Abstract
SUPPRESSOR OF MAX2-LIKE 6, 7, and 8 (SMXL6,7,8) function as repressors and transcription factors of the strigolactone (SL) signaling pathway, playing an important role in the development and stress tolerance in Arabidopsis thaliana. However, the molecular mechanism by which SMXL6,7,8 negatively regulate drought tolerance and ABA response remains largely unexplored. In the present study, the interacting protein and downstream target genes of SMXL6,7,8 were investigated. Our results showed that the substrate receptor for the CUL4-based E3 ligase DDB1-BINDING WD-REPEAT DOMAIN (DWD) HYPERSENSITIVE TO ABA DEFICIENT 1 (ABA1) (DWA1) physically interacted with SMXL6,7,8. The degradation of SMXL6,7,8 proteins were partially dependent on DWA1. Disruption of SMXL6,7,8 resulted in increased drought tolerance and could restore the drought-sensitive phenotype of the dwa1 mutant. In addition, SMXL6,7,8 could directly bind to the promoter of SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2.3 (SnRK2.3) to repress its transcription. The mutations in SnRK2.2/2.3 significantly suppressed the hypersensitivity of smxl6/7/8 to ABA-mediated inhibition of seed germination. Conclusively, SMXL6,7,8 interact with DWA1 to negatively regulate drought tolerance and target ABA-response genes. These data provide insights into drought tolerance and ABA response in Arabidopsis via the SMXL6,7,8-mediated SL signaling pathway.
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Claims 8
Claim · model-written summary
DWA1 physically interacts with SMXL6,7,8.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- DWA1e1
- Outcome
- physically interacted with SMXL6,7,8e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e1“(DWA1) physically interacted with SMXL6,7,8”
Claim · model-written summary
Degradation of SMXL6,7,8 proteins is partially dependent on DWA1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- DWA1e2
- Outcome
- degradation of SMXL6,7,8 proteinse2
- Qualifier
- partially dependente2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e2“The degradation of SMXL6,7,8 proteins were partially dependent on DWA1”
Claim · model-written summary
Disruption of SMXL6,7,8 increased drought tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Disruption of SMXL6,7,8e3
- Outcome
- drought tolerancee3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e3“Disruption of SMXL6,7,8 resulted in increased drought tolerance”
Claim · model-written summary
Disruption of SMXL6,7,8 restored the drought-sensitive phenotype of the dwa1 mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Disruption of SMXL6,7,8e3
- Outcome
- drought-sensitive phenotypee4
- Direction
- restoree4
- Comparator
- dwa1 mutante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e3“Disruption of SMXL6,7,8 resulted in increased drought tolerance”
e4“could restore the drought-sensitive phenotype of the dwa1 mutant”
Claim · model-written summary
SMXL6,7,8 directly binds the SnRK2.3 promoter and represses its transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- SnRK2.3e5
- Outcome
- transcriptione5
- Direction
- represse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e5“SMXL6,7,8 could directly bind to the promoter of SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2.3 (SnRK2.3) to repress its transcription”
Claim · model-written summary
Mutations in SnRK2.2/2.3 significantly suppressed the hypersensitivity of smxl6/7/8 to ABA-mediated inhibition of seed germination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- mutations in SnRK2.2/2.3e6
- Outcome
- hypersensitivity of smxl6/7/8 to ABA-mediated inhibition of seed germinatione6
- Direction
- suppressede6
- Comparator
- smxl6/7/8e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e6“The mutations in SnRK2.2/2.3 significantly suppressed the hypersensitivity of smxl6/7/8 to ABA-mediated inhibition of seed germination”
Claim · model-written summary
SMXL6,7,8 interact with DWA1 to negatively regulate drought tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- DWA1e7
- Outcome
- drought tolerancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e7“SMXL6,7,8 interact with DWA1 to negatively regulate drought tolerance”
Claim · model-written summary
SMXL6,7,8 target ABA-response genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- SMXL6,7,8e7
- Outcome
- ABA-response genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress tolerance in Arabidopsis thaliana”
e7“SMXL6,7,8 interact with DWA1 to negatively regulate drought tolerance”
e1“target ABA-response genes”
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