Abstract
AIM: Isolation and characterization of a bacterial isolate (strain FP10) from banana rhizosphere with innate potential as fungal antagonist and microbial adjuvant in micropropagation of banana. METHODS AND RESULTS: Bacterium FP10 was isolated from the banana rhizosphere and identified as Pseudomonas aeruginosa based on phenotypic, biochemical traits and sequence homology of partial 622-bp fragment of 16S ribosomal DNA (rDNA) amplicon, with the ribosomal database sequences. Strain FP10 displayed antibiosis towards fungi causing wilt and root necrosis diseases of banana. Production of plant growth hormone, indole-3-acetic acid (IAA), siderophores and phosphate-solubilizing enzyme in FP10 was determined. Strain FP10 tested negative for hydrogen cyanide, cellulase and pectinase, the deleterious traits for plant growth. Screening of antibiotic genes was carried out by polymerase chain reaction using gene-specific primers. Amplification of a 745-bp DNA fragment confirmed the presence of phlD, which is a key gene involved in the biosynthesis of 2,4-diacetylphloroglucinol (DAPG) in FP10. The antibiotic produced by FP10 was confirmed as DAPG using thin layer chromatography, high performance liquid chromatography and Fourier transform infrared and tested for fungal antibiosis towards banana pathogens. Procedures for encapsulation of banana shoot tips with FP10 are described. CONCLUSIONS: Strain FP10 exhibited broad-spectrum antibiosis towards banana fungi causing wilt and root necrosis. DAPG by FP10 induced bulb formation and lysis of fungal mycelia. Encapsulation of banana shoot tips with FP10 induced higher frequency of germination (plantlet development) than nontreated controls on Murashige and Skoog basal medium. Treatment of banana plants with FP10 enhanced plant height and reduced the vascular discolouration as a result of Fusarium oxysporum f. sp. cubense FOC. SIGNIFICANCE AND IMPACT OF THE STUDY: Because of the innate potential of fungal antibiosis by DAPG antibiotic and production of siderophore, plant-growth-promoting IAA and phosphatase, the strain FP10 can be used as biofertilizer as well as a biocontrol agent.
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Claims 11
Claim · model-written summary
Banana rhizosphere strain FP10 was identified as Pseudomonas aeruginosa.
- Taxon
- bananae0
- Interactor
- Pseudomonas aeruginosa
Pseudomonas aeruginosae0biocontrol_agent - Outcome
- identified as Pseudomonas aeruginosae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacterium FP10 was isolated from the banana rhizosphere and identified as Pseudomonas aeruginosa”
Claim · model-written summary
FP10 showed antibiosis toward fungi causing banana wilt and root necrosis.
Verbatim
e0“Strain FP10 displayed antibiosis towards fungi causing wilt and root necrosis diseases of banana.”
Claim · model-written summary
FP10 produced IAA, siderophores and a phosphate solubilizing enzyme.
Verbatim
e0“Production of plant growth hormone, indole-3-acetic acid (IAA), siderophores and phosphate-solubilizing enzyme in FP10 was determined.”
t0“banana rhizosphere”
Claim · model-written summary
FP10 tested negative for hydrogen cyanide, cellulase and pectinase.
Verbatim
e0“Strain FP10 tested negative for hydrogen cyanide, cellulase and pectinase”
t0“banana rhizosphere”
Claim · model-written summary
A DNA fragment confirmed phlD in FP10.
Verbatim
e0“Amplification of a 745-bp DNA fragment confirmed the presence of phlD, which is a key gene involved in the biosynthesis of 2,4-diacetylphloroglucinol (DAPG) in FP10.”
t0“banana rhizosphere”
Claim · model-written summary
The antibiotic produced by FP10 was confirmed as DAPG.
Verbatim
e0“The antibiotic produced by FP10 was confirmed as DAPG”
t0“banana rhizosphere”
Claim · model-written summary
FP10 DAPG induced fungal bulb formation and mycelial lysis.
- Taxon
- bananat0
- Treatment
- DAPG by FP10e0
- Interactor
- fungale0pathogen
- Second interactor
- FP10e0biocontrol_agent
- Outcome
- bulb formation and lysis of fungal myceliae0
Not reportedComparatorDirection
Verbatim
e0“DAPG by FP10 induced bulb formation and lysis of fungal mycelia.”
t0“banana rhizosphere”
Claim · model-written summary
FP10 encapsulation raised banana shoot tip germination relative to untreated controls.
Verbatim
e0“Encapsulation of banana shoot tips with FP10 induced higher frequency of germination (plantlet development) than nontreated controls on Murashige and Skoog basal medium.”
Claim · model-written summary
FP10 treatment increased banana plant height.
- Taxon
- bananae0
- Treatment
- Treatment of banana plants with FP10e0
- Interactor
- FP10e0biocontrol_agent
- Outcome
- plant heighte0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Treatment of banana plants with FP10 enhanced plant height and reduced the vascular discolouration as a result of Fusarium oxysporum f. sp. cubense FOC.”
Claim · model-written summary
FP10 treatment reduced Fusarium associated vascular discoloration in banana.
- Taxon
- bananae0
- Treatment
- Treatment of banana plants with FP10e0
- Interactor
- Fusarium oxysporum f. sp. cubense FOC
Fusarium oxysporum f. sp. cubensee0pathogen - Second interactor
- FP10e0biocontrol_agent
- Outcome
- vascular discolouratione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of banana plants with FP10 enhanced plant height and reduced the vascular discolouration as a result of Fusarium oxysporum f. sp. cubense FOC.”
Claim · model-written summary
The authors propose FP10 as a biofertilizer and biocontrol agent.
Verbatim
e0“the strain FP10 can be used as biofertilizer as well as a biocontrol agent.”
t0“banana rhizosphere”
The same questions in other papers
in vitro · growth · MusaPositive and negative results
57 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
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See all 68 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.