Abstract
A new and rapid protocol for optimum callus production and complete plant regeneration has been assessed in Malaysian upland rice (Oryza sativa) cv. Panderas. The effect of plant growth regulator (PGR) on the regeneration frequency of Malaysian upland rice (cv. Panderas) was investigated. Mature seeds were used as a starting material for callus induction experiment using various concentrations of 2,4-D and NAA. Optimal callus induction frequency at 90% was obtained on MS media containing 2,4-D (3 mg L(-1)) and NAA (2 mg L(-1)) after 6 weeks while no significant difference was seen on tryptophan and glutamine parameters. Embryogenic callus was recorded as compact, globular and light yellowish in color. The embryogenic callus morphology was further confirmed with scanning electron microscopy (SEM) analysis. For regeneration, induced calli were treated with various concentrations of Kin (0.5-1.5 mg L(-1)), BAP, NAA and 0.5 mg L(-1) of TDZ. The result showed that the maximum regeneration frequency (100%) was achieved on MS medium containing BAP (0.5 mg L(-1)), Kin (1.5 mg L(-1)), NAA (0.5 mg L(-1)) and TDZ (0.5 mg L(-1)) within four weeks. Developed shoots were successfully rooted on half strength MS free hormone medium and later transferred into a pot containing soil for acclimatization. This cutting-edge finding is unique over the other existing publishable data due to the good regeneration response by producing a large number of shoots.
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Claims 7
Claim · model-written summary
Optimal callus induction frequency of 90% was obtained on MS media with 2,4-D (3 mg L(-1)) and NAA (2 mg L(-1)) after 6 weeks.
- Taxon
- Oryza sativa
Oryza sativae0 - Material
- cv. Panderase0
- Organ / stage
- Mature seedse2
- Treatment
- 2,4-D (3 mg L(-1)) and NAA (2 mg L(-1))e1
- Outcome
- callus induction frequencye1
- Value
- 90%e1
- Qualifier
- after 6 weekse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e1“Optimal callus induction frequency at 90% was obtained on MS media containing 2,4-D (3 mg L(-1)) and NAA (2 mg L(-1)) after 6 weeks”
e2“Mature seeds were used as a starting material”
Claim · model-written summary
No significant difference was seen on tryptophan and glutamine parameters of callus induction.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- tryptophan and glutaminee1
- Outcome
- parameterse1
- Direction
- no significant differencee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e1“no significant difference was seen on tryptophan and glutamine parameters”
Claim · model-written summary
Embryogenic callus was compact, globular and light yellowish in color.
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- Embryogenic calluse3
- Qualifier
- compact, globular and light yellowish in colore3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e3“Embryogenic callus was recorded as compact, globular and light yellowish in color”
Claim · model-written summary
The embryogenic callus morphology was confirmed with scanning electron microscopy (SEM) analysis.
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- embryogenic callus morphologye4
- Qualifier
- scanning electron microscopy (SEM) analysise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e4“The embryogenic callus morphology was further confirmed with scanning electron microscopy (SEM) analysis”
Claim · model-written summary
The maximum regeneration frequency of 100% was achieved on MS medium with BAP, Kin, NAA and TDZ within four weeks.
- Taxon
- Oryza sativaOryza sativae0
- Organ / stage
- induced callie6
- Treatment
- BAP (0.5 mg L(-1)), Kin (1.5 mg L(-1)), NAA (0.5 mg L(-1)) and TDZ (0.5 mg L(-1))e5
- Outcome
- regeneration frequencye5
- Direction
- maximume5
- Value
- 100%e5
- Qualifier
- within four weekse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e5“maximum regeneration frequency (100%) was achieved on MS medium containing BAP (0.5 mg L(-1)), Kin (1.5 mg L(-1)), NAA (0.5 mg L(-1)) and TDZ (0.5 mg L(-1)) within four weeks”
e6“induced calli were treated”
Claim · model-written summary
Developed shoots were successfully rooted on half strength MS free hormone medium.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- half strength MS free hormone mediume6
- Outcome
- Developed shootse6
- Qualifier
- successfully rootede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e6“Developed shoots were successfully rooted on half strength MS free hormone medium”
Claim · model-written summary
The regeneration response produced a large number of shoots.
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- number of shootse1
- Qualifier
- good regeneration responsee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malaysian upland rice (Oryza sativa) cv. Panderas”
e1“due to the good regeneration response by producing a large number of shoots”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.