Abstract
The color of red-skinned pear (Pyrus spp.) is primarily attributed to accumulation of anthocyanins, which provide nutritional benefits for human health and are closely associated with the commercial value of fruits. Here, we reported the functional characterization of a R2R3-MYB repressor PyMYB107, which forms an 'activator-repressor' loop to control anthocyanin accumulation in the red-skinned pear. PyMYB107 overexpression inhibited anthocyanin biosynthesis in both pear calli and fruits, while virus-induced gene silencing of PyMYB107 increased anthocyanin accumulation in pear fruits. Furthermore, ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato, strawberry and tobacco. PyMYB107 can competitively bind to PybHLH3 with PyMYB10/MYB114, thereby suppressing the transcriptional activation of key anthocyanin biosynthesis genes, PyANS and PyUFGT. Site-directed mutagenesis showed that mutations within the R3 domain and EAR motif of PyMYB107 eliminated its repressive activity. Additionally, PyMYB107 exhibited a comparable expression pattern to PyMYB10/MYB114 and was transcriptionally activated by them. Our finding advanced comprehension of the repression mechanism underlying anthocyanin accumulation, providing valuable molecular insights into improving quality of pear fruits.
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Claims 9
Claim · model-written summary
PyMYB107 overexpression inhibited anthocyanin biosynthesis in pear calli and fruits.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise1 - Organ / stage
- calli and fruitse1
- Treatment
- PyMYB107 overexpressione1
- Gene
- PyMYB107e1
- Outcome
- anthocyanin biosynthesise1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e1“PyMYB107 overexpression inhibited anthocyanin biosynthesis in both pear calli and fruits”
t0“red-skinned pear”
Claim · model-written summary
Virus-induced silencing of PyMYB107 increased anthocyanin accumulation in pear fruits.
- Taxon
- pearPyrus communise2
- Organ / stage
- pear fruitse2
- Treatment
- virus-induced gene silencing of PyMYB107e2
- Gene
- PyMYB107e2
- Outcome
- anthocyanin accumulatione2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“virus-induced gene silencing of PyMYB107 increased anthocyanin accumulation in pear fruits”
t0“red-skinned pear”
Claim · model-written summary
Ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato.
- Taxon
- tomato
Solanum lycopersicume3 - Treatment
- ectopic expression of PyMYB107e3
- Gene
- PyMYB107e3
- Outcome
- anthocyanin accumulatione3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato, strawberry and tobacco”
t0“red-skinned pear”
Claim · model-written summary
Ectopic expression of PyMYB107 decreased anthocyanin accumulation in strawberry.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae3 - Treatment
- ectopic expression of PyMYB107e3
- Gene
- PyMYB107e3
- Outcome
- anthocyanin accumulatione3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e3“ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato, strawberry and tobacco”
t0“red-skinned pear”
Claim · model-written summary
Ectopic expression of PyMYB107 decreased anthocyanin accumulation in tobacco.
- Taxon
- tobacco
Nicotiana tabacume3 - Treatment
- ectopic expression of PyMYB107e3
- Gene
- PyMYB107e3
- Outcome
- anthocyanin accumulatione3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato, strawberry and tobacco”
t0“red-skinned pear”
Claim · model-written summary
PyMYB107 competitively binds PybHLH3 with PyMYB10/MYB114 and thereby suppresses transcriptional activation of PyANS and PyUFGT.
- Taxon
- pearPyrus communist0
- Gene
- PybHLH3e4
- Outcome
- transcriptional activation of key anthocyanin biosynthesis genese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“PyMYB107 can competitively bind to PybHLH3 with PyMYB10/MYB114, thereby suppressing the transcriptional activation of key anthocyanin biosynthesis genes, PyANS and PyUFGT”
t0“red-skinned pear”
Claim · model-written summary
Mutations in the R3 domain and EAR motif of PyMYB107 eliminated its repressive activity.
- Taxon
- pearPyrus communist0
- Treatment
- mutations within the R3 domain and EAR motif of PyMYB107e5
- Gene
- PyMYB107e5
- Outcome
- repressive activitye5
- Direction
- eliminatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Site-directed mutagenesis showed that mutations within the R3 domain and EAR motif of PyMYB107 eliminated its repressive activity”
t0“red-skinned pear”
Claim · model-written summary
PyMYB107 expression pattern is comparable to that of PyMYB10/MYB114.
- Taxon
- pearPyrus communist0
- Gene
- PyMYB107e6
- Outcome
- expression patterne6
- Comparator
- PyMYB10/MYB114e6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“PyMYB107 exhibited a comparable expression pattern to PyMYB10/MYB114 and was transcriptionally activated by them”
t0“red-skinned pear”
Claim · model-written summary
PyMYB107 is transcriptionally activated by PyMYB10/MYB114.
- Taxon
- pearPyrus communist0
- Gene
- PyMYB107e6
- Outcome
- transcriptionally activatede6
- Direction
- activatede6
- Comparator
- PyMYB10/MYB114e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“PyMYB107 exhibited a comparable expression pattern to PyMYB10/MYB114 and was transcriptionally activated by them”
t0“red-skinned pear”
The same questions in other papers
overexpression · quality: phytochemicals · Fragaria x ananassa (strawberry)Positive and negative results
1 in the opposite direction
- positive
overexpression · quality: phytochemicals · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
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overexpression · quality: phytochemicals · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
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1 paper more in this group
See all 5 papers →loss of function · quality: phytochemicals · Pyrus communis (pear)Positive and negative results
1 paper in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.