Abstract
The tobacco anionic peroxidase gene encodes the predominant peroxidase isoenzyme in the aerial portions of tobacco. Three kb of the peroxidase promoter was joined to the coding region of the Escherichia coli beta-glucuronidase gene (GUS), and transiently expressed in tobacco mesophyll protoplasts in the presence or absence of plant growth regulators. Benzyladenine, ethylene, and gibberellic acid did not affect peroxidase gene expression. Abscisic acid slightly inhibited expression at high concentrations. The auxins indole-3-acetic acid (IAA) and naphthaleneacetic acid strongly suppressed peroxidase expression. We observed half maximal suppression at 30 microM IAA. An anti-auxin, p-chlorophenoxyisobutyric acid (PCIB), enhanced expression from the peroxidase promoter above that of untreated controls or restored activity when used in combination with IAA. Sequencing 3 kb of the peroxidase promoter revealed many potential regulatory elements based on sequence homology to previously characterized genes. This includes several consensus transcription factor binding sites found in auxin-regulated promoters. 5' deletions of the peroxidase promoter/GUS fusion revealed several positive and negative regulatory elements. An upstream enhancer element was found between -3146 and -638 from the start of transcription. A strong silencer element was observed between -638 and -220. Removal of this silencer resulted in a truncated promoter (-220) with 100% activity of the full-length promoter (-3146). Inhibition by auxin was observed with all 5' deletions.
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Claims 14
Claim · model-written summary
Benzyladenine did not affect tobacco peroxidase gene expression.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- Benzyladeninee0
- Gene
- peroxidase genee0
- Outcome
- peroxidase gene expressione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Benzyladenine, ethylene, and gibberellic acid did not affect peroxidase gene expression”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Ethylene did not affect tobacco peroxidase gene expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- ethylenee0
- Gene
- peroxidase genee0
- Outcome
- peroxidase gene expressione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Benzyladenine, ethylene, and gibberellic acid did not affect peroxidase gene expression”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Gibberellic acid did not affect tobacco peroxidase gene expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- gibberellic acide0
- Gene
- peroxidase genee0
- Outcome
- peroxidase gene expressione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Benzyladenine, ethylene, and gibberellic acid did not affect peroxidase gene expression”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
High concentrations of abscisic acid slightly inhibited expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Abscisic acide0
- Outcome
- expressione0
- Direction
- inhibitede0
- Qualifier
- at high concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid slightly inhibited expression at high concentrations”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Indole-3-acetic acid strongly suppressed peroxidase expression, with half maximal suppression at 30 microM.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- indole-3-acetic acid (IAA)e0
- Outcome
- peroxidase expressione0
- Direction
- suppressede0
- Value
- 30 microMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxins indole-3-acetic acid (IAA) and naphthaleneacetic acid strongly suppressed peroxidase expression. We observed half maximal suppression at 30 microM IAA”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Naphthaleneacetic acid strongly suppressed peroxidase expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- naphthaleneacetic acide0
- Outcome
- peroxidase expressione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxins indole-3-acetic acid (IAA) and naphthaleneacetic acid strongly suppressed peroxidase expression”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
PCIB enhanced peroxidase promoter expression over untreated controls.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- p-chlorophenoxyisobutyric acid (PCIB)e0
- Outcome
- expression from the peroxidase promotere0
- Direction
- enhancede0
- Comparator
- untreated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An anti-auxin, p-chlorophenoxyisobutyric acid (PCIB), enhanced expression from the peroxidase promoter above that of untreated controls”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
PCIB restored promoter activity when combined with IAA.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- p-chlorophenoxyisobutyric acid (PCIB)e1
- Outcome
- activitye0
- Direction
- restorede0
- Qualifier
- in combination with IAAe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“restored activity when used in combination with IAA”
e1“p-chlorophenoxyisobutyric acid (PCIB)”
e2“tobacco mesophyll protoplasts”
Claim · model-written summary
The sequenced peroxidase promoter contained many potential regulatory elements, including auxin-regulated promoter binding sites.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- peroxidase promotere0
- Outcome
- many potential regulatory elementse0
- Value
- 3 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequencing 3 kb of the peroxidase promoter revealed many potential regulatory elements based on sequence homology to previously characterized genes. This includes several consensus transcription factor binding sites found in auxin-regulated promoters”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Promoter deletions revealed positive and negative regulatory elements.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- peroxidase promoter/GUS fusione0
- Outcome
- several positive and negative regulatory elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5' deletions of the peroxidase promoter/GUS fusion revealed several positive and negative regulatory elements”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
An enhancer was located between -3146 and -638.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- An upstream enhancer elemente0
- Value
- -3146 and -638e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An upstream enhancer element was found between -3146 and -638 from the start of transcription”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
A strong silencer was located between -638 and -220.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- A strong silencer elemente0
- Value
- -638 and -220e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A strong silencer element was observed between -638 and -220”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Removing the silencer yielded a truncated promoter with full length promoter activity.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Removal of this silencere0
- Outcome
- activitye0
- Value
- 100%e0
- Comparator
- full-length promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Removal of this silencer resulted in a truncated promoter (-220) with 100% activity of the full-length promoter (-3146)”
e1“tobacco mesophyll protoplasts”
Claim · model-written summary
Auxin inhibited expression with all 5 prime deletions.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- auxine0
- Outcome
- Inhibitione0
- Direction
- Inhibitione0
- Qualifier
- all 5' deletionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition by auxin was observed with all 5' deletions”
e1“tobacco mesophyll protoplasts”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.