Abstract
) is highly nutritious, rich in vitamin C, glucosinolates, and minerals. However, its high postharvest respiratory rate leads to rapid quality deterioration, particularly chlorophyll degradation and yellowing under ambient conditions. In China, the lack of timely pre-cooling facilities exacerbates postharvest losses. Therefore, developing safe, effective and low-cost preservation methods for broccoli during transportation is of great practical importance. In this study, RNA sequencing was employed to analyze the effects of preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments on gene expression in broccoli. Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention. Furthermore, chlorophyllide a oxygenase (CAO) was upregulated, while chlorophyll b reductase (CBR) was downregulated, suggesting modulation of the chlorophyll cycle. These findings elucidate the molecular mechanisms by which 1-MCP and pre-cooling combined with cold regulate chlorophyll metabolism, providing new insights into the gene regulatory network underlying the postharvest quality maintenance in broccoli.
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Claims 10
Claim · model-written summary
preharvest 1-methylcyclopropene (1-MCP) upregulated or maintained key genes involved in chlorophyll biosynthesis.
- Taxon
- Broccoli
Brassica oleracea var. italicat0 - Treatment
- preharvest 1-methylcyclopropene (1-MCP)e1
- Gene
- Glutamyl-tRNA reductase (GluTR)e0
- Outcome
- key genes involved in chlorophyll biosynthesise0
- Direction
- upregulated or maintainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
preharvest 1-methylcyclopropene (1-MCP) downregulated chlorophyll degradation-related genes.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- preharvest 1-methylcyclopropene (1-MCP)e1
- Gene
- Chlorophyllase (CLH)e0
- Outcome
- chlorophyll degradation-related genese0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
preharvest 1-methylcyclopropene (1-MCP) upregulated chlorophyllide a oxygenase (CAO).
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- preharvest 1-methylcyclopropene (1-MCP)e1
- Gene
- chlorophyllide a oxygenase (CAO)e0
- Outcome
- chlorophyllide a oxygenase (CAO)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, chlorophyllide a oxygenase (CAO) was upregulated, while chlorophyll b reductase (CBR) was downregulated, suggesting modulation of the chlorophyll cycle.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
preharvest 1-methylcyclopropene (1-MCP) downregulated chlorophyll b reductase (CBR).
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- preharvest 1-methylcyclopropene (1-MCP)e1
- Gene
- chlorophyll b reductase (CBR)e0
- Outcome
- chlorophyll b reductase (CBR)e0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, chlorophyllide a oxygenase (CAO) was upregulated, while chlorophyll b reductase (CBR) was downregulated, suggesting modulation of the chlorophyll cycle.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
preharvest 1-methylcyclopropene (1-MCP) enhanced chlorophyll retention.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- preharvest 1-methylcyclopropene (1-MCP)e1
- Outcome
- chlorophyll retentione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
postharvest pre-cooling combined with cold treatments upregulated or maintained key genes involved in chlorophyll biosynthesis.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- postharvest pre-cooling combined with cold treatmentse1
- Gene
- Glutamyl-tRNA reductase (GluTR)e0
- Outcome
- key genes involved in chlorophyll biosynthesise0
- Direction
- upregulated or maintainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
postharvest pre-cooling combined with cold treatments downregulated chlorophyll degradation-related genes.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- postharvest pre-cooling combined with cold treatmentse1
- Gene
- Chlorophyllase (CLH)e0
- Outcome
- chlorophyll degradation-related genese0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
postharvest pre-cooling combined with cold treatments upregulated chlorophyllide a oxygenase (CAO).
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- postharvest pre-cooling combined with cold treatmentse1
- Gene
- chlorophyllide a oxygenase (CAO)e0
- Outcome
- chlorophyllide a oxygenase (CAO)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, chlorophyllide a oxygenase (CAO) was upregulated, while chlorophyll b reductase (CBR) was downregulated, suggesting modulation of the chlorophyll cycle.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
postharvest pre-cooling combined with cold treatments downregulated chlorophyll b reductase (CBR).
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- postharvest pre-cooling combined with cold treatmentse1
- Gene
- chlorophyll b reductase (CBR)e0
- Outcome
- chlorophyll b reductase (CBR)e0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, chlorophyllide a oxygenase (CAO) was upregulated, while chlorophyll b reductase (CBR) was downregulated, suggesting modulation of the chlorophyll cycle.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
Claim · model-written summary
postharvest pre-cooling combined with cold treatments enhanced chlorophyll retention.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- postharvest pre-cooling combined with cold treatmentse1
- Outcome
- chlorophyll retentione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that both treatments significantly upregulated or maintained key genes involved in chlorophyll biosynthesis (e.g., Glutamyl-tRNA reductase (GluTR), porphobilinogen deaminase (PBGD), magnesium chelatase (MgCh)) and downregulated chlorophyll degradation-related genes (e.g., Chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PaO)), resulting in enhanced chlorophyll retention.”
e1“preharvest 1-methylcyclopropene (1-MCP) and postharvest pre-cooling combined with cold treatments”
t0“Broccoli”
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