Abstract
Glycine-rich RNA-binding proteins (GR-RBPs) play pivotal roles in regulating plant responses to abiotic and biotic stresses. However, the involvement of GR-RBPs in wheat immune responses to Puccinia striiformis f. sp. tritici ( Pst ) remains poorly understood. Here, we report the identification of 58 GR-RBP genes in wheat and provide a functional characterization of TaGR-RBP, which exhibits significant upregulation during wheat- Pst interactions. Subcellular localization analysis demonstrated that TaGR-RBP localizes in both the cytoplasm and the nucleus. Virus-induced gene silencing (VIGS) using Barley Stripe Mosaic Virus (BSMV) revealed that transient silencing of TaGR-RBP compromises wheat resistance to Pst . Furthermore, CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst, as evidenced by diminished hydrogen peroxide (H 2 O 2 ) accumulation and enhanced fungal proliferation, confirming its positive regulatory role in wheat immunity. Transient expression of TaGR-RBP in Nicotiana benthamiana induced Reactive Oxygen Species (ROS) accumulation. AlphaFold3 predictions and an RNA electrophoretic mobility shift assay (REMSA) validated the RNA-binding activity of TaGR-RBP. Overall, these findings imply the functional significance of GR-RBPs in wheat resistance against Pst . Deciphering the function of TaGR-RBP in plant immunity provides a theoretical basis for engineering disease-resistant wheat and guides the development of sustainable strategies for disease control.
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Claims 9
Claim · model-written summary
Researchers identified 58 wheat GR-RBP genes.
- Taxon
- wheat
Triticum aestivume0 - Gene
- GR-RBP genese0
- Outcome
- GR-RBP genese0
- Value
- 58e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of 58 GR-RBP genes in wheat”
Claim · model-written summary
TaGR-RBP was significantly upregulated during wheat–Pst interaction.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste0pathogen
- Gene
- TaGR-RBPe0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaGR-RBP, which exhibits significant upregulation during wheat- Pst interactions.”
Claim · model-written summary
TaGR-RBP localized in cytoplasm and nucleus.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaGR-RBPe0
- Outcome
- localizes in both the cytoplasm and the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaGR-RBP localizes in both the cytoplasm and the nucleus.”
t0“Wheat”
Claim · model-written summary
Transient TaGR-RBP silencing compromised wheat resistance to Pst.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transient silencing of TaGR-RBPe0
- Interactor
- Pste0pathogen
- Gene
- TaGR-RBPe0
- Outcome
- wheat resistance to Pste0
- Direction
- compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing of TaGR-RBP compromises wheat resistance to Pst”
t0“Wheat”
Claim · model-written summary
TaGR-RBP knockout reduced wheat resistance to Pst.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- CRISPR-Cas9-mediated knockout of TaGR-RBPe0
- Interactor
- Pste0pathogen
- Gene
- TaGR-RBPe0
- Outcome
- resistance to Pste0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst”
t0“Wheat”
Claim · model-written summary
TaGR-RBP knockout diminished hydrogen peroxide accumulation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockout of TaGR-RBPe0
- Interactor
- Pste0pathogen
- Outcome
- hydrogen peroxide (H 2 O 2 ) accumulatione0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst, as evidenced by diminished hydrogen peroxide (H 2 O 2 ) accumulation and enhanced fungal proliferation”
t0“Wheat”
e1“CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst, as evidenced by diminished hydrogen peroxide (H 2 O 2 ) accumulation and enhanced fungal proliferation”
Claim · model-written summary
TaGR-RBP knockout enhanced fungal proliferation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockout of TaGR-RBPe0
- Interactor
- Pste0pathogen
- Outcome
- fungal proliferatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst, as evidenced by diminished hydrogen peroxide (H 2 O 2 ) accumulation and enhanced fungal proliferation”
t0“Wheat”
e1“CRISPR-Cas9-mediated knockout of TaGR-RBP resulted in reduced resistance to Pst, as evidenced by diminished hydrogen peroxide (H 2 O 2 ) accumulation and enhanced fungal proliferation”
Claim · model-written summary
TaGR-RBP expression in Nicotiana benthamiana induced ROS accumulation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Transient expression of TaGR-RBPe0
- Gene
- TaGR-RBPe0
- Outcome
- Reactive Oxygen Species (ROS) accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of TaGR-RBP in Nicotiana benthamiana induced Reactive Oxygen Species (ROS) accumulation.”
Claim · model-written summary
TaGR-RBP RNA binding was validated.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaGR-RBPe0
- Outcome
- RNA-binding activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an RNA electrophoretic mobility shift assay (REMSA) validated the RNA-binding activity of TaGR-RBP.”
t0“Wheat”
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See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.