Paper · 2025 · Horticulturae

Exogenous ACC, ABA, and/or Ethylene Enhance Berry Color Without Reducing Postharvest Performance in ‘Benitaka’ and ‘Rubi’ Table Grapes

Aline Cristina de Aguiar; Bianca Liriel Martins Barbosa; Danielle Mieko Sakai; et al.

8
claims
15
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by

Abstract

The objective of this work was to assess the association of ACC (1-aminocyclopropane-1-carboxylic acid), S-ABA (abscisic acid), and ethephon on color development and anthocyanin accumulation in berries, as well as on other quality attributes of ‘Benitaka’ and ‘Rubi’ table grapes grown in a subtropical region, in addition to postharvest conservation of clusters and vine regrowth. As a statistical model, a randomized block design consisting of nine treatments and four replications was used. The treatments included different associations of ACC, S-ABA, and ethephon, by using the commercial formulations Accede®, ProTone®, and Ethrel® containing 400 g kg−1 of ACC, 100 g L−1 of S-ABA, and 720 g L−1 of ethephon, respectively. The total anthocyanins, berry color index (CIRG), physicochemical characteristics, and cluster color coverage were assessed weekly, while berry firmness was assessed at harvest. After being harvested, the clusters were placed under cold storage at 1.0 ± 1.0 °C, and after 45 days, their postharvest attributes were assessed, as well as the vine regrowth in the following season. The exogenous and combined application of compounds at véraison was demonstrated to be a strategy to trigger the development of color in ‘Benitaka’ and ‘Rubi’ table grapes. For the ‘Benitaka’ table grape, the clusters treated with the different combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alone resulted in the highest concentration of total anthocyanins and the highest CIRG means (4.90; 4.86; 4.82; 4.81, 4.73, and 4.70 mg g−1 for anthocyanins, and 6.12, 6.08, 5.97, 5.92, 5.85, and 5.74 for CIRG, respectively). For the ‘Rubi’ table grape, the combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 days, resulted in higher means of anthocyanins and CIRG (3.86, 3.51, and 3.40 mg g−1 for anthocyanins and 5.05, 4.68, 4.82, and 4.79 for CIRG, respectively). Furthermore, the firmness of the berries of both cultivars remained unchanged, and after 45 days of cold storage, no reduction in the quality of the evaluated postharvest attributes was found. It was concluded that a single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAV was sufficient to promote the accumulation of anthocyanins and resulted in an intense and uniform color in the berries for both varieties assessed, with no adverse impacts on the postharvest conservation of the clusters or on the regrowth of the vines. The significance of this research was to demonstrate that table grapes with insufficient skin color can be improved through a combination of S-ABA and ACC at lower concentrations of active ingredients.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In ‘Benitaka’ table grape, clusters treated with combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alone had the highest total anthocyanin concentrations (4.90; 4.86; 4.82; 4.81, 4.73 and 4.70 mg g−1).

Taxon
table grapee0
Material
Benitakae0
Treatment
combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alonee0
Outcome
total anthocyaninse0
Direction
higheste0
Value
4.90; 4.86; 4.82; 4.81, 4.73, and 4.70 mg g−1e0

Not reportedComparator

Verbatim

e0

“For the ‘Benitaka’ table grape, the clusters treated with the different combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alone resulted in the highest concentration of total anthocyanins and the highest CIRG means (4.90; 4.86; 4.82; 4.81, 4.73, and 4.70 mg g−1 for anthocyanins, and 6.12, 6.08, 5.97, 5.92, 5.85, and 5.74 for CIRG, respectively)”

✓ located verbatim in the source · abstract, characters 1281–1640 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In ‘Benitaka’ table grape, the same treatments gave the highest berry color index (CIRG) means (6.12, 6.08, 5.97, 5.92, 5.85 and 5.74).

Taxon
table grapee0
Material
Benitakae0
Treatment
combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alonee0
Outcome
CIRG meanse0
Direction
higheste0
Value
6.12, 6.08, 5.97, 5.92, 5.85, and 5.74e0

Not reportedComparator

Verbatim

e0

“For the ‘Benitaka’ table grape, the clusters treated with the different combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alone resulted in the highest concentration of total anthocyanins and the highest CIRG means (4.90; 4.86; 4.82; 4.81, 4.73, and 4.70 mg g−1 for anthocyanins, and 6.12, 6.08, 5.97, 5.92, 5.85, and 5.74 for CIRG, respectively)”

✓ located verbatim in the source · abstract, characters 1281–1640 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In ‘Rubi’ table grape, ACC with S-ABA at 7 DAV or ethephon with S-ABA at 7 and 14 days gave higher anthocyanin means (3.86, 3.51 and 3.40 mg g−1).

