Abstract
Anthocyanins, flavonoids with strong antioxidant activity, give rise to a fruit peel color and provide health benefits. Studies have revealed that melatonin promotes anthocyanin accumulation. However, the function of endogenous hormones in this process remains elusive. Here, we investigated the effect of ethylene signaling on anthocyanin accumulation in melatonin-treated pear fruit. Melatonin promoted anthocyanin accumulation while repressing ethylene production; its effect on anthocyanin biosynthesis in the background of ethephon pretreatment was eliminated. Transcriptome analysis revealed PuERF27, a melatonin-induced ethylene response factor (ERF), as a candidate gene. Transient overexpression of PuERF27 is essential for enhancing anthocyanin accumulation and activating anthocyanin biosynthesis genes, whereas PuERF27 silencing repressed melatonin-induced anthocyanin accumulation. Yeast one-hybrid, electrophoretic mobility shift, and GUS assays indicated that PuERF27 could bind directly to and positively regulate the PuMYB10 and PuGSTF12 promoters. Further studies revealed that PuERF27, together with PuMYB10, synergistically transactivates the PuGSTF12 promoter. These results indicate that melatonin-induced PuERF27 may directly promote PuGSTF12 expression and indirectly activate structural genes through PuMYB10, thereby leading to the biosynthesis and transport of anthocyanins.
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Claims 10
Claim · model-written summary
Melatonin promoted anthocyanin accumulation.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Treatment
- Melatonine0
- Outcome
- anthocyanin accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“Melatonin promoted anthocyanin accumulation while repressing ethylene production”
t0“Pear Fruits”
Claim · model-written summary
Melatonin repressed ethylene production.
- Taxon
- pearPyrus communist0
- Treatment
- Melatonine0
- Outcome
- ethylene productione0
- Direction
- repressinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Melatonin promoted anthocyanin accumulation while repressing ethylene production”
t0“Pear Fruits”
Claim · model-written summary
Ethephon pretreatment eliminated the melatonin effect on anthocyanin biosynthesis.
- Taxon
- pearPyrus communist0
- Treatment
- ethephon pretreatmente0
- Outcome
- anthocyanin biosynthesise0
- Direction
- eliminatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its effect on anthocyanin biosynthesis in the background of ethephon pretreatment was eliminated”
t0“Pear Fruits”
Claim · model-written summary
Transcriptome analysis identified PuERF27 as a melatonin-induced candidate.
- Taxon
- pearPyrus communist0
- Gene
- PuERF27e0
- Outcome
- Transcriptome analysis revealed PuERF27, a melatonin-induced ethylene response factor (ERF), as a candidate genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed PuERF27, a melatonin-induced ethylene response factor (ERF), as a candidate gene”
t0“Pear Fruits”
Claim · model-written summary
PuERF27 overexpression enhanced anthocyanin accumulation.
- Taxon
- pearPyrus communist0
- Treatment
- Transient overexpression of PuERF27e0
- Gene
- PuERF27e0
- Outcome
- anthocyanin accumulatione0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of PuERF27 is essential for enhancing anthocyanin accumulation and activating anthocyanin biosynthesis genes”
t0“Pear Fruits”
Claim · model-written summary
PuERF27 overexpression activated anthocyanin biosynthesis genes.
- Taxon
- pearPyrus communist0
- Treatment
- Transient overexpression of PuERF27e0
- Gene
- PuERF27e0
- Outcome
- anthocyanin biosynthesis genese0
- Direction
- activatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of PuERF27 is essential for enhancing anthocyanin accumulation and activating anthocyanin biosynthesis genes”
t0“Pear Fruits”
Claim · model-written summary
PuERF27 silencing repressed melatonin-induced anthocyanin accumulation.
- Taxon
- pearPyrus communist0
- Treatment
- PuERF27 silencinge0
- Outcome
- melatonin-induced anthocyanin accumulatione0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PuERF27 silencing repressed melatonin-induced anthocyanin accumulation”
t0“Pear Fruits”
Claim · model-written summary
PuERF27 bound and positively regulated the PuMYB10 and PuGSTF12 promoters.
- Taxon
- pearPyrus communist0
- Gene
- PuERF27e0
- Outcome
- PuMYB10 and PuGSTF12 promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PuERF27 could bind directly to and positively regulate the PuMYB10 and PuGSTF12 promoters”
t0“Pear Fruits”
Claim · model-written summary
PuERF27 and PuMYB10 synergistically transactivated the PuGSTF12 promoter.
- Taxon
- pearPyrus communist0
- Gene
- PuERF27e0
- Outcome
- PuGSTF12 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PuERF27, together with PuMYB10, synergistically transactivates the PuGSTF12 promoter”
t0“Pear Fruits”
Claim · model-written summary
The authors infer that PuERF27 promotes anthocyanin biosynthesis and transport through PuGSTF12 and PuMYB10.
- Taxon
- pearPyrus communist0
- Gene
- PuERF27e0
- Outcome
- biosynthesis and transport of anthocyaninse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin-induced PuERF27 may directly promote PuGSTF12 expression and indirectly activate structural genes through PuMYB10, thereby leading to the biosynthesis and transport of anthocyanins”
t0“Pear Fruits”
The same questions in other papers
melatonin · quality: phytochemicals · Pyrus communis (pear)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
loss of function · quality: phytochemicals · Pyrus communis (pear)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.