Abstract
Harvesting fresh produce induces countless changes in different metabolic processes, initiating senescence of the plant tissue. Understanding the changes in protein expression and activity during postharvest senescence can help identify the networks that contribute to quality loss and shelf-life reduction in produce. Identifying these proteins can lead to strategies for extending fruits and vegetables' freshness and shelf life. This study investigated the proteome-level changes in harvested broccoli florets stored at either room temperature (25 °C) or in refrigerated conditions (4 °C) using Tandem Mass Tag technology (TMT). A lower storage temperature reduced the levels of proteins involved in ubiquitin-dependent and ubiquitin-independent protein degradation pathways mediated by the 20S and 26S proteasomes and in the RNA-Induced Silencing Complex (RISC) associated with the small interfering RNA (siRNA) pathway. Moreover, we identified protein kinases, transcription factors, and dehydration-related proteins, as well as mapped chemical interaction networks and key pathways active during broccoli senescence. Pathways related to autophagy, ribosome biosynthesis, and carbon metabolism were significantly downregulated under cold temperatures. Proteins related to chlorophyll and reactive oxygen species (ROS) pathways were differentially expressed in harvested broccoli stored at room temperature. The current report facilitates a comprehensive understanding of the complex regulatory orchestration that builds a protein network that could be used for future endeavors in improving the postharvest shelf life of broccoli.
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Claims 4
Claim · model-written summary
Cold storage reduced levels of proteins in proteasomal degradation and RNA silencing pathways.
- Taxon
- broccoli
Brassica oleracea var. italicat0 - Treatment
- A lower storage temperaturee0
- Outcome
- levels of proteins involved in ubiquitin-dependent and ubiquitin-independent protein degradation pathwayse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A lower storage temperature reduced the levels of proteins involved in ubiquitin-dependent and ubiquitin-independent protein degradation pathways mediated by the 20S and 26S proteasomes and in the RNA-Induced Silencing Complex (RISC) associated with the small interfering RNA (siRNA) pathway”
t0“broccoli”
Claim · model-written summary
The study identified kinases, transcription factors and dehydration proteins during broccoli senescence.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Outcome
- protein kinases, transcription factors, and dehydration-related proteinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified protein kinases, transcription factors, and dehydration-related proteins”
t0“broccoli”
Claim · model-written summary
Autophagy, ribosome biosynthesis and carbon metabolism pathways were significantly downregulated under cold storage.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- cold temperaturese0
- Outcome
- Pathways related to autophagy, ribosome biosynthesis, and carbon metabolisme0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pathways related to autophagy, ribosome biosynthesis, and carbon metabolism were significantly downregulated under cold temperatures”
t0“broccoli”
Claim · model-written summary
Chlorophyll and reactive oxygen species pathway proteins were differentially expressed at room temperature.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Outcome
- Proteins related to chlorophyll and reactive oxygen species (ROS) pathwayse0
- Setting
- room temperaturee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proteins related to chlorophyll and reactive oxygen species (ROS) pathways were differentially expressed in harvested broccoli stored at room temperature”
The same questions in other papers
cold · molecular: metabolites · Brassica oleracea (wild cabbage)Results differ
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
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See all 10 papers →storage · quality: protein · Brassica oleracea (wild cabbage)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.