Abstract
Three newly selected superior banana types were used to study the effects of different cytokinins and auxins on shoot multiplication and rooting. Benzylaminopurine ((BAP) (5, 10, 20 and 30 µM)) and thidiazuron ((TDZ) (0.4, 1, 2 and 3 µM)) were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) medium, active charcoal (5 g l -1 ), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation more than their use alone. BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation, and BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation. Charcoal alone was better for rooting than auxin treatments or MS medium alone. In conclusion, supplementation of 2 µM TDZ, and 1 µM IAA or 20 µM BAP and 1 µM IAA on MS medium, followed 5 g l -1 charcoal at the rooting stage were the best combinations for the in vitro propagation of banana types. sitokininlerin IAA ile olan kombinasyonlar›, ba¤›ms›z kullan›mlar›na gore surgun say›s›n› ve uzamas›n› artt›rm›flt›r. BAP'›n 20 µM'›n ve TDZ'nin 1 µM'›n alt›nda kullan›m› surgun coalmas›n› artt›rmam›fl, BAP'nin 20 µM'›n ve TDZ'nin 2 µM'›n uzerinde kullan›m› ise surgun uzamas›n› engellemifltir. Aktif komurun ba¤›ms›z kullan›m›, koklenme ac›s›ndan MS ortam› ve dier oksin uygulamalar›ndan daha baflar›l› bulunmufltur. Sonuc olarak, muz tiplerinin in vitro coalt›lmas›nda MS besi ortam›na 2 µM TDZ ve 1 µM IAA ya da 20 µM BAP ve 1 µM IAA , koklenme aflamas›nda ise MS besi ortam›na 5 g l -1 aktif komur ilavesi en uygun kombinasyonlar olarak belirlenmifltir.
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Claims 7
Claim · model-written summary
TDZ increased shoot proliferation and elongation relative to BAP in all three banana types.
Verbatim
e0“Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types.”
t0“banana”
Claim · model-written summary
Cytokinin with IAA increased shoot proliferation and elongation compared with cytokinin alone.
Verbatim
e0“each cytokinin with IAA increased shoot proliferation and elongation more than their use alone”
t0“banana”
Claim · model-written summary
BAP below 20 µM did not increase shoot proliferation.
Not reportedComparator
Verbatim
e0“BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation”
t0“banana”
Claim · model-written summary
TDZ below 1 µM did not increase shoot proliferation.
Not reportedComparator
Verbatim
e0“BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation”
t0“banana”
Claim · model-written summary
BAP over 20 µM suppressed shoot elongation.
Not reportedComparator
Verbatim
e0“BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation”
t0“banana”
Claim · model-written summary
TDZ over 2 µM suppressed shoot elongation.
Not reportedComparator
Verbatim
e0“BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation”
t0“banana”
Claim · model-written summary
Charcoal alone improved rooting relative to auxin treatments or MS medium alone.
Verbatim
e0“Charcoal alone was better for rooting than auxin treatments or MS medium alone.”
t0“banana”
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