Abstract
Apoplastic ascorbate oxidase (AO) plays significant roles in plant cell growth. However, the mechanism of underlying the transcriptional regulation of AO in Gossypium hirsutum remains unclear. Here, we obtained a 1,920-bp promoter sequence from the Gossypium hirsutum ascorbate oxidase (GhAO1) gene, and this GhAO1 promoter included a number of known cis-elements. Promoter activity analysis in overexpressing pGhAO1::GFP-GUS tobacco (Nicotiana benthamiana) showed that the GhAO1 promoter exhibited high activity, driving strong reporter gene expression in tobacco trichomes, leaves and roots. Promoter 5'-deletion analysis demonstrated that truncated GhAO1 promoters with serial 5'-end deletions had different GUS activities. A 360-bp fragment was sufficient to activate GUS expression. The P-1040 region had less GUS activity than the P-720 region, suggesting that the 320-bp region from nucleotide -720 to -1040 might include a cis-element acting as a silencer. Interestingly, an auxin-responsive cis-acting element (TGA-element) was uncovered in the promoter. To analyze the function of the TGA-element, tobacco leaves transformed with promoters with different 5' truncations were treated with indole-3-acetic acid (IAA). Tobacco leaves transformed with the promoter regions containing the TGA-element showed significantly increased GUS activity after IAA treatment, implying that the fragment spanning nucleotides -1760 to -1600 (which includes the TGA-element) might be a key component for IAA responsiveness. Analyses of the AO promoter region and AO expression pattern in Gossypium arboreum (Ga, diploid cotton with an AA genome), Gossypium raimondii (Gr, diploid cotton with a DD genome) and Gossypium hirsutum (Gh, tetraploid cotton with an AADD genome) indicated that AO promoter activation and AO transcription were detected together only in D genome/sub-genome (Gr and Gh) cotton. Taken together, these results suggest that the 1,920-bp GhAO1 promoter is a functional sequence with a potential effect on fiber cell development, mediated by TGA-element containing sequences, via the auxin-signaling pathway.
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Claims 8
Claim · model-written summary
A 1,920-bp GhAO1 promoter sequence containing known cis-elements was obtained.
- Taxon
- Gossypium hirsutum
Gossypium hirsutumt0 - Gene
- GhAO1e0
- Outcome
- promoter sequencee0
- Value
- 1,920-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“we obtained a 1,920-bp promoter sequence from the Gossypium hirsutum ascorbate oxidase (GhAO1) gene, and this GhAO1 promoter included a number of known cis-elements”
Claim · model-written summary
The GhAO1 promoter drove strong reporter expression in tobacco trichomes, leaves and roots.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- trichomes, leaves and rootse0
- Gene
- GhAO1e0
- Outcome
- reporter gene expressione0
- Direction
- stronge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“the GhAO1 promoter exhibited high activity, driving strong reporter gene expression in tobacco trichomes, leaves and roots”
Claim · model-written summary
Serial 5-prime truncations of GhAO1 differed in GUS activity.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhAO1e0
- Outcome
- GUS activitiese0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“truncated GhAO1 promoters with serial 5'-end deletions had different GUS activities”
Claim · model-written summary
A 360-bp promoter fragment activated GUS expression.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Outcome
- GUS expressione0
- Value
- 360-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“A 360-bp fragment was sufficient to activate GUS expression.”
Claim · model-written summary
P-1040 had lower GUS activity than P-720.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- P-1040 regione0
- Outcome
- GUS activitye0
- Direction
- lesse0
- Comparator
- P-720 regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“The P-1040 region had less GUS activity than the P-720 region”
Claim · model-written summary
A TGA-element was identified in the promoter.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- TGA-elemente0
- Outcome
- auxin-responsive cis-acting elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“an auxin-responsive cis-acting element (TGA-element) was uncovered in the promoter”
Claim · model-written summary
IAA treatment significantly increased GUS activity in tobacco leaves carrying TGA-element promoter regions.
- Taxon
- TobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- IAA treatmente0
- Gene
- TGA-elemente0
- Outcome
- GUS activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“Tobacco leaves transformed with the promoter regions containing the TGA-element showed significantly increased GUS activity after IAA treatment”
Claim · model-written summary
AO promoter activation and transcription were detected together only in D genome/sub-genome Gr and Gh cotton.
- Taxon
- cottonGossypium hirsutume0
- Material
- D genome/sub-genome (Gr and Gh)e0
- Gene
- AOe0
- Outcome
- AO promoter activation and AO transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gossypium hirsutum”
e0“AO promoter activation and AO transcription were detected together only in D genome/sub-genome (Gr and Gh) cotton”
The same questions in other papers
indole-3-acetic acid · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
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