Abstract
The medium has a profound effect on biocontrol agents, including ability to grow and effectiveness in disease control. In this study, growth and antagonistic efficacy of strains P-5 and P-35 (P. fluorescens), B-3 and B-16 (B. subtilis) were evaluated in combinations of two carbon (sucrose and molasses) and two nitrogen (urea and yeast extract) sources to optimize control of Botrytis cinerea on apple. All of the strains were grown in different liquid media (pH = 6.9) including: sucrose + yeast extract, molasses of sugar beet + yeast extract in 2:1 and 1:1 w/w ratios, molasses of sugar beet + urea, molasses, malt extract and nutrient broth. Apples (Golden Delicious) were inoculated by a 25-microl suspension of 10(6) spores of B. cinerea per ml, wounding each fruit (in two sites separately). Then a 25-microl suspension of each strain, containing 2 x 10(8) cfu ml(-1) grown in each of the above culture media, was applied to each wound. Results indicated that Molasses + Yeast extract (1:1 w/w) medium supported rapid growth in all of the strains. The final growth of B. subtilis B-16 in Molasses + Yeast extract (1:1 w/w) medium was 5 x 10(9) cfu ml(-1). After ten days, all of the strains significantly inhibited pathogenicity of B. cinerea on apples. The biocontrol efficacy of B. subtilis B-3 in Molasses + Yeast extract (1:1 w/w) medium reduced the severity of grey mould from 100% (inoculated control) to less than 26.9%. After 20 days, Strain B-3 showed a considerable biocontrol efficacy in Molasses medium and reduced the severity of grey mould from 100% (inoculated control) to less than 38.2%. The results obtained in this study could be used to provide a reliable basis for the increase of population of biocontrol agents in fermentation process.
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Claims 5
Claim · model-written summary
Molasses plus yeast extract medium supported rapid growth of all tested biocontrol strains.
- Taxon
- apple
Malus domesticat0 - Treatment
- Molasses + Yeast extract (1:1 w/w) mediume0
- Outcome
- rapid growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results indicated that Molasses + Yeast extract (1:1 w/w) medium supported rapid growth in all of the strains.”
t0“apple”
Claim · model-written summary
B. subtilis B-16 reached 5 x 10(9) cfu ml(-1) in molasses plus yeast extract medium.
- Taxon
- appleMalus domesticat0
- Treatment
- Molasses + Yeast extract (1:1 w/w) mediume0
- Interactor
- B. subtilis B-16
Bacillus subtilise0biocontrol_agent - Outcome
- final growth of B. subtilis B-16e0
- Value
- 5 x 10(9) cfu ml(-1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The final growth of B. subtilis B-16 in Molasses + Yeast extract (1:1 w/w) medium was 5 x 10(9) cfu ml(-1).”
t0“apple”
Claim · model-written summary
All tested strains significantly inhibited B. cinerea pathogenicity on apples after ten days.
- Taxon
- applesMalus domesticae0
- Treatment
- all of the strainse0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- pathogenicity of B. cinereae0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After ten days, all of the strains significantly inhibited pathogenicity of B. cinerea on apples.”
Claim · model-written summary
B. subtilis B-3 grown in molasses plus yeast extract reduced grey mould severity from 100% to less than 26.9%.
- Taxon
- appleMalus domesticat0
- Treatment
- B. subtilis B-3 in Molasses + Yeast extract (1:1 w/w) mediume0
- Interactor
- B. subtilis B-3Bacillus subtilise0biocontrol_agent
- Outcome
- severity of grey moulde0
- Direction
- reducede0
- Value
- less than 26.9%e0
- Qualifier
- 100%e0
- Comparator
- inoculated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The biocontrol efficacy of B. subtilis B-3 in Molasses + Yeast extract (1:1 w/w) medium reduced the severity of grey mould from 100% (inoculated control) to less than 26.9%.”
t0“apple”
Claim · model-written summary
After 20 days B-3 grown in molasses medium reduced grey mould severity from 100% to less than 38.2%.
- Taxon
- appleMalus domesticat0
- Treatment
- Strain B-3e0
- Interactor
- Strain B-3e0biocontrol_agent
- Outcome
- severity of grey moulde0
- Direction
- reducede0
- Value
- less than 38.2%e0
- Qualifier
- After 20 dayse0
- Comparator
- inoculated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 20 days, Strain B-3 showed a considerable biocontrol efficacy in Molasses medium and reduced the severity of grey mould from 100% (inoculated control) to less than 38.2%.”
t0“apple”
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.