Abstract
We generated rice lines with increased content of nicotianamine (NA), a key ligand for metal transport and homeostasis. This was accomplished by activation tagging of rice nicotianamine synthase 2 (OsNAS2). Enhanced expression of the gene resulted in elevated NA levels, greater Zn accumulations and improved plant tolerance to a Zn deficiency. Expression of Zn-uptake genes and those for the biosynthesis of phytosiderophores (PS) were increased in transgenic plants. This suggests that the higher amount of NA led to greater exudation of PS from the roots, as well as stimulated Zn uptake, translocation and seed-loading. In the endosperm, the OsNAS2 activation-tagged line contained up to 20-fold more NA and 2.7-fold more zinc. Liquid chromatography combined with inductively coupled plasma mass spectrometry revealed that the total content of zinc complexed with NA and 2'-deoxymugineic acid was increased 16-fold. Mice fed with OsNAS2-D1 seeds recovered more rapidly from a zinc deficiency than did control mice receiving WT seeds. These results demonstrate that the level of bio-available zinc in rice grains can be enhanced significantly by activation tagging of OsNAS2.
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Claims 7
Claim · model-written summary
Enhanced OsNAS2 expression in rice resulted in elevated NA levels, greater Zn accumulation and improved tolerance to Zn deficiency.
- Taxon
- rice
Oryza sativae1 - Treatment
- Enhanced expression of the genee0
- Gene
- OsNAS2e1
- Outcome
- elevated NA levels, greater Zn accumulations and improved plant tolerance to a Zn deficiencye0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced expression of the gene resulted in elevated NA levels, greater Zn accumulations and improved plant tolerance to a Zn deficiency”
e1“activation tagging of rice nicotianamine synthase 2 (OsNAS2)”
Claim · model-written summary
Expression of Zn-uptake genes and phytosiderophore biosynthesis genes was increased in the transgenic rice plants.
- Taxon
- riceOryza sativat0
- Treatment
- transgenic plantse2
- Gene
- Zn-uptake genese2
- Outcome
- Expression of Zn-uptake genes and those for the biosynthesis of phytosiderophores (PS)e2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Expression of Zn-uptake genes and those for the biosynthesis of phytosiderophores (PS) were increased in transgenic plants”
t0“rice”
Claim · model-written summary
The authors suggest that the higher NA amount led to greater PS exudation from roots and stimulated Zn uptake, translocation and seed-loading in rice.
- Taxon
- riceOryza sativat0
- Compound
- NAe3
- Outcome
- greater exudation of PS from the rootse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“the higher amount of NA led to greater exudation of PS from the roots, as well as stimulated Zn uptake, translocation and seed-loading”
t0“rice”
Claim · model-written summary
In the endosperm, the OsNAS2 activation-tagged line contained up to 20-fold more NA.
- Taxon
- riceOryza sativat0
- Material
- OsNAS2 activation-tagged linee4
- Organ / stage
- endosperme4
- Outcome
- NAe4
- Direction
- moree4
- Value
- 20-folde4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In the endosperm, the OsNAS2 activation-tagged line contained up to 20-fold more NA and 2.7-fold more zinc”
t0“rice”
Claim · model-written summary
In the endosperm, the OsNAS2 activation-tagged line contained 2.7-fold more zinc.
- Taxon
- riceOryza sativat0
- Material
- OsNAS2 activation-tagged linee4
- Organ / stage
- endosperme4
- Outcome
- zince4
- Direction
- moree4
- Value
- 2.7-folde4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In the endosperm, the OsNAS2 activation-tagged line contained up to 20-fold more NA and 2.7-fold more zinc”
t0“rice”
Claim · model-written summary
The total content of zinc complexed with NA and 2'-deoxymugineic acid was increased 16-fold in the activation-tagged rice.
- Taxon
- riceOryza sativat0
- Outcome
- total content of zinc complexed with NA and 2'-deoxymugineic acide5
- Direction
- increasede5
- Value
- 16-folde5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the total content of zinc complexed with NA and 2'-deoxymugineic acid was increased 16-fold”
t0“rice”
Claim · model-written summary
Mice fed OsNAS2-D1 rice seeds recovered more rapidly from zinc deficiency than control mice fed wild-type seeds.
- Treatment
- fed with OsNAS2-D1 seedse6
- Outcome
- recovered more rapidly from a zinc deficiencye6
- Direction
- more rapidlye6
- Comparator
- control mice receiving WT seedse6
MusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Mice fed with OsNAS2-D1 seeds recovered more rapidly from a zinc deficiency than did control mice receiving WT seeds”
The same questions in other papers
transgenic · disease · Oryza sativa (rice)Positive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- positive
- no effect
- negative
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.