Abstract
BACKGROUND: Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies. RESULTS: The present study reported a novel biotechnology allowing cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips. Following cryopreservation by droplet-vitrification or encapsulation-dehydration, about 62-67% of shoot regrowth and 100% of ASGV cryopreservation were obtained. Although shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration, continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus concentration to comparative levels as those produced by shoot tip culture (as a control to shoot tip cryopreservation). Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting and to a herbaceous indicator by mechanical inoculation. Gene sequencing in three fragments of ASGV genome including coat protein and movement protein showed that cryopreserved ASGV shared 99.87% nucleotide identities with shoot tip culture-preserved virus, indicating cryopreserved virus is genetically stable. CONCLUSIONS: The present study demonstrates ASGV, a representative virus that can infect meristematic cells of shoot tips, can be efficiently cryopreserved in shoot tips. To the best of our knowledge, this is the first report on plant virus cryopreservation in living tissues, and has great potential applications to long-term preservation of plant viruses.
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Claims 7
Claim · model-written summary
Shoot regrowth after cryopreservation by droplet-vitrification or encapsulation-dehydration was about 62-67%.
- Taxon
- Apple
Malus domesticae3 - Organ / stage
- shoot tipse3
- Treatment
- cryopreservation by droplet-vitrification or encapsulation-dehydratione1
- Outcome
- shoot regrowthe1
- Value
- 62-67%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Following cryopreservation by droplet-vitrification or encapsulation-dehydration, about 62-67% of shoot regrowth”
e3“cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips”
Claim · model-written summary
Cryopreservation of ASGV in shoot tips was obtained in 100% of cases.
- Taxon
- AppleMalus domesticae3
- Interactor
- ASGVCapillovirus malie2pathogen
- Outcome
- ASGV cryopreservatione2
- Value
- 100%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“about 62-67% of shoot regrowth and 100% of ASGV cryopreservation were obtained”
e3“cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips”
Claim · model-written summary
Shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration.
- Taxon
- AppleMalus domesticae3
- Outcome
- shoot proliferation and virus concentratione4
- Direction
- reducede4
- Qualifier
- after 8 weeks of shoot regeneratione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Although shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration”
e3“cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips”
Claim · model-written summary
Continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus concentration to levels comparable with shoot tip culture.
- Taxon
- AppleMalus domesticae3
- Treatment
- continuous subculture for 4 times (16 weeks)e5
- Outcome
- shoot proliferation and virus concentratione5
- Direction
- increasede5
- Qualifier
- to comparative levelse5
- Comparator
- shoot tip culturee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus concentration to comparative levels as those produced by shoot tip culture”
e3“cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips”
Claim · model-written summary
Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting.
Verbatim
e6“Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting and to a herbaceous indicator by mechanical inoculation”
Claim · model-written summary
Cryopreserved ASGV was efficiently transmitted to a herbaceous indicator by mechanical inoculation.
Verbatim
e6“Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting and to a herbaceous indicator by mechanical inoculation”
Claim · model-written summary
Cryopreserved ASGV shared 99.87% nucleotide identity with shoot tip culture-preserved virus in three genome fragments.
- Taxon
- AppleMalus domesticae3
- Interactor
- ASGVCapillovirus malie7pathogen
- Gene
- coat protein and movement proteine7
- Outcome
- nucleotide identitiese7
- Value
- 99.87%e7
- Comparator
- shoot tip culture-preserved viruse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Gene sequencing in three fragments of ASGV genome including coat protein and movement protein showed that cryopreserved ASGV shared 99.87% nucleotide identities with shoot tip culture-preserved virus”
e3“cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips”
The same questions in other papers
in vitro · disease · Malus domestica (apple)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.