Abstract
Reactive oxygen species (ROS) are vital for plant immunity and regulation of their production is crucial for plant health. While the mechanisms that elicit ROS production have been relatively well studied, those that repress ROS generation are less well understood. Here, via screening Brachypodium distachyon RNA interference mutants, we identified BdWRKY19 as a negative regulator of ROS generation whose knockdown confers elevated resistance to the rust fungus Puccinia brachypodii. The three wheat paralogous genes TaWRKY19 are induced during infection by virulent P. striiformis f. sp. tritici (Pst) and have partially redundant roles in resistance. The stable overexpression of TaWRKY19 in wheat increased susceptibility to an avirulent Pst race, while mutations in all three TaWRKY19 copies conferred strong resistance to Pst by enhancing host plant ROS accumulation. We show that TaWRKY19 is a transcriptional repressor that binds to a W-box element in the promoter of TaNOX10, which encodes an NADPH oxidase and is required for ROS generation and host resistance to Pst. Collectively, our findings reveal that TaWRKY19 compromises wheat resistance to the fungal pathogen and suggest TaWRKY19 as a potential target to improve wheat resistance to the commercially important wheat stripe rust fungus.
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Claims 9
Claim · model-written summary
BdWRKY19 negatively regulated ROS generation in Brachypodium.
Not reportedTaxon
Verbatim
e0“we identified BdWRKY19 as a negative regulator of ROS generation whose knockdown confers elevated resistance to the rust fungus Puccinia brachypodii.”
Claim · model-written summary
BdWRKY19 knockdown elevated resistance to Puccinia brachypodii.
- Treatment
- knockdowne0
- Interactor
- Puccinia brachypodii
.jpg?width=60)
Puccinia brachypodiie0pathogen - Gene
- BdWRKY19e0
- Outcome
- resistancee0
- Direction
- elevatede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“BdWRKY19 as a negative regulator of ROS generation whose knockdown confers elevated resistance to the rust fungus Puccinia brachypodii.”
Claim · model-written summary
The three wheat TaWRKY19 paralogs were induced during virulent Pst infection.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- P. striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- TaWRKY19e0
- Outcome
- inducede0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The three wheat paralogous genes TaWRKY19 are induced during infection by virulent P. striiformis f. sp. tritici (Pst) and have partially redundant roles in resistance.”
Claim · model-written summary
Wheat TaWRKY19 paralogs had partially redundant roles in resistance.
- Taxon
- wheatTriticum aestivume0
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie0pathogen
- Gene
- TaWRKY19e0
- Outcome
- partially redundant roles in resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The three wheat paralogous genes TaWRKY19 are induced during infection by virulent P. striiformis f. sp. tritici (Pst) and have partially redundant roles in resistance.”
Claim · model-written summary
TaWRKY19 overexpression increased wheat susceptibility to an avirulent Pst race.
- Taxon
- wheatTriticum aestivume0
- Treatment
- stable overexpression of TaWRKY19e0
- Interactor
- Pste0pathogen
- Gene
- TaWRKY19e0
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stable overexpression of TaWRKY19 in wheat increased susceptibility to an avirulent Pst race”
Claim · model-written summary
Mutating all three TaWRKY19 copies conferred strong wheat resistance to Pst.
- Treatment
- mutations in all three TaWRKY19 copiese0
- Interactor
- Pste0pathogen
- Gene
- TaWRKY19e0
- Outcome
- resistancee0
- Direction
- stronge0
Not reportedTaxonComparator
Verbatim
e0“mutations in all three TaWRKY19 copies conferred strong resistance to Pst by enhancing host plant ROS accumulation.”
Claim · model-written summary
Mutating all three TaWRKY19 copies enhanced host plant ROS accumulation.
- Treatment
- mutations in all three TaWRKY19 copiese0
- Interactor
- Pste0pathogen
- Gene
- TaWRKY19e0
- Outcome
- host plant ROS accumulatione0
- Direction
- enhancinge0
Not reportedTaxonComparator
Verbatim
e0“mutations in all three TaWRKY19 copies conferred strong resistance to Pst by enhancing host plant ROS accumulation.”
Claim · model-written summary
TaWRKY19 represses transcription by binding a W-box element in the TaNOX10 promoter.
- Gene
- TaWRKY19e0
- Outcome
- binds to a W-box element in the promoter of TaNOX10e0
- Qualifier
- transcriptional repressore0
Not reportedTaxon
Verbatim
e0“TaWRKY19 is a transcriptional repressor that binds to a W-box element in the promoter of TaNOX10”
Claim · model-written summary
TaNOX10 is required for ROS generation and host resistance to Pst.
Not reportedTaxon
Verbatim
e0“TaNOX10, which encodes an NADPH oxidase and is required for ROS generation and host resistance to Pst.”
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