Abstract
Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection.
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Claims 10
Claim · model-written summary
Pst_12806 from the wheat stripe rust fungus is translocated into chloroplasts.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- Pst_12806e0
- Outcome
- translocated into chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts”
Claim · model-written summary
Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants.
- Taxon
- tobacco plants
Nicotiana tabacume1 - Treatment
- Transient expression of Pst_12806e1
- Gene
- Pst_12806e1
- Outcome
- BAX-induced cell deathe1
- Direction
- inhibitse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat”
Claim · model-written summary
Transient expression of Pst_12806 reduces Pseudomonas-induced hypersensitive response in wheat.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transient expression of Pst_12806e1
- Interactor
- Pseudomonas
Pseudomonase1pathogen - Outcome
- Pseudomonas-induced hypersensitive responsee1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat”
Claim · model-written summary
Pst_12806 suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Pst_12806e1
- Gene
- Pst_12806e1
- Outcome
- callose deposition and the expression of defense-related genese2
- Direction
- reducinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes”
e1“Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat”
Claim · model-written summary
Pst_12806 is upregulated during infection.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Pst_12806e3
- Outcome
- upregulated during infectione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation”
t0“An effector protein of the wheat stripe rust fungus”
Claim · model-written summary
Knockdown of Pst_12806 by host-induced gene silencing reduces Pst growth and development.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockdown (by host-induced gene silencing)e3
- Interactor
- Pste3pathogen
- Gene
- Pst_12806e3
- Outcome
- Pst growth and developmente3
- Direction
- reducese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation”
t0“An effector protein of the wheat stripe rust fungus”
Claim · model-written summary
The reduced Pst growth is likely due to increased ROS accumulation.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Pst_12806e3
- Compound
- ROSe3
- Outcome
- Pst growth and developmente3
- Qualifier
- likely due to increased ROS accumulatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation”
t0“An effector protein of the wheat stripe rust fungus”
Claim · model-written summary
Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein.
- Taxon
- wheatTriticum aestivume4
- Gene
- TaISPe4
- Outcome
- interacts with the C-terminal Rieske domaine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex)”
Claim · model-written summary
Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Expression of Pst_12806e5
- Gene
- Pst_12806e5
- Outcome
- electron transport rate, photosynthesis, and production of chloroplast-derived ROSe5
- Direction
- reducese5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS”
t0“An effector protein of the wheat stripe rust fungus”
Claim · model-written summary
Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development.
- Taxon
- wheatTriticum aestivume6
- Material
- susceptible wheat cultivare6
- Treatment
- Silencing TaISP by virus-induced gene silencinge6
- Interactor
- Pste6pathogen
- Gene
- TaISPe6
- Outcome
- fungal growth and uredinium developmente6
- Direction
- reducese6
- Qualifier
- suggesting an increase in resistance against Pst infectione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.