Paper · 2025 · Global Agronomy Research Journal

Effect of Humic Acid on the Seed Germination, Plantlet and Seedling Growth of Summer Vegetables

Rida Nisar; Kashif Waseem; Muhammad Zubair; et al.

14
claims
17
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

The application of humic acid (HA) as a biostimulant in vegetable production has gained attention for its potential to enhance plant growth, soil health, and nutrient availability. This study investigated the effects of different HA concentrations (0, 4, 8, and 12 g/kg) on seed germination, seedling growth, and physiological parameters of cucumber, pumpkin, okra, brinjal (eggplant), and tomato under controlled conditions. Results indicated that HA application significantly influenced germination rates, plant height, leaf number, root development, and flowering patterns in a dose- and species-dependent manner. Lower HA concentrations (4 g/kg) improved germination speed in cucumber, pumpkin, and okra, whereas higher doses (12 g/kg) delayed germination or caused seedling mortality in okra and tomato. Root proliferation was enhanced across species, with the highest root numbers observed at 12 g/kg in cucumber and pumpkin. However, excessive HA (12 g/kg) adversely affected okra and tomato, leading to stunted growth or plant death. Brinjal seedlings showed moderate growth improvements with HA, while tomato seedlings exhibited delayed flowering under HA treatment. The findings suggest that optimal HA doses vary by crop species, with moderate concentrations (4–8 g/kg) generally promoting growth, while higher doses may induce stress. This study highlights the importance of tailored HA application strategies to maximize benefits in sustainable vegetable production.

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Claims 14

Intervention effectobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

Humic acid significantly affected germination, growth and flowering in a dose and species dependent manner.

Taxon
cucumber, pumpkin, okra, brinjal (eggplant), and tomatoe1
Treatment
HA applicatione0
Outcome
germination rates, plant height, leaf numbere0
Qualifier
dose- and species-dependent mannere0

Not reportedComparatorDirection

Verbatim

e0

“HA application significantly influenced germination rates, plant height, leaf number, root development, and flowering patterns in a dose- and species-dependent manner”

✓ located verbatim in the source · abstract, characters 449–615 · show in text ↑
e1

“cucumber, pumpkin, okra, brinjal (eggplant), and tomato”

✓ located verbatim in the source · abstract, characters 341–396 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 4 g/kg humic acid dose improved cucumber germination speed.

Taxon
cucumberCucumis sativuse0
Treatment
HA concentrations (4 g/kg)e0
Outcome
germination speede0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Lower HA concentrations (4 g/kg) improved germination speed in cucumber, pumpkin, and okra”

✓ located verbatim in the source · abstract, characters 617–707 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 4 g/kg humic acid dose improved pumpkin germination speed.

Taxon
pumpkine0
Treatment
HA concentrations (4 g/kg)e0
Outcome
germination speede0
Direction
improvede0

Not reportedComparator

Verbatim

e0

“Lower HA concentrations (4 g/kg) improved germination speed in cucumber, pumpkin, and okra”

✓ located verbatim in the source · abstract, characters 617–707 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 4 g/kg humic acid dose improved okra germination speed.

Treatment
HA concentrations (4 g/kg)e0
Outcome
germination speede0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Lower HA concentrations (4 g/kg) improved germination speed in cucumber, pumpkin, and okra”

✓ located verbatim in the source · abstract, characters 617–707 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A 12 g/kg dose delayed germination or killed okra seedlings.

Treatment
higher doses (12 g/kg)e0
Outcome
germination or caused seedling mortalitye0
Direction
delayede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“higher doses (12 g/kg) delayed germination or caused seedling mortality in okra and tomato”

✓ located verbatim in the source · abstract, characters 717–807 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A 12 g/kg dose delayed germination or killed tomato seedlings.

Treatment
higher doses (12 g/kg)e0
Outcome
germination or caused seedling mortalitye0
Direction
delayede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“higher doses (12 g/kg) delayed germination or caused seedling mortality in okra and tomato”

✓ located verbatim in the source · abstract, characters 717–807 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Root proliferation increased across the tested species under humic acid.

