Abstract
Histone acetylation is an essential process in the epigenetic regulation of diverse biological processes, including environmental stress responses in plants. Previously, our research group identified a histone deacetylase (HDAC) inhibitor (HDI) that confers salt tolerance in Arabidopsis (Arabidopsis thaliana). In this study, we demonstrate that class I HDAC (HDA19) and class II HDACs (HDA5/14/15/18) control responses to salt stress through different pathways. The screening of 12 different selective HDIs indicated that seven newly reported HDIs enhance salt tolerance. Genetic analysis, based on a pharmacological study, identified which HDACs function in salinity stress tolerance. In the wild-type Columbia-0 background, hda19 plants exhibit tolerance to high-salinity stress, while hda5/14/15/18 plants exhibit hypersensitivity to salt stress. Transcriptome analysis revealed that the effect of HDA19 deficiency on the response to salinity stress is distinct from that of HDA5/14/15/18 deficiencies. In hda19 plants, the expression levels of stress tolerance-related genes, late embryogenesis abundant proteins that prevent protein aggregation and positive regulators such as ABI5 and NAC019 in abscisic acid signaling, were induced strongly relative to the wild type. Neither of these elements was up-regulated in the hda5/14/15/18 plants. The mutagenesis of HDA19 by genome editing in the hda5/14/15/18 plants enhanced salt tolerance, suggesting that suppression of HDA19 masks the phenotype caused by the suppression of class II HDACs in the salinity stress response. Collectively, our results demonstrate that HDIs that inhibit class I HDACs allow the rescue of plants from salinity stress regardless of their selectivity, and they provide insight into the hierarchal regulation of environmental stress responses through HDAC isoforms.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
hda19 plants exhibit tolerance to high-salinity stress relative to wild type.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Material
- hda19 plantse2
- Treatment
- high-salinity stresse2
- Outcome
- tolerancee2
- Direction
- exhibite2
- Comparator
- wild-type Columbia-0e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e2“In the wild-type Columbia-0 background, hda19 plants exhibit tolerance to high-salinity stress”
Claim · model-written summary
hda5/14/15/18 plants exhibit hypersensitivity to salt stress.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- hda5/14/15/18 plantse3
- Treatment
- salt stresse3
- Outcome
- hypersensitivity to salt stresse3
- Direction
- exhibite3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e3“while hda5/14/15/18 plants exhibit hypersensitivity to salt stress”
Claim · model-written summary
Seven newly reported HDIs enhance salt tolerance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Treatment
- seven newly reported HDIse1
- Outcome
- salt tolerancee1
- Direction
- enhancee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e1“The screening of 12 different selective HDIs indicated that seven newly reported HDIs enhance salt tolerance”
Claim · model-written summary
The effect of HDA19 deficiency on the response to salinity stress is distinct from that of HDA5/14/15/18 deficiencies.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Gene
- HDA19e4
- Outcome
- response to salinity stresse4
- Direction
- distincte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e4“Transcriptome analysis revealed that the effect of HDA19 deficiency on the response to salinity stress is distinct from that of HDA5/14/15/18 deficiencies”
Claim · model-written summary
Expression of stress tolerance-related genes ABI5 and NAC019 was induced strongly in hda19 plants relative to wild type.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- hda19 plantse5
- Gene
- ABI5 and NAC019e5
- Outcome
- expression levelse5
- Direction
- induced stronglye5
- Comparator
- the wild typee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e5“In hda19 plants, the expression levels of stress tolerance-related genes, late embryogenesis abundant proteins that prevent protein aggregation and positive regulators such as ABI5 and NAC019 in abscisic acid signaling, were induced strongly relative to the wild type”
Claim · model-written summary
Neither late embryogenesis abundant proteins nor ABI5 and NAC019 were up-regulated in hda5/14/15/18 plants.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- hda5/14/15/18 plantse6
- Outcome
- elementse6
- Direction
- was up-regulatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e6“Neither of these elements was up-regulated in the hda5/14/15/18 plants”
Claim · model-written summary
Mutagenesis of HDA19 by genome editing in hda5/14/15/18 plants enhanced salt tolerance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- hda5/14/15/18 plantse7
- Treatment
- mutagenesis of HDA19 by genome editinge7
- Outcome
- salt tolerancee7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e7“The mutagenesis of HDA19 by genome editing in the hda5/14/15/18 plants enhanced salt tolerance”
Claim · model-written summary
Suppression of HDA19 masks the phenotype caused by suppression of class II HDACs in the salinity stress response.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Gene
- HDA19e1
- Outcome
- phenotypee1
- Qualifier
- suppression of HDA19 masks the phenotype caused by the suppression of class II HDACse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e1“suppression of HDA19 masks the phenotype caused by the suppression of class II HDACs”
Claim · model-written summary
HDIs that inhibit class I HDACs allow rescue of plants from salinity stress regardless of their selectivity.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Treatment
- HDIs that inhibit class I HDACse1
- Outcome
- rescue of plants from salinity stresse1
- Direction
- allowe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt tolerance in Arabidopsis (Arabidopsis thaliana)”
e1“HDIs that inhibit class I HDACs allow the rescue of plants from salinity stress regardless of their selectivity”
The same questions in other papers
EC 3.5.1.98 (histone deacetylase) inhibitor · tolerance: abiotic · Arabidopsis thaliana (thale cress)Results agree
1 paper in the same direction as this paper
- positive
loss of function · tolerance: abiotic · Arabidopsis thaliana (thale cress)Positive and negative results
10 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.