Abstract
Rice tungro disease (RTD) is a significant yield constraint in rice-growing areas of South and Southeast Asia. Disease symptoms are caused largely by infection by the rice tungro bacilliform virus (RTBV). Two host transcription factors, RF2a and RF2b, regulate expression of the RTBV promoter and are important for plant development. Expression of a dominant negative mutant of these factors in transgenic rice resulted in phenotypes that mimic the symptoms of RTD, whereas overexpression of RF2a and RF2b had essentially no impact on plant development. Conversely, lines with elevated expression of RF2a or RF2b showed weak or no symptoms of infection after Agrobacterium inoculation of RTBV, whereas control plants showed severe stunting and leaf discoloration. Furthermore, transgenic plants exhibited reduced accumulation of RTBV RNA and viral DNA compared with nontransgenic plants. Similar results were obtained in studies after virus inoculation by green leafhoppers. Gaining disease resistance by elevating the expression of host regulators provides another strategy against RTD and may have implications for other pararetrovirus infections.
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Claims 5
Claim · model-written summary
Dominant negative RF2 factors produced disease-like phenotypes in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- Expression of a dominant negative mutante0
- Interactor
- RTDe0pathogen
- Outcome
- phenotypes that mimic the symptoms of RTDe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of a dominant negative mutant of these factors in transgenic rice resulted in phenotypes that mimic the symptoms of RTD”
Claim · model-written summary
RF2a and RF2b overexpression had essentially no developmental impact.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of RF2a and RF2be0
- Outcome
- plant developmente0
- Direction
- no impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of RF2a and RF2b had essentially no impact on plant development”
t0“rice plants”
Claim · model-written summary
Elevated RF2a or RF2b expression reduced symptoms after RTBV inoculation versus controls.
- Taxon
- riceOryza sativat0
- Treatment
- elevated expression of RF2a or RF2be0
- Interactor
- RTBVRice tungro bacilliform viruse0pathogen
- Outcome
- symptoms of infectione0
- Direction
- weak or noe0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lines with elevated expression of RF2a or RF2b showed weak or no symptoms of infection after Agrobacterium inoculation of RTBV, whereas control plants showed severe stunting and leaf discoloration”
t0“rice plants”
Claim · model-written summary
Transgenic plants accumulated less RTBV RNA and viral DNA than nontransgenic rice.
- Taxon
- riceOryza sativat0
- Treatment
- transgenic plantse0
- Interactor
- RTBVRice tungro bacilliform viruse0pathogen
- Outcome
- RTBV RNA and viral DNAe0
- Direction
- reducede0
- Comparator
- nontransgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants exhibited reduced accumulation of RTBV RNA and viral DNA compared with nontransgenic plants”
t0“rice plants”
Claim · model-written summary
Virus inoculation by green leafhoppers gave similar resistance results.
- Taxon
- riceOryza sativat0
- Interactor
- green leafhopperse0pest
- Outcome
- Similar resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar results were obtained in studies after virus inoculation by green leafhoppers.”
t0“rice plants”
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