Abstract
The kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) seriously threatens the development of kiwifruit industry. So far, only a limited number of Psa-resistant kiwifruit varieties have been identified, and the underlying molecular mechanisms are still largely unknown. In this study, we evaluated the Psa resistance of six hybrid populations and screened a resistant segregation population R 1 F 2 . Then, transcriptome analysis on the Psa extremely high-resistant (HR) and extremely high-susceptible (HS) plants of the R 1 F 2 population was performed. KEGG enrichment analysis revealed that differentially expressed genes (DEGs) were significantly enriched in plant hormone signal transduction pathways, including auxin, abscisic acid, zeatin, jasmonic acid and salicylic acid. Furthermore, several transcription factors (TFs), especially WRKY TFs, were identified among the DEGs. The qRT-PCR showed that AcWRKY75 was highly expressed in the HS plants. Additionally, AcWRKY75 was significantly induced in the HS cultivar ‘Hongyang’ after Psa inoculation. Sequence amplification analysis showed that there was polymorphism in the DNA sequence of AcWRKY75 gene, but no HR or HS-specific differences were observed. Subcellular localization and transcriptional activity analysis confirmed that AcWRKY75 functions as a nucleus-located transcriptional activator. Transient overexpression of AcWRKY75 in kiwifruit leaves reduced the resistance to Psa, while silencing AcWRKY75 by virus-induced gene silencing (VIGS) slightly enhanced the resistance to Psa. Furthermore, AcWRKY75 exhibited a weak interaction with the promoter of the ABA-related DEG AcBet V1 (Acc27163). Our findings elucidated that AcWRKY75 may negatively regulate the Psa resistance of kiwifruit through the hormone signaling pathway, which laid a foundation for the analysis of the disease resistance mechanism of kiwifruit canker.
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Claims 11
Claim · model-written summary
A Psa-resistant segregating population was screened from six hybrid populations.
Not reportedTaxon
Verbatim
e0“we evaluated the Psa resistance of six hybrid populations and screened a resistant segregation population R 1 F 2”
Claim · model-written summary
Differentially expressed genes were enriched in plant hormone signaling pathways.
- Taxon
- kiwifruit
Actinidia deliciosat0 - Gene
- DEGse0
- Outcome
- plant hormone signal transduction pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs) were significantly enriched in plant hormone signal transduction pathways, including auxin, abscisic acid, zeatin, jasmonic acid and salicylic acid”
t0“kiwifruit”
Claim · model-written summary
Several transcription factors, particularly WRKY factors, were among the differentially expressed genes.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- WRKY TFse0
- Outcome
- WRKY TFse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several transcription factors (TFs), especially WRKY TFs, were identified among the DEGs”
t0“kiwifruit”
Claim · model-written summary
AcWRKY75 was highly expressed in highly susceptible plants.
- Taxon
- kiwifruitActinidia deliciosat0
- Material
- HS plantse0
- Gene
- AcWRKY75e0
- Outcome
- AcWRKY75 was highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcWRKY75 was highly expressed in the HS plants”
t0“kiwifruit”
Claim · model-written summary
Psa inoculation significantly induced AcWRKY75 in susceptible Hongyang.
- Taxon
- kiwifruitActinidia deliciosat0
- Material
- ‘Hongyang’e0
- Treatment
- Psa inoculatione0
- Interactor
- Psae0pathogen
- Gene
- AcWRKY75e0
- Outcome
- AcWRKY75e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcWRKY75 was significantly induced in the HS cultivar ‘Hongyang’ after Psa inoculation”
t0“kiwifruit”
Claim · model-written summary
AcWRKY75 had DNA polymorphism but no resistance-class-specific sequence differences.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcWRKY75e0
- Outcome
- polymorphism in the DNA sequence of AcWRKY75 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was polymorphism in the DNA sequence of AcWRKY75 gene, but no HR or HS-specific differences were observed”
t0“kiwifruit”
Claim · model-written summary
AcWRKY75 was a nucleus-located transcriptional activator.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcWRKY75e0
- Outcome
- nucleus-located transcriptional activatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcWRKY75 functions as a nucleus-located transcriptional activator”
t0“kiwifruit”
Claim · model-written summary
Transient AcWRKY75 overexpression reduced kiwifruit resistance to Psa.
- Taxon
- kiwifruitActinidia deliciosae0
- Organ / stage
- leavese0
- Treatment
- Transient overexpression of AcWRKY75e0
- Interactor
- Psae0pathogen
- Gene
- AcWRKY75e0
- Outcome
- resistance to Psae0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of AcWRKY75 in kiwifruit leaves reduced the resistance to Psa”
Claim · model-written summary
Silencing AcWRKY75 slightly enhanced Psa resistance.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- silencing AcWRKY75 by virus-induced gene silencing (VIGS)e0
- Interactor
- Psae0pathogen
- Gene
- AcWRKY75e0
- Outcome
- resistance to Psae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing AcWRKY75 by virus-induced gene silencing (VIGS) slightly enhanced the resistance to Psa”
t0“kiwifruit”
Claim · model-written summary
AcWRKY75 weakly interacted with the AcBet V1 promoter.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcWRKY75e0
- Outcome
- weak interaction with the promoter of the ABA-related DEG AcBet V1 (Acc27163)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcWRKY75 exhibited a weak interaction with the promoter of the ABA-related DEG AcBet V1 (Acc27163)”
t0“kiwifruit”
Claim · model-written summary
Authors propose that AcWRKY75 negatively regulates Psa resistance through hormone signaling.
- Taxon
- kiwifruitActinidia deliciosae0
- Interactor
- Psae0pathogen
- Gene
- AcWRKY75e0
- Outcome
- Psa resistancee0
- Qualifier
- hormone signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcWRKY75 may negatively regulate the Psa resistance of kiwifruit through the hormone signaling pathway”
The same questions in other papers
loss of function · disease · Actinidia deliciosa (kiwifruit)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
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- negative
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