Taxon
table grapee0
Material
Rubie0
Treatment
combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 dayse0
Outcome
anthocyaninse0
Direction
highere0
Value
3.86, 3.51, and 3.40 mg g−1e0

Not reportedComparator

Verbatim

e0

“For the ‘Rubi’ table grape, the combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 days, resulted in higher means of anthocyanins and CIRG (3.86, 3.51, and 3.40 mg g−1 for anthocyanins and 5.05, 4.68, 4.82, and 4.79 for CIRG, respectively)”

✓ located verbatim in the source · abstract, characters 1642–1926 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In ‘Rubi’ table grape, the same treatments gave higher CIRG means (5.05, 4.68, 4.82 and 4.79).

Taxon
table grapee0
Material
Rubie0
Treatment
combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 dayse0
Outcome
CIRGe0
Direction
highere0
Value
5.05, 4.68, 4.82, and 4.79e0

Not reportedComparator

Verbatim

e0

“For the ‘Rubi’ table grape, the combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 days, resulted in higher means of anthocyanins and CIRG (3.86, 3.51, and 3.40 mg g−1 for anthocyanins and 5.05, 4.68, 4.82, and 4.79 for CIRG, respectively)”

✓ located verbatim in the source · abstract, characters 1642–1926 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Berry firmness of both cultivars remained unchanged after the exogenous application of the compounds.

Taxon
Table Grapest0
Treatment
exogenous and combined application of compounds at véraisone1
Outcome
firmness of the berriese0
Direction
remained unchangede0

Not reportedComparator

Verbatim

e0

“the firmness of the berries of both cultivars remained unchanged”

✓ located verbatim in the source · abstract, characters 1941–2005 · show in text ↑
e1

“The exogenous and combined application of compounds at véraison”

✓ located verbatim in the source · abstract, characters 1108–1171 · show in text ↑
t0

“‘Benitaka’ and ‘Rubi’ Table Grapes”

✓ located verbatim in the source · title, characters 100–134 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

After 45 days of cold storage, no reduction in the quality of the evaluated postharvest attributes was found in treated grape clusters.

Taxon
Table Grapest0
Treatment
exogenous and combined application of compounds at véraisone1
Outcome
quality of the evaluated postharvest attributese0
Direction
no reductione0
Qualifier
after 45 days of cold storagee0

Not reportedComparator

Verbatim

e0

“after 45 days of cold storage, no reduction in the quality of the evaluated postharvest attributes was found”

✓ located verbatim in the source · abstract, characters 2011–2119 · show in text ↑
e1

“The exogenous and combined application of compounds at véraison”

✓ located verbatim in the source · abstract, characters 1108–1171 · show in text ↑
t0

“‘Benitaka’ and ‘Rubi’ Table Grapes”

✓ located verbatim in the source · title, characters 100–134 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude that a single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAV sufficed to promote anthocyanin accumulation and give intense, uniform berry color in both varieties.

Taxon
Table Grapest0
Treatment
single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAVe0
Outcome
accumulation of anthocyaninse0

Verbatim

e0

“a single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAV was sufficient to promote the accumulation of anthocyanins and resulted in an intense and uniform color in the berries for both varieties assessed”

✓ located verbatim in the source · abstract, characters 2143–2357 · show in text ↑
t0

“‘Benitaka’ and ‘Rubi’ Table Grapes”

✓ located verbatim in the source · title, characters 100–134 · show in text ↑
Intervention effectauthor interpretationoriginal resultno effectmeasured

Claim · model-written summary

The ACC plus S-ABA application had no adverse impacts on postharvest conservation of clusters or on vine regrowth.

Taxon
Table Grapest0
Treatment
single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAVe1
Outcome
regrowth of the vinese0
Direction
no adverse impactse0

Not reportedComparator

Verbatim

e0

“with no adverse impacts on the postharvest conservation of the clusters or on the regrowth of the vines”

✓ located verbatim in the source · abstract, characters 2359–2462 · show in text ↑
e1

“a single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAV”

✓ located verbatim in the source · abstract, characters 2143–2210 · show in text ↑
t0

“‘Benitaka’ and ‘Rubi’ Table Grapes”

✓ located verbatim in the source · title, characters 100–134 · show in text ↑

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