Taxon
cucumber, pumpkin, okra, brinjal (eggplant), and tomatoe1
Treatment
HAe1
Outcome
Root proliferatione0
Direction
enhancede0

Not reportedComparator

Verbatim

e0

“Root proliferation was enhanced across species”

✓ located verbatim in the source · abstract, characters 809–855 · show in text ↑
e1

“HA concentrations (0, 4, 8, and 12 g/kg) on seed germination, seedling growth, and physiological parameters of cucumber, pumpkin, okra, brinjal (eggplant), and tomato”

✓ located verbatim in the source · abstract, characters 230–396 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cucumber root number was highest at 12 g/kg.

Taxon
cucumberCucumis sativuse0
Treatment
12 g/kge0
Outcome
root numberse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest root numbers observed at 12 g/kg in cucumber and pumpkin”

✓ located verbatim in the source · abstract, characters 862–930 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pumpkin root number was highest at 12 g/kg.

Taxon
pumpkine0
Treatment
12 g/kge0
Outcome
root numberse0
Direction
higheste0

Not reportedComparator

Verbatim

e0

“the highest root numbers observed at 12 g/kg in cucumber and pumpkin”

✓ located verbatim in the source · abstract, characters 862–930 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Excessive humic acid stunted or killed okra.

Treatment
HA (12 g/kg)e0
Outcome
stunted growth or plant deathe0
Direction
adversely affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“excessive HA (12 g/kg) adversely affected okra and tomato, leading to stunted growth or plant death”

✓ located verbatim in the source · abstract, characters 941–1040 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Excessive humic acid stunted or killed tomato.

Treatment
HA (12 g/kg)e0
Outcome
stunted growth or plant deathe0
Direction
adversely affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“excessive HA (12 g/kg) adversely affected okra and tomato, leading to stunted growth or plant death”

✓ located verbatim in the source · abstract, characters 941–1040 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Brinjal seedlings showed moderate growth improvement with humic acid.

Treatment
HAe0
Outcome
growth improvementse0
Direction
improvementse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Brinjal seedlings showed moderate growth improvements with HA”

✓ located verbatim in the source · abstract, characters 1042–1103 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Humic acid delayed flowering in tomato seedlings.

Treatment
HA treatmente0
Outcome
floweringe0
Direction
delayede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“tomato seedlings exhibited delayed flowering under HA treatment”

✓ located verbatim in the source · abstract, characters 1111–1174 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest moderate 4–8 g/kg doses generally promote growth.

Taxon
cucumber, pumpkin, okra, brinjal (eggplant), and tomatoe1
Outcome
moderate concentrations (4–8 g/kg) generally promoting growthe0

Verbatim

e0

“moderate concentrations (4–8 g/kg) generally promoting growth”

✓ located verbatim in the source · abstract, characters 1246–1307 · show in text ↑
e1

“cucumber, pumpkin, okra, brinjal (eggplant), and tomato”

✓ located verbatim in the source · abstract, characters 341–396 · show in text ↑

other · growth · Solanum melongena (eggplant)Results differ

23 papers: 20 positive, 3 negative or no effect

19 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. no effect

19 papers more in this group

See all 23 papers →

other · phenology · Solanum lycopersicum (tomato)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

other · growth · Abelmoschus esculentus (okra)Positive and negative results

32 papers: 30 positive, 2 negative or no effect

29 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

28 papers more in this group

See all 32 papers →

other · growth · Cucumis sativus (cucumber)Positive and negative results

36 papers: 32 positive, 4 negative or no effect

31 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. no effect
  3. no effect

32 papers more in this group

See all 36 papers →

other · growth · Solanum lycopersicum (tomato)Positive and negative results

175 papers: 157 positive, 18 negative or no effect

17 papers in the same direction as this paper · 157 in the opposite direction

  1. positive
  2. positive
  3. positive

171 papers more in this group

See all 175 papers →

other · growth · Cucurbita (pumpkins)Results agree

12 papers: 12 positive, 0 negative or no effect

11 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.