Abstract
Long non-coding RNAs (lncRNAs) are involved in the resistance of plants to infection by pathogens via interactions with microRNAs (miRNAs). Long non-coding RNAs are cleaved by miRNAs to produce phased small interfering RNAs (phasiRNAs), which, as competing endogenous RNAs (ceRNAs), function as decoys for mature miRNAs, thus inhibiting their expression, and contain pre-miRNA sequences to produce mature miRNAs. However, whether lncRNAs and miRNAs mediate other molecular mechanisms during plant resistance to pathogens is unknown. In this study, as a positive regulator, Sl-lncRNA15492 from tomato (Solanum lycopersicum Zaofen No. 2) plants affected tomato resistance to Phytophthora infestans. Gain- and loss-of-function experiments and RNA ligase-mediated 5'-amplification of cDNA ends (RLM-5' RACE) also revealed that Sl-miR482a was negatively involved in tomato resistance by targeting Sl-NBS-LRR genes and that silencing of Sl-NBS-LRR1 decreased tomato resistance. Sl-lncRNA15492 inhibited the expression of mature Sl-miR482a, whose precursor was located within the antisense sequence of Sl-lncRNA15492. Further degradome analysis and additional RLM-5' RACE experiments verified that mature Sl-miR482a could also cleave Sl-lncRNA15492. These results provide a mechanism by which lncRNAs might inhibit precursor miRNA expression through antisense strands of lncRNAs, and demonstrate that Sl-lncRNA15492 and Sl-miR482a mutually inhibit the maintenance of Sl-NBS-LRR1 homeostasis during tomato resistance to P. infestans.
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Claims 7
Claim · model-written summary
Sl-lncRNA15492 positively regulated tomato resistance to Phytophthora infestans.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- Solanum lycopersicum Zaofen No. 2e0
- Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Gene
- Sl-lncRNA15492e0
- Outcome
- tomato resistance to Phytophthora infestanse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as a positive regulator, Sl-lncRNA15492 from tomato (Solanum lycopersicum Zaofen No. 2) plants affected tomato resistance to Phytophthora infestans”
Claim · model-written summary
Sl-miR482a negatively regulated tomato resistance by targeting Sl-NBS-LRR genes.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Gene
- Sl-miR482ae0
- Outcome
- tomato resistancee0
- Qualifier
- targeting Sl-NBS-LRR genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sl-miR482a was negatively involved in tomato resistance by targeting Sl-NBS-LRR genes”
t0“Phytophthora infestans”
Claim · model-written summary
Silencing Sl-NBS-LRR1 decreased tomato resistance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of Sl-NBS-LRR1e0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Gene
- Sl-NBS-LRR1e0
- Outcome
- tomato resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of Sl-NBS-LRR1 decreased tomato resistance”
t0“Phytophthora infestans”
Claim · model-written summary
Sl-lncRNA15492 inhibited mature Sl-miR482a expression.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- Sl-lncRNA15492e0
- Outcome
- expression of mature Sl-miR482ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sl-lncRNA15492 inhibited the expression of mature Sl-miR482a”
t0“Solanum lycopersicum”
Claim · model-written summary
The Sl-miR482a precursor lies within the antisense sequence of Sl-lncRNA15492.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- Sl-miR482ae1
- Outcome
- precursor was located within the antisense sequence of Sl-lncRNA15492e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whose precursor was located within the antisense sequence of Sl-lncRNA15492”
e1“expression of mature Sl-miR482a”
t0“Solanum lycopersicum”
Claim · model-written summary
Mature Sl-miR482a could cleave Sl-lncRNA15492.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- Sl-miR482ae0
- Outcome
- cleave Sl-lncRNA15492e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature Sl-miR482a could also cleave Sl-lncRNA15492”
t0“Solanum lycopersicum”
Claim · model-written summary
Sl-lncRNA15492 and Sl-miR482a mutually inhibit maintenance of Sl-NBS-LRR1 homeostasis during tomato resistance.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- Sl-lncRNA15492e0
- Outcome
- maintenance of Sl-NBS-LRR1 homeostasise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sl-lncRNA15492 and Sl-miR482a mutually inhibit the maintenance of Sl-NBS-LRR1 homeostasis during tomato resistance to P. infestans”
The same questions in other papers
loss of function · Phytophthora infestans · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 10 in the opposite direction
- positive
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.