Abstract
Rice is an essential meal for a large section of the world's population including Bangladesh. BRRI Dhan29 is known as an excellent rice variety in Bangladesh but it is now susceptible to several diseases and pests, which might reduce rice yields. Therefore, it becomes necessary to explore and optimize different approach with the aim of enhancing yield, diseases resistance and overall genetic variability of this important rice variety. Ethyl methanesulphonate (EMS) produces a large number of non-lethal point mutations by alkylates guanine bases and leads to mispairing-alkylated guanine pairs with thiamine instead of cytosine. The LD50 of EMS is frequently used as a general indicator of a substance's acute toxicity and a lower LD50 is indicative of increased toxicity. This study aimed to develop a comprehensive protocol for callus induction, regeneration for the BRRI dhan29 rice and to determine the LD50% of EMS on callus survival for further genetic improvement. Mature embryos were used to induce callus by utilizing different concentrations (0.00, 0.5, 1.00, 1.50, 2.00, 2.50, 3.00 and 3.50 mg/L) of 2,4-Dichlorophenoxyactic acid (2, 4-D), while callus regeneration was studied using varying combine concentrations of 1-naphthaleneacetic acid (NAA: 0, 5, 10 and 15 μg/L) and kinetin (0.00, 1.00, 2.00 and 3.00 ml/L). The optimal concentrations for callus induction was 2.0 mg/L 2, 4-D and a combination of 10 μg/L NAA and 2.0 mg/L kinetin were identified for efficient callus regeneration. The LD50 of EMS on BRRI dhan29 calli was 0.31%, indicating its potential mutagen for creating genetic variations. Regenerated plants were successfully acclimatized at field conditions. These findings might provide valuable insights for tissue culture-based studies and genetic improvement efforts for the BRRI dhan29. Bangladesh J. Nuclear Agric, 37(1): 1-10, 2023
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Claims 5
Claim · model-written summary
The optimal 2,4-D concentration for callus induction in rice BRRI dhan29 was 2.0 mg/L.
- Taxon
- Rice
Oryza sativat0 - Material
- BRRI Dhan29t0
- Treatment
- 2.0 mg/L 2, 4-De0
- Outcome
- callus inductione0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Variety BRRI Dhan29”
e0“The optimal concentrations for callus induction was 2.0 mg/L 2, 4-D”
Claim · model-written summary
A combination of 10 μg/L NAA and 2.0 mg/L kinetin was identified as efficient for callus regeneration in rice BRRI dhan29.
- Taxon
- RiceOryza sativat0
- Material
- BRRI Dhan29t0
- Treatment
- 10 μg/L NAA and 2.0 mg/L kinetine0
- Outcome
- efficient callus regeneratione0
- Direction
- efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Variety BRRI Dhan29”
e0“a combination of 10 μg/L NAA and 2.0 mg/L kinetin were identified for efficient callus regeneration”
Claim · model-written summary
The LD50 of EMS on BRRI dhan29 calli was 0.31%.
- Taxon
- RiceOryza sativat0
- Material
- BRRI dhan29e0
- Organ / stage
- callie0
- Treatment
- EMSe0
- Outcome
- LD50e0
- Value
- 0.31%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Variety BRRI Dhan29”
e0“The LD50 of EMS on BRRI dhan29 calli was 0.31%”
Claim · model-written summary
The authors interpret the EMS LD50 as indicating its potential as a mutagen for creating genetic variations.
- Taxon
- RiceOryza sativat0
- Outcome
- genetic variationse0
- Qualifier
- potential mutagene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Variety BRRI Dhan29”
e0“indicating its potential mutagen for creating genetic variations”
Claim · model-written summary
Regenerated plants were successfully acclimatized under field conditions.
- Taxon
- RiceOryza sativat0
- Organ / stage
- Regenerated plantse0
- Outcome
- successfully acclimatizede0
- Setting
- field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Variety BRRI Dhan29”
e0“Regenerated plants were successfully acclimatized at field conditions”
The same questions in other papers
kinetin · growth · Oryza sativa (rice)Results differ
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- negative
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See all 59 papers →1-naphthaleneacetic acid · growth · Oryza sativa (rice)Results differ
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See all 71 papers →2,4-D · growth · Oryza sativa (rice)Positive and negative results
101 papers in the same direction as this paper · 5 in the opposite direction
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See all 107 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.