Abstract
Cuticular wax is a class of organic compounds that comprises the outermost layer of plant surfaces. Plant cuticular wax, the last barrier of self-defense, plays an important role in plant growth and development. The OsGL1-6 gene, a member of the fatty aldehyde decarbonylase gene family, is highly homologous to Arabidopsis CER1, which is involved in cuticular wax biosynthesis. However, whether OsGL1-6 participates in cuticular wax biosynthesis remains unknown. In this study, an OsGL1-6 antisense-RNA vector driven by its own promoter was constructed and introduced into the rice variety Zhonghua11 by Agrobacterium-mediated transformation to obtain several independent transgenic plants with decreased OsGL1-6 expression. These OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage, significantly decreased leaf cuticular wax deposition, thinner cuticle membrane, increased chlorophyll leaching and water loss rates, and enhanced drought sensitivity. The OsGL1-6 gene was constitutively expressed in all examined organs and was very highly expressed in leaf epidermal cells and vascular bundles. The transient expression of OsGL1-6-GFP fusion indicated that OsGL1-6 is localized in the endoplasmic reticulum. Qualitative and quantitative analysis of the wax composition using gas chromatography-mass spectrometry revealed a significantly reduced total cuticular wax load on the leaf blades of the OsGL1-6 antisense-RNA transgenic plants as well as markedly decreased alkane and aldehyde contents. Their primary alcohol contents increased significantly compared with those in the wild type plants, suggesting that OsGL1-6 is associated with the decarbonylation pathways in wax biosynthesis. We propose that OsGL1-6 is involved in the accumulation of leaf cuticular wax and directly impacts drought resistance in rice.
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Claims 12
Claim · model-written summary
OsGL1-6 antisense rice plants had droopy leaves at booting.
- Taxon
- Rice
Oryza sativat0 - Material
- OsGL1-6 antisense-RNA transgenic plantse0
- Organ / stage
- booting stagee0
- Outcome
- droopy leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage”
t0“Rice”
Claim · model-written summary
OsGL1-6 antisense rice plants had significantly decreased leaf cuticular wax deposition.
- Taxon
- RiceOryza sativat0
- Treatment
- OsGL1-6 antisense-RNAe0
- Gene
- OsGL1-6e0
- Outcome
- leaf cuticular wax depositione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage, significantly decreased leaf cuticular wax deposition”
t0“Rice”
Claim · model-written summary
OsGL1-6 antisense plants had a thinner cuticle membrane.
- Taxon
- RiceOryza sativat0
- Outcome
- cuticle membranee0
- Direction
- thinnere0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thinner cuticle membrane, increased chlorophyll leaching and water loss rates”
t0“Rice”
Claim · model-written summary
OsGL1-6 antisense plants showed increased chlorophyll leaching and water loss rates.
- Taxon
- RiceOryza sativat0
- Outcome
- chlorophyll leaching and water loss ratese0
- Direction
- increasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased chlorophyll leaching and water loss rates”
t0“Rice”
Claim · model-written summary
OsGL1-6 antisense plants had enhanced drought sensitivity.
- Taxon
- RiceOryza sativat0
- Outcome
- drought sensitivitye0
- Direction
- enhancede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced drought sensitivity”
t0“Rice”
Claim · model-written summary
OsGL1-6 was expressed in all examined organs, especially leaf epidermal cells and vascular bundles.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leaf epidermal cells and vascular bundlese0
- Gene
- OsGL1-6e0
- Outcome
- constitutively expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsGL1-6 gene was constitutively expressed in all examined organs and was very highly expressed in leaf epidermal cells and vascular bundles”
t0“Rice”
Claim · model-written summary
OsGL1-6 was localized in the endoplasmic reticulum.
- Taxon
- RiceOryza sativat0
- Gene
- OsGL1-6e0
- Outcome
- localized in the endoplasmic reticulume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGL1-6 is localized in the endoplasmic reticulum”
t0“Rice”
Claim · model-written summary
Total cuticular wax load was significantly reduced on antisense plant leaf blades.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leaf bladese0
- Treatment
- OsGL1-6 antisense-RNA transgenic plantse0
- Outcome
- total cuticular wax loade0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significantly reduced total cuticular wax load on the leaf blades of the OsGL1-6 antisense-RNA transgenic plants”
t0“Rice”
Claim · model-written summary
Alkane and aldehyde contents were markedly decreased in antisense plants.
- Taxon
- RiceOryza sativat0
- Outcome
- alkane and aldehyde contentse0
- Direction
- decreasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“markedly decreased alkane and aldehyde contents”
t0“Rice”
Claim · model-written summary
Primary alcohol contents increased significantly in antisense plants relative to wild type.
- Taxon
- RiceOryza sativat0
- Outcome
- primary alcohol contentse0
- Direction
- increasede0
- Comparator
- wild type plantse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their primary alcohol contents increased significantly compared with those in the wild type plants”
t0“Rice”
Claim · model-written summary
The authors infer OsGL1-6 participates in decarbonylation pathways in wax biosynthesis.
- Taxon
- RiceOryza sativat0
- Gene
- OsGL1-6e0
- Outcome
- decarbonylation pathways in wax biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that OsGL1-6 is associated with the decarbonylation pathways in wax biosynthesis”
t0“Rice”
Claim · model-written summary
The authors propose OsGL1-6 affects leaf cuticular wax accumulation and drought resistance in rice.
- Taxon
- RiceOryza sativat0
- Gene
- OsGL1-6e0
- Outcome
- accumulation of leaf cuticular wax and directly impacts drought resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that OsGL1-6 is involved in the accumulation of leaf cuticular wax and directly impacts drought resistance in rice”
t0“Rice”
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