Abstract
Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here, we report a U-box type E3 ubiquitin ligase PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa. PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo. The expression of GT1 was up-regulated in PUB23-silenced plants, indicating that interacting with PUB23 may directly or indirectly suppress GT1 expression. The silencing of PUB23 led to enhanced immune responses of PAMP-triggered immunity (PTI), including a higher expression level of defense marker genes PR1 and RIN4, and increased accumulation of hydrogen peroxide and superoxide anion. Our results reveal a negative role PUB23 plays in kiwifruit immune responses against Psa and may regulate gene expression by interacting with GT1.
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Claims 8
Claim · model-written summary
PUB23 negatively regulates immune responses against Psa.
- Taxon
- kiwifruit
Actinidia deliciosae0 - Interactor
- Pseudomonas syringae pv. actinidiae
Pseudomonas syringae pv. actinidiaee1pathogen - Gene
- PUB23e0
- Outcome
- immune responses against Psae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa”
e1“Pseudomonas syringae pv. actinidiae (Psa)”
Claim · model-written summary
PUB23 physically interacted with GT1 in vitro and in vivo.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- PUB23e0
- Outcome
- physically interact with GT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo.”
t0“Kiwifruit”
Claim · model-written summary
GT1 expression rose in PUB23-silenced plants.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- PUB23-silencede0
- Gene
- GT1e0
- Outcome
- expression of GT1e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of GT1 was up-regulated in PUB23-silenced plants”
t0“Kiwifruit”
Claim · model-written summary
PUB23 interaction may suppress GT1 expression.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- PUB23e0
- Outcome
- GT1 expressione0
- Qualifier
- may directly or indirectlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interacting with PUB23 may directly or indirectly suppress GT1 expression”
t0“Kiwifruit”
Claim · model-written summary
PUB23 silencing enhanced PAMP-triggered immune responses.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- silencing of PUB23e0
- Gene
- PUB23e0
- Outcome
- immune responses of PAMP-triggered immunity (PTI)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of PUB23 led to enhanced immune responses of PAMP-triggered immunity (PTI)”
t0“Kiwifruit”
Claim · model-written summary
PUB23 silencing raised PR1 and RIN4 expression.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- silencing of PUB23e1
- Gene
- PR1 and RIN4e0
- Outcome
- expression levele0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher expression level of defense marker genes PR1 and RIN4”
e1“The silencing of PUB23 led to enhanced immune responses of PAMP-triggered immunity (PTI)”
t0“Kiwifruit”
Claim · model-written summary
PUB23 silencing increased hydrogen peroxide and superoxide anion accumulation.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- silencing of PUB23e1
- Outcome
- accumulation of hydrogen peroxide and superoxide anione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased accumulation of hydrogen peroxide and superoxide anion”
e1“The silencing of PUB23 led to enhanced immune responses of PAMP-triggered immunity (PTI)”
t0“Kiwifruit”
Claim · model-written summary
PUB23 may regulate gene expression through GT1 interaction.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- GT1e0
- Outcome
- gene expressione0
- Qualifier
- interacting with GT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“may regulate gene expression by interacting with GT1”
t0“Kiwifruit”
The same questions in other papers
loss of function · molecular: expression · Actinidia deliciosa (kiwifruit)Positive and negative results
1 in the opposite direction
- negative
loss of function · physiology: oxidative · Actinidia deliciosa (kiwifruit)Positive and negative results
2 in the opposite direction
- negative
- negative
loss of function · disease · Actinidia deliciosa (kiwifruit)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.