Abstract
In cuttings, detached leaves or stems are exposed to many stresses during the root regeneration process. Here, we show that the detached Arabidopsis thaliana leaf can tolerate mild osmotic stress and still regenerate roots. Under stress conditions, wounding and stress upregulate the jasmonate (JA) signaling pathway transcription factor gene MYC2 and the abscisic acid (ABA) signaling pathway transcription factor gene ABA INSENSITIVE5 (ABI5). The MYC2-ABI5 complex upregulates the expression of β-GLUCOSIDASE18 (BGLU18), which releases ABA from ABA glucose ester, resulting in ABA accumulation in the detached leaf. Mutations in MYC2, ABI5, and BGLU18 lead to the loss of stress tolerance and defects in root regeneration under osmotic stress. The successive application of JA and ABA can enhance the root regeneration ability in Arabidopsis and poplar cuttings. Overall, the JA-mediated wound signaling pathway and the ABA-mediated stress signaling pathway collaboratively amplify ABA signals to protect root regeneration under stress conditions.
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Claims 12
Claim · model-written summary
Detached Arabidopsis leaves tolerated mild osmotic stress and regenerated roots.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- detached Arabidopsis thaliana leafe0
- Outcome
- regenerate rootse0
- Qualifier
- mild osmotic stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detached Arabidopsis thaliana leaf can tolerate mild osmotic stress and still regenerate roots”
Claim · model-written summary
Wounding and stress upregulated MYC2.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- wounding and stresse0
- Gene
- MYC2e0
- Outcome
- transcription factor gene MYC2e0
- Direction
- upregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounding and stress upregulate the jasmonate (JA) signaling pathway transcription factor gene MYC2”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
Wounding and stress upregulated ABI5.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- wounding and stresse0
- Gene
- ABA INSENSITIVE5 (ABI5)e0
- Outcome
- ABA INSENSITIVE5 (ABI5)e0
- Direction
- upregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounding and stress upregulate the jasmonate (JA) signaling pathway transcription factor gene MYC2 and the abscisic acid (ABA) signaling pathway transcription factor gene ABA INSENSITIVE5 (ABI5)”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
The MYC2-ABI5 complex upregulated BGLU18 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- MYC2-ABI5e0
- Outcome
- expression of β-GLUCOSIDASE18 (BGLU18)e0
- Direction
- upregulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MYC2-ABI5 complex upregulates the expression of β-GLUCOSIDASE18 (BGLU18)”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
BGLU18 released ABA from its glucose ester.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- β-GLUCOSIDASE18 (BGLU18)e0
- Compound
- ABA glucose estere0
- Outcome
- releases ABA from ABA glucose estere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“β-GLUCOSIDASE18 (BGLU18), which releases ABA from ABA glucose ester”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
ABA accumulated in detached leaves after release from its glucose ester.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- detached leafe0
- Outcome
- ABA accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in ABA accumulation in the detached leaf”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
MYC2 mutation caused loss of stress tolerance and defective root regeneration under osmotic stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- Mutations in MYC2e0
- Gene
- MYC2e0
- Outcome
- loss of stress tolerance and defects in root regeneratione0
- Direction
- losse0
- Qualifier
- under osmotic stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in MYC2, ABI5, and BGLU18 lead to the loss of stress tolerance and defects in root regeneration under osmotic stress”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
ABI5 mutation caused loss of stress tolerance and defective root regeneration under osmotic stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ABI5e0
- Gene
- ABI5e0
- Outcome
- loss of stress tolerance and defects in root regeneratione0
- Direction
- losse0
- Qualifier
- under osmotic stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in MYC2, ABI5, and BGLU18 lead to the loss of stress tolerance and defects in root regeneration under osmotic stress”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
BGLU18 mutation caused loss of stress tolerance and defective root regeneration under osmotic stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- BGLU18e0
- Gene
- BGLU18e0
- Outcome
- loss of stress tolerance and defects in root regeneratione0
- Direction
- losse0
- Qualifier
- under osmotic stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in MYC2, ABI5, and BGLU18 lead to the loss of stress tolerance and defects in root regeneration under osmotic stress”
e1“detached Arabidopsis thaliana leaf”
Claim · model-written summary
Successive JA and ABA application enhanced root regeneration in Arabidopsis cuttings.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- cuttingse0
- Treatment
- successive application of JA and ABAe0
- Outcome
- root regeneration abilitye0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The successive application of JA and ABA can enhance the root regeneration ability in Arabidopsis and poplar cuttings”
Claim · model-written summary
Successive JA and ABA application enhanced root regeneration in poplar cuttings.
- Organ / stage
- cuttingse0
- Treatment
- successive application of JA and ABAe0
- Outcome
- root regeneration abilitye0
- Direction
- enhancee0
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The successive application of JA and ABA can enhance the root regeneration ability in Arabidopsis and poplar cuttings”
Claim · model-written summary
JA wound and ABA stress signaling together amplified ABA signals that protect root regeneration.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Compound
- ABAe0
- Outcome
- root regeneratione0
- Qualifier
- collaboratively amplify ABA signalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the JA-mediated wound signaling pathway and the ABA-mediated stress signaling pathway collaboratively amplify ABA signals to protect root regeneration under stress conditions”
e1“detached Arabidopsis thaliana leaf”
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