Abstract
Anthocyanin accumulation in vacuoles results in red coloration in pear peels. Glutathione S-transferase (GST) proteins have emerged as important regulators of anthocyanin accumulation. Here, a total of 57 PcGST genes were identified in the European pear ‘Bartlett’ (Pyrus communis) through comprehensive genomic analysis. Phylogenetic analysis showed that PcGST genes were divided into 10 subfamilies. The gene structure, chromosomal localization, collinearity relationship, cis-elements in the promoter region, and conserved motifs of PcGST genes were analyzed. Further research indicated that glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels. RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which PcGST57 was most significantly induced. Further phylogenetic analysis indicated that PcGST57 was closely related to GST genes identified in other species, which were involved in anthocyanin accumulation. Transcript analysis indicated that PcGST57 was expressed in various tissues, other than flesh, and associated with peel coloration at different developmental stages. Silencing of PcGST57 by virus-induced gene silencing (VIGS) inhibited the expression of PcGST57 and reduced the anthocyanin content in pear fruit. In contrast, overexpression of PcGST57 improved anthocyanin accumulation. Collectively, our results demonstrated that PcGST57 was involved in anthocyanin accumulation in pear and provided candidate genes for red pear breeding.
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Claims 13
Claim · model-written summary
Genome analysis identified 57 PcGST genes in European pear.
Not reportedDirection
Verbatim
e1“a total of 57 PcGST genes were identified in the European pear”
Claim · model-written summary
PcGST genes were divided into 10 subfamilies in pear.
- Taxon
- Pear (Pyrus communis)
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Gene
- PcGST genese1
- Outcome
- subfamiliese1
- Value
- 10e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e1“PcGST genes were divided into 10 subfamilies”
t0“Pear (Pyrus communis)”
Claim · model-written summary
Glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels.
- Taxon
- pearPyrus communise1
- Organ / stage
- peelse1
- Treatment
- glutamic acid (Glu)e1
- Outcome
- anthocyanin accumulatione1
- Direction
- improvee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels”
t0“Pear (Pyrus communis)”
Claim · model-written summary
Glu induced the expression of most PcGST genes in pear.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Treatment
- Glue1
- Gene
- PcGST genese1
- Outcome
- expressione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which PcGST57 was most significantly induced”
t0“Pear (Pyrus communis)”
Claim · model-written summary
PcGST57 was the most significantly induced PcGST gene by Glu in pear.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Treatment
- Glue1
- Gene
- PcGST57e1
- Outcome
- expressione1
- Direction
- most significantly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which PcGST57 was most significantly induced”
t0“Pear (Pyrus communis)”
Claim · model-written summary
PcGST57 is closely related to GST genes of other species in phylogenetic analysis.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Gene
- PcGST57e1
- Outcome
- closely relatede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PcGST57 was closely related to GST genes identified in other species”
t0“Pear (Pyrus communis)”
Claim · model-written summary
PcGST57 was expressed in various pear tissues other than flesh.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Organ / stage
- various tissues, other than fleshe1
- Gene
- PcGST57e1
- Outcome
- expressede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PcGST57 was expressed in various tissues, other than flesh”
t0“Pear (Pyrus communis)”
Claim · model-written summary
PcGST57 expression is associated with peel coloration at different developmental stages in pear.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Organ / stage
- peele1
- Gene
- PcGST57e1
- Outcome
- peel coloratione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PcGST57 was expressed in various tissues, other than flesh, and associated with peel coloration at different developmental stages”
t0“Pear (Pyrus communis)”
Claim · model-written summary
Silencing of PcGST57 by VIGS inhibited PcGST57 expression in pear fruit.
- Taxon
- pearPyrus communise1
- Treatment
- Silencing of PcGST57 by virus-induced gene silencing (VIGS)e1
- Gene
- PcGST57e1
- Outcome
- expressione1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Silencing of PcGST57 by virus-induced gene silencing (VIGS) inhibited the expression of PcGST57 and reduced the anthocyanin content in pear fruit”
t0“Pear (Pyrus communis)”
Claim · model-written summary
Silencing of PcGST57 by VIGS reduced anthocyanin content in pear fruit.
- Taxon
- pearPyrus communise1
- Treatment
- Silencing of PcGST57 by virus-induced gene silencing (VIGS)e1
- Gene
- PcGST57e1
- Outcome
- anthocyanin contente1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Silencing of PcGST57 by virus-induced gene silencing (VIGS) inhibited the expression of PcGST57 and reduced the anthocyanin content in pear fruit”
t0“Pear (Pyrus communis)”
Claim · model-written summary
Overexpression of PcGST57 improved anthocyanin accumulation in pear.
- Taxon
- Pear (Pyrus communis)Pyrus communist0
- Treatment
- overexpression of PcGST57e1
- Gene
- PcGST57e1
- Outcome
- anthocyanin accumulatione1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In contrast, overexpression of PcGST57 improved anthocyanin accumulation”
t0“Pear (Pyrus communis)”
Claim · model-written summary
PcGST57 is involved in anthocyanin accumulation in pear.
- Taxon
- pearPyrus communise1
- Gene
- PcGST57e1
- Outcome
- anthocyanin accumulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Collectively, our results demonstrated that PcGST57 was involved in anthocyanin accumulation in pear”
t0“Pear (Pyrus communis)”
Claim · model-written summary
The study provides candidate genes for red pear breeding.
- Taxon
- pearPyrus communise1
- Outcome
- candidate genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“provided candidate genes for red pear breeding”
t0“Pear (Pyrus communis)”
The same questions in other papers
loss of function · quality: phytochemicals · Pyrus communis (pear)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.