Abstract
Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. Taking a lead from glyphosate-resistant weeds, two mutant variants of the rice EPSPS gene were developed by amino acid substitution (T173I + P177S; TIPS-OsEPSPS and G172A + T173I + P177S; GATIPS-OsEPSPS). These mutated EPSPS genes were overexpressed in rice under the control of either native EPSPS or constitutive promoters (maize ubiquitin [ZmUbi] promoter). The overexpression of TIPS-OsEPSPS under the control of the ZmUbi promoter resulted in higher tolerance to glyphosate (up to threefold of the recommended dose) without affecting the fitness and related agronomic traits of plants in both controlled and field conditions. Furthermore, such rice lines produced 17%-19% more grains compared to the wild type (WT) in the absence of glyphosate application and the phenylalanine and tryptophan contents in the transgenic seeds were found to be significantly higher in comparison with WT seeds. Our results also revealed that the native promoter guided expression of modified EPSPS genes did not significantly improve the glyphosate tolerance. The present study describing the introduction of a crop-specific TIPS mutation in class I aroA gene of rice and its overexpression have potential to substantially improve the yield and field level glyphosate tolerance in rice. This is the first report to observe that the EPSPS has role to play in improving grain yield of rice.
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Claims 5
Claim · model-written summary
Overexpression of TIPS-OsEPSPS under the ZmUbi promoter raised glyphosate tolerance in rice to up to threefold the recommended dose.
- Taxon
- rice
Oryza sativat0 - Treatment
- overexpression of TIPS-OsEPSPS under the control of the ZmUbi promotere1
- Gene
- TIPS-OsEPSPSe1
- Outcome
- tolerance to glyphosatee1
- Direction
- highere1
- Value
- up to threefold of the recommended dosee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice”
e1“The overexpression of TIPS-OsEPSPS under the control of the ZmUbi promoter resulted in higher tolerance to glyphosate (up to threefold of the recommended dose) without affecting the fitness and related agronomic traits of plants in both controlled and field conditions”
Claim · model-written summary
Overexpression of TIPS-OsEPSPS under the ZmUbi promoter did not affect fitness or related agronomic traits of rice plants in controlled and field conditions.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of TIPS-OsEPSPS under the control of the ZmUbi promotere1
- Gene
- TIPS-OsEPSPSe1
- Outcome
- fitness and related agronomic traitse1
- Direction
- without affectinge1
- Setting
- controlled and field conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice”
e1“The overexpression of TIPS-OsEPSPS under the control of the ZmUbi promoter resulted in higher tolerance to glyphosate (up to threefold of the recommended dose) without affecting the fitness and related agronomic traits of plants in both controlled and field conditions”
Claim · model-written summary
Transgenic rice lines produced 17%-19% more grains than wild type without glyphosate application.
- Taxon
- riceOryza sativat0
- Treatment
- such rice linese2
- Outcome
- grainse2
- Direction
- moree2
- Value
- 17%-19%e2
- Qualifier
- in the absence of glyphosate applicatione2
- Comparator
- wild type (WT)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice”
e2“Furthermore, such rice lines produced 17%-19% more grains compared to the wild type (WT) in the absence of glyphosate application”
Claim · model-written summary
Transgenic rice seeds had significantly higher phenylalanine and tryptophan contents than wild-type seeds.
- Taxon
- riceOryza sativat0
- Organ / stage
- seedse3
- Treatment
- transgenice3
- Outcome
- phenylalanine and tryptophan contentse3
- Direction
- highere3
- Comparator
- WT seedse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice”
e3“the phenylalanine and tryptophan contents in the transgenic seeds were found to be significantly higher in comparison with WT seeds”
Claim · model-written summary
The native promoter-guided expression of modified EPSPS genes did not significantly improve glyphosate tolerance in rice.
- Taxon
- riceOryza sativat0
- Treatment
- native promoter guided expression of modified EPSPS genese4
- Gene
- modified EPSPS genese4
- Outcome
- glyphosate tolerancee4
- Direction
- did not significantly improvee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice”
e4“Our results also revealed that the native promoter guided expression of modified EPSPS genes did not significantly improve the glyphosate tolerance”
The same questions in other papers
5-O-(1-carboxylatovinyl)-3-phosphonatoshikimate · disease · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
transgenic · disease · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 32 in the opposite direction
- positive
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.