Abstract
Plant growth regulators were used to test callus induction and in vitro regeneration in six rice genotypes (RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97). Four different concentrations (1, 2, 3 and 4 mg/L) of 2,4-D for callus induction and three different concentrations (1,2 and 3 mg/L) of NAA with three doses (5,10 and 15 µ/L) of kinetin for callus regeneration were used to test the effect of plant growth regulators. This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D. In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively. In the regeneration, it was found that an MS medium enriched with 2 g/L Kinetin and 10 µm/L NAA has the ability to induce increased regeneration of different rice varieties (RM-AC-2 (72.4%), BRRI dhan89 (66.9%), BRRI dhan88 (62.5%), Nipponbare (63.3%), Koshihikari (48%) and Zenshan97 (39.6%). From the regenerated plants, one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g. This rice plant is very promising for high yielding rice variety development program in Bangladesh. The improved callus development and regeneration ability of this genotype might be helpful for future rice variety development and genetic transformation program.
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Claims 10
Claim · model-written summary
MS medium with 2 mg/L 2, 4-D gave high rice callus induction.
- Taxon
- rice
Oryza sativat0 - Treatment
- MS medium enriched with 2 mg/L 2, 4-De0
- Outcome
- callus inductione0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for RM-AC-2 was 92.7%.
- Taxon
- riceOryza sativat0
- Material
- RM-AC-2e0
- Outcome
- callus induction frequenciese0
- Value
- 92.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for BRRI dhan89 was 87.8%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan89e0
- Outcome
- callus induction frequenciese0
- Value
- 87.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for BRRI dhan88 was 84.6%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan88e0
- Outcome
- callus induction frequenciese0
- Value
- 84.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for Nipponbare was 82.9%.
- Taxon
- riceOryza sativat0
- Material
- Nipponbaree0
- Outcome
- callus induction frequenciese0
- Value
- 82.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for Koshihikari was 86.2%.
- Taxon
- riceOryza sativat0
- Material
- Koshihikarie0
- Outcome
- callus induction frequenciese0
- Value
- 86.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
Callus induction frequency for Zenshan97 was 62.9%.
- Taxon
- riceOryza sativat0
- Material
- Zenshan97e0
- Outcome
- callus induction frequenciese0
- Value
- 62.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively.”
t0“Rice Plant”
Claim · model-written summary
One regenerated RM-AC-2 rice plant completed its life cycle.
- Taxon
- riceOryza sativat0
- Material
- RM-AC-2e0
- Outcome
- complete its life cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one plant of the RM-AC-2 genotype availed to complete its life cycle”
t0“Rice Plant”
Claim · model-written summary
That regenerated RM-AC-2 plant generated 32 effective tillers.
- Taxon
- riceOryza sativat0
- Material
- RM-AC-2e0
- Outcome
- effective tillerse0
- Value
- 32 effective tillerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers”
t0“Rice Plant”
Claim · model-written summary
That regenerated RM-AC-2 plant yielded 89g.
- Taxon
- riceOryza sativat0
- Material
- RM-AC-2e0
- Outcome
- yieldede0
- Value
- 89ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g”
t0“Rice Plant”
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See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.