Abstract
BACKGROUND: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum. RESULT: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants. CONCLUSIONS: Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.
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Claims 6
Claim · model-written summary
EsMYB90 from Eutrema salsugineum was identified as an anthocyanin biosynthesis regulator.
- Taxon
- Eutrema salsugineumEutrema salsugineume0
- Gene
- EsMYB90e0
- Outcome
- anthocyanin biosynthesis regulatore0
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
Claim · model-written summary
EsMYB90 is an R2R3 type of MYB transcription factor.
- Taxon
- Eutrema salsugineumEutrema salsugineume0
- Gene
- EsMYB90e1
- Outcome
- R2R3 type of MYB transcription factore1
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
e1“Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor”
Claim · model-written summary
Ectopic expression of EsMYB90 enhanced pigmentation and anthocyanin accumulation in various organs of tobacco and Arabidopsis.
- Taxon
- tobacco and Arabidopsis
Arabidopsis
Nicotiana tabacume2 - Organ / stage
- various organse2
- Treatment
- Ectopic expression of EsMYB90e2
- Gene
- EsMYB90e2
- Outcome
- pigmentation and anthocyanin accumulatione2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
e2“Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs”
Claim · model-written summary
The transcriptome of 35S:EsMYB90 tobacco showed 42 genes upregulated by EsMYB90 that are required for anthocyanin biosynthesis.
- Taxon
- tobaccoNicotiana tabacume3
- Treatment
- 35S:EsMYB90e3
- Gene
- EsMYB90e3
- Outcome
- 42 genese3
- Direction
- upregulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
e3“42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis”
Claim · model-written summary
EsMYB90 promoted expression of the early anthocyanin biosynthesis genes PAL, CHS and CHI in stems, leaves and flowers of 35S:EsMYB90 tobacco.
- Taxon
- tobaccoNicotiana tabacume4
- Organ / stage
- stems, leaves, and flowerse4
- Treatment
- 35S:EsMYB90e4
- Gene
- PAL, CHS, and CHIe4
- Outcome
- expressione4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
e4“EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants”
Claim · model-written summary
EsMYB90 promoted expression of the late anthocyanin biosynthesis genes DFR, ANS and UFGT in stems, leaves and flowers of 35S:EsMYB90 tobacco.
- Taxon
- tobaccoNicotiana tabacume4
- Organ / stage
- stems, leaves, and flowerse4
- Treatment
- 35S:EsMYB90e4
- Gene
- DFR, ANS, and UFGTe4
- Outcome
- expressione4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum”
e4“EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants”
The same questions in other papers
overexpression · quality: phytochemicals · Nicotiana tabacum (tobacco)Positive and negative results
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See all 5 papers →overexpression · quality: phytochemicals · ArabidopsisPositive and negative results
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.