog (MS) medium supplemented with 1 mg l-1 napthaleneacetic acid (NAA) or 1 mg l-1 Anatonee1
- Outcome
- Shoots were rootede1
Not reportedTaxonComparatorDirection
Verbatim
e1“Shoots were rooted whenthey were transferred to Murashige and Skoog (MS) medium supplemented with 1 mg l-1 napthaleneacetic acid (NAA) or 1 mg l-1 Anatone”
Claim · model-written summary
Acclimatised plants were successfully transplanted and established in the field.
- Taxon
- plantsViridiplantaee1
- Outcome
- successfully transplanted and establishede1
- Setting
- fielde1
Verbatim
e1“Acclimatised plants were successfully transplanted and established in the field”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.