Abstract
Tobacco stress-induced1 (Tsi1) is an ethylene-responsive-element binding protein/APETALA2-type transcription factor that plays an important role in both biotic and abiotic stress signaling pathways. We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae). The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge. Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment. Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment. Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1. Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone. Concurrent with this, the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants. These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.
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Claims 15
Claim · model-written summary
Tsip1 interacted with Tsi1 in vitro and in yeast.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- Tsip1e0
- Outcome
- interacts with Tsi1 in vitro and in yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae).”
t0“Tobacco”
Claim · model-written summary
Salicylic acid increased Tsip1 transcript level in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- salicylic acid (SA)e0
- Gene
- Tsip1e0
- Outcome
- transcript level of Tsip1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge.”
Claim · model-written summary
Ethylene increased Tsip1 transcript level in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ethylenee0
- Gene
- Tsip1e0
- Outcome
- transcript level of Tsip1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge.”
Claim · model-written summary
Gibberellic acid increased Tsip1 transcript level in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- gibberellic acide0
- Gene
- Tsip1e0
- Outcome
- transcript level of Tsip1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge.”
Claim · model-written summary
NaCl increased Tsip1 transcript level in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- NaCle0
- Gene
- Tsip1e0
- Outcome
- transcript level of Tsip1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge.”
Claim · model-written summary
Virus challenge increased Tsip1 transcript level in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- virus challengee0
- Gene
- Tsip1e0
- Outcome
- transcript level of Tsip1e0
- Direction
- increasede0
Not reportedInteractorComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge.”
Claim · model-written summary
Tsip1 was associated with the chloroplast surface.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- Tsip1e0
- Outcome
- physically associated with the chloroplast surfacee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment.”
t0“Tobacco”
Claim · model-written summary
SA treatment caused Tsip1 to dissociate from the chloroplast surface.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- SA treatmente0
- Gene
- Tsip1e0
- Outcome
- dissociated from ite0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment.”
t0“Tobacco”
Claim · model-written summary
After SA treatment, Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- SA treatmente0
- Gene
- Tsip1e0
- Outcome
- colocalized and coimmunoprecipitated with Tsi1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment.”
t0“Tobacco”
Claim · model-written summary
Tsip1 expression increased Tsi1-mediated transcription.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Tsip1 expressione0
- Gene
- Tsip1e0
- Outcome
- Tsi1-mediated transcriptione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1.”
t0“Tobacco”
Claim · model-written summary
Tsip1 compensated for loss of the Tsi1 transcriptional activation domain via direct interaction.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- Tsip1e0
- Outcome
- functionally compensate for loss of the Tsi1 transcriptional activation domaine0
- Qualifier
- direct interaction with Tsi1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1.”
t0“Tobacco”
Claim · model-written summary
Coexpression of Tsi1 and Tsip1 gave stronger pathogen resistance than either alone.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- simultaneously coexpressing Tsi1 and Tsip1e0
- Outcome
- pathogen resistancee0
- Direction
- strongere0
- Comparator
- transgenic plants expressing either Tsi1 or Tsip1 alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone.”
t0“Tobacco”
Claim · model-written summary
Coexpression of Tsi1 and Tsip1 gave stronger salt tolerance than either alone.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- simultaneously coexpressing Tsi1 and Tsip1e0
- Outcome
- salt tolerancee0
- Direction
- strongere0
- Comparator
- transgenic plants expressing either Tsi1 or Tsip1 alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone.”
t0“Tobacco”
Claim · model-written summary
Stress-related genes were cooperatively induced in Tsi1/Tsip1 plants.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- Tsi1/Tsip1 transgenic plantse0
- Outcome
- expression of a subset of stress-related genese0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants.”
t0“Tobacco”
Claim · model-written summary
The authors infer Tsi1 recruits Tsip1 to stress-gene promoters to enhance transcriptional activation.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- Tsi1e0
- Outcome
- potentiate Tsi1-mediated transcriptional activatione0
- Qualifier
- recruits Tsip1 to the promoters of stress-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.”
t0“Tobacco”
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