Abstract
Proteinase inhibitor genes are expressed in solanaceous and leguminous plants following wounding of the foliage by mechanical methods. Previous studies have shown that a cloned proteinase inhibitor II-chloramphenicol acetyl transferase (pin2-CAT) chimeric gene is regulated in a wound-inducible manner in transgenic plants. In this study, we analyzed transgenic plant tissues for expression of the pin2-CAT gene in response to various plant hormones. We found that CAT activity was induced in tobacco (Nicotiana tabacum) callus incubated in the absence of any plant growth regulators. Addition of growth regulators to the medium thus permitted us to measure the effects of these substances on the activity of the pin2-CAT gene construction. Cytokinin (BAP) and ethylene (ethophon) even at low concentrations stimulated the expression of CAT activity by 25 to 50%. Abscisic acid at concentrations up to 4.4 x 10(-5) molar had no effect upon CAT activity, but increasing auxin (naphthalene acetic acid) levels completely inhibited the synthesis of CAT protein. Gibberellic acid had little effect except at very high concentration (2.9 x 10(5) molar). The kinetics of activation of the pin2-CAT gene were quite long (5 to 7 days) when unwounded calli were plated on media lacking auxin. This effect was documented for calli derived from several transformed plants, containing the full, chimeric pin2-CAT (pRT45) gene. In addition, calli from tissues transformed with wild-type vectors or from several plants transformed with pRT50 (a noninducible derivative of pRT45) were not induced by plating on media lacking auxin. Other naturally occurring and synthetic auxins had similar effects to naphthalene acetic acid in inhibiting the induction of the chimeric gene fusion. Finally, leaf discs from transformed plants were induced by incubation in MS liquid medium in the presence and absence of naphthalene acetic acid. NAA was also effective in down regulating the chimeric gene in whole plant tissues.
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Claims 11
Claim · model-written summary
CAT activity was induced in tobacco callus without growth regulators.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume0 - Organ / stage
- calluse0
- Outcome
- CAT activitye0
- Direction
- inducede0
- Qualifier
- absence of any plant growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CAT activity was induced in tobacco (Nicotiana tabacum) callus incubated in the absence of any plant growth regulators.”
Claim · model-written summary
BAP increased CAT activity by 25 to 50%.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Cytokinin (BAP)e0
- Outcome
- CAT activitye0
- Direction
- stimulatede0
- Value
- 25 to 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytokinin (BAP) and ethylene (ethophon) even at low concentrations stimulated the expression of CAT activity by 25 to 50%.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Ethophon increased CAT activity by 25 to 50%.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- ethylene (ethophon)e0
- Outcome
- CAT activitye0
- Direction
- stimulatede0
- Value
- 25 to 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytokinin (BAP) and ethylene (ethophon) even at low concentrations stimulated the expression of CAT activity by 25 to 50%.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
ABA up to 4.4 x 10(-5) molar did not affect CAT activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Abscisic acid at concentrations up to 4.4 x 10(-5) molare0
- Outcome
- CAT activitye0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid at concentrations up to 4.4 x 10(-5) molar had no effect upon CAT activity”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Increasing naphthalene acetic acid completely inhibited CAT synthesis.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- increasing auxin (naphthalene acetic acid) levelse0
- Outcome
- synthesis of CAT proteine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing auxin (naphthalene acetic acid) levels completely inhibited the synthesis of CAT protein.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Gibberellic acid had little effect except at very high concentration.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Gibberellic acide0
- Outcome
- effecte0
- Direction
- little effecte0
- Qualifier
- very high concentration (2.9 x 10(5) molar)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gibberellic acid had little effect except at very high concentration (2.9 x 10(5) molar).”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Activation without auxin took 5 to 7 days.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- pin2-CATe0
- Outcome
- kinetics of activatione0
- Value
- 5 to 7 dayse0
- Qualifier
- media lacking auxine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The kinetics of activation of the pin2-CAT gene were quite long (5 to 7 days) when unwounded calli were plated on media lacking auxin.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Wild-type vector calli did not induce after removal of auxin.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- wild-type vectorse0
- Treatment
- plating on media lacking auxine0
- Outcome
- inducede0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calli from tissues transformed with wild-type vectors or from several plants transformed with pRT50 (a noninducible derivative of pRT45) were not induced by plating on media lacking auxin.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
pRT50 calli did not induce after removal of auxin.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- pRT50e0
- Treatment
- plating on media lacking auxine0
- Outcome
- inducede0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calli from tissues transformed with wild-type vectors or from several plants transformed with pRT50 (a noninducible derivative of pRT45) were not induced by plating on media lacking auxin.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
Other natural and synthetic auxins similarly inhibited transgene induction.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Other naturally occurring and synthetic auxinse0
- Outcome
- induction of the chimeric gene fusione0
- Direction
- inhibitinge0
- Comparator
- naphthalene acetic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other naturally occurring and synthetic auxins had similar effects to naphthalene acetic acid in inhibiting the induction of the chimeric gene fusion.”
t0“in tobacco (Nicotiana tabacum) callus”
Claim · model-written summary
NAA downregulated the chimeric gene in whole plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- whole plant tissuese0
- Treatment
- NAAe0
- Outcome
- the chimeric genee0
- Direction
- down regulatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAA was also effective in down regulating the chimeric gene in whole plant tissues.”
t0“in tobacco (Nicotiana tabacum) callus”
The same questions in other papers
ethene · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
1-naphthaleneacetic acid · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
class · quality: protein · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →class · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.