Abstract
Five cultivars of Musa spp (banana and plantain); ‘Berangan Intan’, ‘Berangan’ (AAA), ‘Rastali’, ‘Nangka’ (AAB) and ‘Baka Baling’ (ABB) were chosen to compare the effect of benzylaminopurine (BAP) and thidiazuron (TDZ) on multiplication efficiency in relation with frequency of abnormal shoot regeneration. Shoot tips ofMusa spp. were cultured on MS medium supplemented with various concentrations (0.0, 11.1, 22.2, 33.3 and 44.4 µM) of BAP and (0.0, 0.5, 2, 5 and 7.5 µM) of TDZ. Increasing BAP above 22.2 µM and 33.3 µM increased the number of shoots in‘Berangan Intan’, ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Baka Baling’ respectively, but above 33.3 µM significantly caused higher gross of abnormal shoot regeneration. TDZ in the media up to 2 µM for ‘Baka Baling’, ‘Nangka’ and ‘Rastali’ and 5 µM for ‘Berangan Intan’ and ‘Berangan’ increased the number of shoots per explant, however TDZ at 5 µM resulted in high number of abnormal shoots. In conclusion BAP at 22.2 µM and TDZ at 2 µM were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production for both banana and plantain. Key words: Abnormality index, cytokinin, micro-propagation, Musa spp., shoot tip.
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Claims 7
Claim · model-written summary
Higher BAP concentrations increased shoot number across the five Musa cultivars.
MusaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing BAP above 22.2 µM and 33.3 µM increased the number of shoots in‘Berangan Intan’, ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Baka Baling’ respectively”
t0“Musa spp.”
Claim · model-written summary
BAP above 33.3 µM increased abnormal shoot regeneration.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“above 33.3 µM significantly caused higher gross of abnormal shoot regeneration”
t0“Musa spp.”
Claim · model-written summary
TDZ up to 2 µM increased shoots per explant in Baka Baling, Nangka and Rastali.
- Material
- ‘Baka Baling’, ‘Nangka’ and ‘Rastali’e0
- Treatment
- TDZ in the media up to 2 µMe0
- Outcome
- number of shoots per explante0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ in the media up to 2 µM for ‘Baka Baling’, ‘Nangka’ and ‘Rastali’ and 5 µM for ‘Berangan Intan’ and ‘Berangan’ increased the number of shoots per explant”
t0“Musa spp.”
Claim · model-written summary
TDZ at 5 µM increased shoots per explant in Berangan Intan and Berangan.
- Material
- ‘Berangan Intan’ and ‘Berangan’e0
- Treatment
- 5 µMe0
- Outcome
- number of shoots per explante0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5 µM for ‘Berangan Intan’ and ‘Berangan’ increased the number of shoots per explant”
t0“Musa spp.”
Claim · model-written summary
TDZ at 5 µM produced many abnormal shoots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ at 5 µM resulted in high number of abnormal shoots”
t0“Musa spp.”
Claim · model-written summary
The authors recommend BAP at 22.2 µM for commercial Musa micropropagation.
- Treatment
- BAP at 22.2 µMe0
- Outcome
- most suitable for commercial micropropagation system with low frequency of abnormal shoot productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP at 22.2 µM and TDZ at 2 µM were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production”
t0“Musa spp.”
Claim · model-written summary
The authors recommend TDZ at 2 µM for commercial Musa micropropagation.
- Treatment
- TDZ at 2 µMe0
- Outcome
- most suitable for commercial micropropagation system with low frequency of abnormal shoot productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP at 22.2 µM and TDZ at 2 µM were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production”
t0“Musa spp.”
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