Paper · 2012

Comparative study of in vitro regeneration efficiency of shoot-tip explants of Pelargonium graveolens and some cultivars of banana (Musa spp.) grown in Rwanda in response to different concentrations of cytokinins

D. R Congo

11
claims
15
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

This study examined the in vitro proliferation efficiency of shoot-tip explants from four cultivars of banana (Musa spp) and one species of Pelargonium graveolens. The explants were cultured on Murashige and Skoog (MS) medium supplemented with growth regulators. The comparative in vitro proliferation efficiency of banana cultivars was in the following order: Bluggoe > Injagi > FHIA-25 > Kamaramasenge, the comparative ratio was 10:7:4:2. The best number of shoot buds proliferation was observed on MS medium supplemented with 100 μM BAP while the quick buds development was observed on culture media with 10 μM BAP. The ANOVA proved that the genotype of explants of banana, the concentration in BAP and their interaction have high significant influence on their in vitro proliferation rate. Bluggoe, Injagi and FHIA-25 were described as subjects favorable for Rwanda’s in vitro propagation program of high-performance plant varieties. P. graveolens required high quantity of hormones and bit complicated disinfection compared to banana. For P. graveolens, the medium MS + 100 mM BAP + 1 mM NAA + 1 mM GA3 was the best for in vitro regeneration while the addition of 10 and 100 mM Kin favored the rooting. The results of this study contributed a lot to the in vitro regeneration, with minimal explants material of banana (Musa spp.) and P. graveolens currently carried out in Rwanda by ISAR and IRS, respectively.

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

Banana cultivars ranked Bluggoe, Injagi, FHIA-25, Kamaramasenge for proliferation at a ratio of 10:7:4:2.

Taxon
bananat0
Outcome
comparative in vitro proliferation efficiencye0
Value
10:7:4:2e0

Verbatim

e0

“The comparative in vitro proliferation efficiency of banana cultivars was in the following order: Bluggoe > Injagi > FHIA-25 > Kamaramasenge, the comparative ratio was 10:7:4:2”

✓ located verbatim in the source · abstract, characters 263–439 · show in text ↑
t0

“banana (Musa spp.)”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 100 μM BAP gave the best shoot bud proliferation.

Taxon
bananat0
Treatment
MS medium supplemented with 100 μM BAPe0
Outcome
number of shoot buds proliferatione0
Direction
beste0

Not reportedComparator

Verbatim

e0

“The best number of shoot buds proliferation was observed on MS medium supplemented with 100 μM BAP”

✓ located verbatim in the source · abstract, characters 441–539 · show in text ↑
t0

“banana (Musa spp.)”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Culture medium with 10 μM BAP gave quick bud development.

Taxon
bananat0
Treatment
culture media with 10 μM BAPe0
Outcome
buds developmente0
Direction
quicke0

Not reportedComparator

Verbatim

e0

“the quick buds development was observed on culture media with 10 μM BAP”

✓ located verbatim in the source · abstract, characters 546–617 · show in text ↑
t0

“banana (Musa spp.)”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Banana explant genotype significantly influenced in vitro proliferation rate.

Taxon
bananae0
Material
genotype of explants of bananae0
Outcome
in vitro proliferation ratee0

Not reportedDirection

Verbatim

e0

“the genotype of explants of banana, the concentration in BAP and their interaction have high significant influence on their in vitro proliferation rate”

✓ located verbatim in the source · abstract, characters 641–792 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

BAP concentration significantly influenced banana in vitro proliferation rate.

Taxon
bananae0
Treatment
concentration in BAPe0
Outcome
in vitro proliferation ratee0

Not reportedDirection

Verbatim

e0

“the genotype of explants of banana, the concentration in BAP and their interaction have high significant influence on their in vitro proliferation rate”

✓ located verbatim in the source · abstract, characters 641–792 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Banana explant genotype and BAP concentration interacted significantly in their influence on proliferation rate.

Taxon
bananae0
Material
genotype of explants of bananae0
Treatment
concentration in BAPe0
Outcome
in vitro proliferation ratee0

Not reportedDirection

Verbatim

e0

“the genotype of explants of banana, the concentration in BAP and their interaction have high significant influence on their in vitro proliferation rate”

✓ located verbatim in the source · abstract, characters 641–792 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Bluggoe, Injagi and FHIA-25 were considered favorable for propagation in Rwanda.

Taxon
bananat0
Material
Bluggoe, Injagi and FHIA-25e0
Outcome
favorable for Rwanda’s in vitro propagation programe0
Setting
Rwandae0

Verbatim

e0

“Bluggoe, Injagi and FHIA-25 were described as subjects favorable for Rwanda’s in vitro propagation program of high-performance plant varieties”

✓ located verbatim in the source · abstract, characters 794–936 · show in text ↑
t0

“banana (Musa spp.)”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

P. graveolens required more hormones than banana.

Taxon
P. graveolensPelargonium graveolense0
Outcome
high quantity of hormonese0
Comparator
bananae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“P. graveolens required high quantity of hormones and bit complicated disinfection compared to banana”

✓ located verbatim in the source · abstract, characters 938–1038 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

P. graveolens required more complicated disinfection than banana.

Taxon
P. graveolensPelargonium graveolense0
Outcome
bit complicated disinfectione0
Comparator
bananae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“P. graveolens required high quantity of hormones and bit complicated disinfection compared to banana”

✓ located verbatim in the source · abstract, characters 938–1038 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS + 100 mM BAP + 1 mM NAA + 1 mM GA3 was best for P. graveolens regeneration.

Taxon
P. graveolensPelargonium graveolense0
Treatment
MS + 100 mM BAP + 1 mM NAA + 1 mM GA3e0
Outcome
in vitro regeneratione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For P. graveolens, the medium MS + 100 mM BAP + 1 mM NAA + 1 mM GA3 was the best for in vitro regeneration”

✓ located verbatim in the source · abstract, characters 1040–1146 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding 10 mM Kin favored P. graveolens rooting.

Taxon
P. graveolensPelargonium graveolense0
Treatment
10 and 100 mM Kine0
Outcome
rootinge0
Direction
favorede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For P. graveolens, the medium MS + 100 mM BAP + 1 mM NAA + 1 mM GA3 was the best for in vitro regeneration while the addition of 10 and 100 mM Kin favored the rooting”

✓ located verbatim in the source · abstract, characters 1040–1206 · show in text ↑

N-benzyladenine · growth · MusaResults differ

57 papers: 51 positive, 6 negative or no effect

50 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. no effect
  3. negative

53 papers more in this group

See all 57 papers →

1-naphthaleneacetic acid · growth · Pelargonium graveolens (rose geranium)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Gibberellic acid · growth · Pelargonium graveolens (rose geranium)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

N-benzyladenine · growth · Pelargonium graveolens (rose geranium)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

in vitro · growth · MusaPositive and negative results

68 papers: 58 positive, 10 negative or no effect

57 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

64 papers more in this group

See all 68 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.