Abstract
Background: Functional alleles of host plant susceptibility genes (S genes) can exacerbate the severity of diseases by enhancing pathogen compatibility. Genetic editing of the targeted host S genes has demonstrated remarkable efficacy in conferring broad-spectrum resistance across multiple crop species. We have previously identified a Golgi-localized Nucleotide Sugar Transporter 1 homolog (SlGONST1.1) in the host plant Solanum lycopersicum as a susceptibility gene towards late blight caused by Phytophthora infestans. Methods: In this study, we performed a detailed characterization of tissue-specific and P. infestans-inducible expression patterns of this gene, and the subcellular localization of its encoded protein product. Results: Similar to phenotypes of two reported Slgonst1.1 edited lines, two newly generated genetically edited lines of SlGONST1.1 demonstrated enhanced resistance against P. infestans without obvious growth and developmental abnormality. Phytohormonal quantifications and reactive oxygen species measurements showed that an Slgonst1.1 line had lower constitutive abscisic acid contents and depleted reactive oxygen species burst induced by pathogen-associated molecular pattern. Further comparative transcriptomic analyses revealed that the expression of defense-related genes is disproportionally up-regulated in the Slgonst1.1 line. Conclusions: In summary, our findings confirmed SlGONST1.1 as a functional host susceptibility gene towards late blight and shed light on the potential molecular mechanism underlying its function.
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Claims 6
Claim · model-written summary
Two newly generated SlGONST1.1 edited tomato lines showed enhanced resistance against Phytophthora infestans.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- genetically edited lines of SlGONST1.1e0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- SlGONST1.1e0
- Outcome
- resistance against P. infestanse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two newly generated genetically edited lines of SlGONST1.1 demonstrated enhanced resistance against P. infestans”
t0“Tomato”
Claim · model-written summary
The edited SlGONST1.1 tomato lines showed no obvious growth and developmental abnormality.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- genetically edited lines of SlGONST1.1e0
- Gene
- SlGONST1.1e0
- Outcome
- growth and developmental abnormalitye0
- Direction
- without obviouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two newly generated genetically edited lines of SlGONST1.1 demonstrated enhanced resistance against P. infestans without obvious growth and developmental abnormality”
t0“Tomato”
Claim · model-written summary
An Slgonst1.1 tomato line had lower constitutive abscisic acid contents.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Slgonst1.1 linee0
- Gene
- Slgonst1.1e0
- Outcome
- constitutive abscisic acid contentse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Slgonst1.1 line had lower constitutive abscisic acid contents”
t0“Tomato”
Claim · model-written summary
An Slgonst1.1 tomato line had a depleted reactive oxygen species burst induced by pathogen-associated molecular pattern.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Slgonst1.1 linee0
- Gene
- Slgonst1.1e0
- Outcome
- reactive oxygen species burste0
- Direction
- depletede0
- Qualifier
- induced by pathogen-associated molecular patterne0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Slgonst1.1 line had lower constitutive abscisic acid contents and depleted reactive oxygen species burst induced by pathogen-associated molecular pattern”
t0“Tomato”
Claim · model-written summary
Defense-related genes were disproportionally up-regulated in the Slgonst1.1 tomato line.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Slgonst1.1 linee0
- Gene
- Slgonst1.1e0
- Outcome
- expression of defense-related genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of defense-related genes is disproportionally up-regulated in the Slgonst1.1 line”
t0“Tomato”
Claim · model-written summary
The authors conclude that SlGONST1.1 is a functional host susceptibility gene towards late blight.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- SlGONST1.1e0
- Outcome
- functional host susceptibility gene towards late blighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our findings confirmed SlGONST1.1 as a functional host susceptibility gene towards late blight”
t0“Tomato”
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