Abstract
Soil salinization has significant deleterious impacts on arable lands and crop productivity worldwide. Plant growth–promoting rhizobacteria (PGPR) have the capability to establish mutualistic associations with plants, resulting in the increase of plant growth under abiotic stress. PGPR strain RL-WG26 was previously isolated from the rhizosphere of rice Sea Rice 86 (SR86). In this study, by genome-based average nucleotide identity (ANI) calculation, RL-WG26 was re-identified as Pseudomonas promysalinigenes here instead of Pseudomonas putida. SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation. Ion content analysis demonstrated that seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation. In addition, SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation. At last, genomic analysis of strain RL-WG26 identified several genes and gene clusters encoding 1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase, the tryptophan-dependent indole-3-acetic acid (IAA) synthesis pathway, and the synthesis of betaine. Taken together, PGPR strain RL-WG26 could enhance the growth and salt stress tolerance in SR86 seedlings through various pathways, which provide a foundation for deciphering the molecular mechanisms of strain RL-WG26-mediated plant salt tolerance and promote the application of this strain.
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Claims 10
Claim · model-written summary
Genome analysis reidentified RL-WG26 as Pseudomonas promysalinigenes.
- Taxon
- Pseudomonas promysalinigenesPseudomonas promysalinigenese0
- Outcome
- re-identified as Pseudomonas promysalinigenese0
- Comparator
- Pseudomonas putidae0
Verbatim
e0“RL-WG26 was re-identified as Pseudomonas promysalinigenes here instead of Pseudomonas putida.”
Claim · model-written summary
RL-WG26 inoculation increased rice seedling fresh and dry biomass under normal or saline conditions.
- Taxon
- rice
Oryza sativat0
- Material
- SR86e0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- plant biomass (fresh weight and dry weight)e0
- Direction
- increasese0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 inoculation increased rice seedling root surface area and length under normal or saline conditions.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- root surface area and lengthe0
- Direction
- increasese0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 inoculation increased rice seedling chlorophyll content under normal or saline conditions.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- chlorophyll contente0
- Direction
- increasese0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR86 seedlings inoculated with RL-WG26 possessed significant increases in plant biomass (fresh weight and dry weight), root surface area and length, and chlorophyll content under either normal or saline conditions, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
Under salinity, RL-WG26 inoculation lowered Na+ and Cl– accumulation in rice seedlings.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- Na+ and Cl– accumulatione0
- Direction
- lowere0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
Under salinity, RL-WG26 inoculation increased K+ and Ca2+ uptake in rice seedlings.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- K+ and Ca2+ uptakee0
- Direction
- highere0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seedlings inoculated with RL-WG26 contained lower Na+ and Cl– accumulation but higher K+ and Ca2+ uptake under saline condition, which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 inoculation alleviated salt ion toxicity in rice seedlings.
- Taxon
- rice
Oryza sativat0
- Treatment
- RL-WG26 inoculatede0
- Interactor
- RL-WG26e0symbiont
- Outcome
- salt ion toxice0
- Direction
- alleviatede0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which finally alleviated the salt ion toxic in the RL-WG26 inoculated seedlings, when compared with those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 inoculation increased antioxidant activity in SR86 seedlings.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- antioxidant activitye0
- Direction
- highere0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 inoculation increased proline concentration in SR86 seedlings.
- Taxon
- rice
Oryza sativat0
- Treatment
- inoculated with RL-WG26e0
- Interactor
- RL-WG26e0symbiont
- Outcome
- concentration of osmoregulation factor prolinee0
- Direction
- highere0
- Comparator
- those without RL-WG26 inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR86 seedlings inoculated with RL-WG26 also exhibited higher antioxidant activity and higher concentration of osmoregulation factor proline than those without RL-WG26 inoculation.”
t0“rice seedlings”
Claim · model-written summary
RL-WG26 genome analysis identified genes for ACC deaminase, tryptophan-dependent IAA synthesis and betaine synthesis.
Verbatim
e0“genomic analysis of strain RL-WG26 identified several genes and gene clusters encoding 1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase, the tryptophan-dependent indole-3-acetic acid (IAA) synthesis pathway, and the synthesis of betaine.”
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