Abstract
CO 51 is a recently developed semi dwarf, short duration variety of rice popularly grown in Tamil Nadu. Optimization of tissue culture protocol for the variety was one of the most important steps required; in order to make the variety suitable for genetic transformation and gene editing techniques. Immature embryos of CO 51 have been used as explant for callus induction. The immature embryos were cultured on callus induction media (NB media) containing Naphthalene Acetic Acid (NAA) 1 mg l-1, 6-Benzylaminopurine (6-BA) 1 mg l-1 and various concentrations of 2,4 Dichlorophenoxy acetic acid (2,4-D). NB media supplemented with 2.5 mg l-1of 2,4-D have been found to show the highest callus induction frequency that is 94.4%. The calli showing desired features were transferred from NB media to pre-regeneration media (NB-PR) having 2,4-D (2 mg l-1), 6-BA (1 mg l-1 ), NAA (1 mg l-1) where the calli tuned golden- yellow. These calli were then transferred to regeneration media (RNM media) supplemented with 6-BA (3 mg l-1) along with various concentrations of NAA. RNM media with1 mg l-1 NAA showed the highest frequency of shoot induction that is 72.2% with an average of 12 shoots per callus.
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Claims 3
Claim · model-written summary
On NB medium with 2.5 mg/l 2,4-D, callus induction frequency from immature embryos of rice CO 51 was highest (94.4%) among the 2,4-D concentrations tested.
- Taxon
- rice
Oryza sativae0 - Material
- CO 51e0
- Organ / stage
- Immature embryose1
- Treatment
- 2.5 mg l-1of 2,4-De2
- Outcome
- callus induction frequencye2
- Direction
- higheste2
- Value
- 94.4%e2
- Comparator
- various concentrations of 2,4 Dichlorophenoxy acetic acide3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CO 51 is a recently developed semi dwarf, short duration variety of rice”
e1“Immature embryos of CO 51 have been used as explant for callus induction”
e2“NB media supplemented with 2.5 mg l-1of 2,4-D have been found to show the highest callus induction frequency that is 94.4%”
e3“various concentrations of 2,4 Dichlorophenoxy acetic acid (2,4-D)”
Claim · model-written summary
On regeneration media with 1 mg/l NAA, shoot induction frequency of rice CO 51 calli was highest (72.2%) among the NAA concentrations tested.
- Taxon
- riceOryza sativae0
- Organ / stage
- calluse4
- Treatment
- 1 mg l-1 NAAe4
- Outcome
- frequency of shoot inductione4
- Direction
- higheste4
- Value
- 72.2%e4
- Comparator
- various concentrations of NAAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CO 51 is a recently developed semi dwarf, short duration variety of rice”
e4“RNM media with1 mg l-1 NAA showed the highest frequency of shoot induction that is 72.2% with an average of 12 shoots per callus”
e5“supplemented with 6-BA (3 mg l-1) along with various concentrations of NAA”
Claim · model-written summary
On regeneration media with 1 mg/l NAA, rice CO 51 calli produced on average 12 shoots per callus.
- Taxon
- riceOryza sativae0
- Organ / stage
- calluse4
- Outcome
- shoots per calluse4
- Value
- 12e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CO 51 is a recently developed semi dwarf, short duration variety of rice”
e4“RNM media with1 mg l-1 NAA showed the highest frequency of shoot induction that is 72.2% with an average of 12 shoots per callus”
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See all 107 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.