Abstract
Summary Rice grains typically contain relatively high levels of toxic arsenic (As) but low levels of essential micronutrients. Biofortification of essential micronutrients while decreasing As accumulation in rice would benefit human nutrition and health. We generated transgenic rice expressing a gain‐of‐function mutant allele astol1 driven by the OsGPX1 promoter. astol1 encodes a plastid‐localized O ‐acetylserine (thiol) lyase (OAS‐TL) with Ser189Asn substitution (OsASTOL1 S189N ), which enhances cysteine biosynthesis by forming an indissociable cysteine synthase complex with its partner serine acetyltransferase (SAT). The effects on growth, As tolerance, and nutrient and As accumulation in rice grain were evaluated in hydroponic, pot and field experiments. The expression of OsASTOL1 S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As. The expression of OsASTOL1 S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield. Our study provides a new strategy to genetically engineer rice to biofortify multiple essential nutrients, reducing As accumulation in rice grain and enhancing As tolerance simultaneously.
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Claims 10
Claim · model-written summary
The mutant OAS-TL enhances cysteine biosynthesis through an indissociable complex with SAT.
- Taxon
- rice
Oryza sativat0 - Gene
- OsASTOL1 S189Ne0
- Outcome
- enhances cysteine biosynthesise0
- Qualifier
- forming an indissociable cysteine synthase complex with its partner serine acetyltransferase (SAT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“astol1 encodes a plastid‐localized O ‐acetylserine (thiol) lyase (OAS‐TL) with Ser189Asn substitution (OsASTOL1 S189N ), which enhances cysteine biosynthesis by forming an indissociable cysteine synthase complex with its partner serine acetyltransferase (SAT)”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression enhanced SAT activity.
- Taxon
- riceOryza sativat0
- Material
- pOsGPX1::astol1 transgenic linese0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- SAT activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression enhanced sulphate uptake.
- Taxon
- riceOryza sativat0
- Material
- pOsGPX1::astol1 transgenic linese0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- sulphate uptakee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression enhanced biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine.
- Taxon
- riceOryza sativat0
- Material
- pOsGPX1::astol1 transgenic linese0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- biosynthesis of cysteine, glutathione, phytochelatins and nicotianaminee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression enhanced tolerance to As.
- Taxon
- riceOryza sativat0
- Material
- pOsGPX1::astol1 transgenic linese0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- tolerance to Ase0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N in pOsGPX1::astol1 transgenic lines enhanced SAT activity, sulphate uptake, biosynthesis of cysteine, glutathione, phytochelatins and nicotianamine, and enhanced tolerance to As”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression decreased rice grain As accumulation.
- Taxon
- riceOryza sativat0
- Organ / stage
- rice graine0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- As accumulatione0
- Direction
- decreasede0
- Setting
- pot experiment and two field experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression increased rice grain macronutrients, especially sulphur, nitrogen and potassium.
- Taxon
- riceOryza sativat0
- Organ / stage
- rice graine0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- accumulation of multiple macronutrientse0
- Direction
- increasede0
- Qualifier
- especially sulphur, nitrogen and potassiume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression increased rice grain micronutrients, especially zinc and selenium.
- Taxon
- riceOryza sativat0
- Organ / stage
- rice graine0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- micronutrientse0
- Direction
- increasede0
- Qualifier
- especially zinc and seleniume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield”
t0“rice”
Claim · model-written summary
OsASTOL1 S189N expression had little effect on plant growth and grain yield.
- Taxon
- riceOryza sativat0
- Treatment
- expression of OsASTOL1 S189Ne0
- Gene
- OsASTOL1 S189Ne0
- Outcome
- plant growth and grain yielde0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsASTOL1 S189N decreased As accumulation while increased the accumulation of multiple macronutrients (especially sulphur, nitrogen and potassium) and micronutrients (especially zinc and selenium) in rice grain in a pot experiment and two field experiments, and had little effect on plant growth and grain yield”
t0“rice”
Claim · model-written summary
The authors propose mutant OAS-TL expression for simultaneous nutrient biofortification, lower grain As and increased As tolerance.
- Taxon
- riceOryza sativat0
- Outcome
- new strategy to genetically engineer rice to biofortify multiple essential nutrients, reducing As accumulation in rice grain and enhancing As tolerance simultaneouslye0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our study provides a new strategy to genetically engineer rice to biofortify multiple essential nutrients, reducing As accumulation in rice grain and enhancing As tolerance simultaneously”
t0“rice”
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