Abstract
C-type lectins (CTLs), a class of multifunctional proteins, are numerous in nematodes. One CTL gene, Mg01965, shown to be expressed in the subventral glands, especially in the second-stage juveniles of the root-knot nematode Meloidogyne graminicola, was further analysed in this study. In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots. Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast. Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22. The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages.
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Claims 4
Claim · model-written summary
RNA interference targeting Mg01965 in preparasitic juveniles of M. graminicola reduced their ability to infect host plant roots.
- Taxon
- host plante1
- Organ / stage
- preparasitic juvenilese1
- Treatment
- In vitro RNA interference targeting Mg01965e1
- Interactor
- M. graminicolaMeloidogyne graminicolae2pest
- Gene
- Mg01965e1
- Outcome
- ability to infect host plant rootse1
- Direction
- reducede1
Not reportedComparator
Verbatim
e1“In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots”
e2“Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast”
Claim · model-written summary
Mg01965 is secreted by M. graminicola into host roots during early parasitic stages and accumulates in the apoplast.
Verbatim
e1“In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots”
e2“Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast”
Claim · model-written summary
Transient expression of Mg01965 targeted to the apoplast suppressed the flg22-triggered burst of reactive oxygen species in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae3 - Treatment
- Transient expression of Mg01965e3
- Gene
- Mg01965e3
- Outcome
- the burst of reactive oxygen speciese3
- Direction
- suppressede3
- Qualifier
- triggered by flg22e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22”
Claim · model-written summary
Mg01965 suppresses plant innate immunity in the host apoplast and thereby promotes nematode parasitism in early infection.
Verbatim
e4“The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages”
The same questions in other papers
transgenic · physiology: oxidative · Nicotiana benthamianaPositive and negative results
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.