Paper · 2023 · Plant Stress

RL-WG26 mediated salt stress tolerance in rice seedlings: A new insight into molecular mechanisms

Lei Ren; Yi Zhang; John L. Zhou; et al.

10
claims
18
verbatim quotes
11
citations
4
research areas
✓
Open access · cc-by-nc-nd

Abstract

Soil salinization has significant deleterious impacts on arable lands and crop productivity worldwide. Plant growth–promoting rhizobacteria (PGPR) have the capability to establish mutualistic associations with plants, resulting in the increase of plant growth under abiotic stress. PGPR strain RL-WG26 was previously isolated from the rhizosphere of rice Sea Rice 86 (SR86). In this study, by genome-based average nucleotide identity (ANI) calculation, RL-WG26 was re-identified as Pseudomonas promysalinigenes here instead of Pseudomonas putida. SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation. Ion content analysis demonstrated that seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation. In addition, SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation. At last, genomic analysis of strain RL-WG26 identified several genes and gene clusters encoding 1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase, the tryptophan-dependent indole-3-acetic acid (IAA) synthesis pathway, and the synthesis of betaine. Taken together, PGPR strain RL-WG26 could enhance the growth and salt stress tolerance in SR86 seedlings through various pathways, which provide a foundation for deciphering the molecular mechanisms of strain RL-WG26-mediated plant salt tolerance and promote the application of this strain.

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Claims 10

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Genome analysis reidentified RL-WG26 as Pseudomonas promysalinigenes.

Taxon
Pseudomonas promysalinigenesPseudomonas promysalinigenese0
Outcome
re-identified as Pseudomonas promysalinigenese0
Comparator
Pseudomonas putidae0

Verbatim

e0

“RL-WG26 was re-identified as Pseudomonas promysalinigenes here instead of Pseudomonas putida.”

✓ located verbatim in the source · abstract, characters 453–546 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

RL-WG26 inoculation increased rice seedling fresh and dry biomass under normal or saline conditions.

Material
SR86e0
Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
plant biomass (fresh weight and dry weight)e0
Direction
increasese0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 547–815 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

RL-WG26 inoculation increased rice seedling root surface area and length under normal or saline conditions.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
root surface area and lengthe0
Direction
increasese0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 547–815 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

RL-WG26 inoculation increased rice seedling chlorophyll content under normal or saline conditions.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
chlorophyll contente0
Direction
increasese0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 547–815 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under salinity, RL-WG26 inoculation lowered Na+ and Cl– accumulation in rice seedlings.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
Na+ and Cl– accumulatione0
Direction
lowere0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 855–1118 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under salinity, RL-WG26 inoculation increased K+ and Ca2+ uptake in rice seedlings.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
K+ and Ca2+ uptakee0
Direction
highere0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 855–1118 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RL-WG26 inoculation alleviated salt ion toxicity in rice seedlings.

Treatment
RL-WG26 inoculatede0
Interactor
RL-WG26e0symbiont
Outcome
salt ion toxice0
Direction
alleviatede0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 984–1118 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RL-WG26 inoculation increased antioxidant activity in SR86 seedlings.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
antioxidant activitye0
Direction
highere0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 1132–1311 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RL-WG26 inoculation increased proline concentration in SR86 seedlings.

Treatment
inoculated with RL-WG26e0
Interactor
RL-WG26e0symbiont
Outcome
concentration of osmoregulation factor prolinee0
Direction
highere0
Comparator
those without RL-WG26 inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation.”

✓ located verbatim in the source · abstract, characters 1132–1311 · show in text ↑
t0

“rice seedlings”

✓ located verbatim in the source · title, characters 42–56 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

RL-WG26 genome analysis identified genes for ACC deaminase, tryptophan-dependent IAA synthesis and betaine synthesis.

Taxon
RL-WG26e0
Gene
genes and gene clusterse0
Outcome
1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase, the tryptophan-dependent indole-3-acetic acid (IAA) synthesis pathway, and the synthesis of betainee0

Verbatim

e0

“genomic analysis of strain RL-WG26 identified several genes and gene clusters encoding 1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase, the tryptophan-dependent indole-3-acetic acid (IAA) synthesis pathway, and the synthesis of betaine.”

✓ located verbatim in the source · abstract, characters 1321–1563 · show in text ↑

inoculation · quality: phytochemicals · Oryza sativa (rice)Positive and negative results

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inoculation · growth · Oryza sativa (rice)Positive and negative results

399 papers: 362 positive, 37 negative or no effect

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inoculation · physiology: ion balance · Oryza sativa (rice)Results agree

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inoculation · physiology: osmolytes · Oryza sativa (rice)Results agree

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.