Abstract
A cDNA expression library from Brassica juncea was introduced into the fission yeast Schizosaccharomyces pombe to select for transformants tolerant to cadmium. Transformants expressing OXIDATIVE STRESS 3 (OXS3) or OXS3-Like cDNA exhibited enhanced tolerance to a range of metals and oxidizing chemicals. OXS3 belongs to a family of proteins that share a highly conserved domain corresponding to a putative N-acetyltransferase or thioltransferase catalytic site. Mutations within this conserved domain abolished the ability of Arabidopsis thaliana OXS3 to enhance stress tolerance in S. pombe, indicating a role in stress tolerance for the presumptive catalytic domain. A stress-sensitive mutant of AtOXS3 and enhanced tolerance of overexpression lines support the role of OXS3 in stress tolerance. The expression of tagged B. juncea and A. thaliana OXS3 proteins in plant cells revealed a subnuclear speckling pattern related to the nucleosome in discrete parts of the chromatin. It is possible that OXS3 might act as a chromatin remodeling factor for the stress response.
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Claims 7
Claim · model-written summary
OXS3 and OXS3-Like expression enhanced yeast tolerance to metals and oxidizing chemicals.
- Taxon
- Brassica juncea
Brassica junceae0 - Treatment
- expressing OXIDATIVE STRESS 3 (OXS3) or OXS3-Like cDNAe0
- Gene
- OXIDATIVE STRESS 3 (OXS3) or OXS3-Likee0
- Outcome
- tolerance to a range of metals and oxidizing chemicalse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA expression library from Brassica juncea was introduced into the fission yeast Schizosaccharomyces pombe to select for transformants tolerant to cadmium. Transformants expressing OXIDATIVE STRESS 3 (OXS3) or OXS3-Like cDNA exhibited enhanced tolerance to a range of metals and oxidizing chemicals.”
Claim · model-written summary
OXS3 proteins share a conserved domain corresponding to a putative catalytic site.
- Taxon
- Brassica junceaBrassica junceae1
- Gene
- OXS3e0
- Outcome
- highly conserved domain corresponding to a putative N-acetyltransferase or thioltransferase catalytic sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OXS3 belongs to a family of proteins that share a highly conserved domain corresponding to a putative N-acetyltransferase or thioltransferase catalytic site.”
e1“A cDNA expression library from Brassica juncea was introduced into the fission yeast Schizosaccharomyces pombe”
Claim · model-written summary
Mutating the conserved Arabidopsis OXS3 domain abolished enhanced yeast stress tolerance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Mutations within this conserved domaine0
- Gene
- OXS3e0
- Outcome
- stress tolerancee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations within this conserved domain abolished the ability of Arabidopsis thaliana OXS3 to enhance stress tolerance in S. pombe”
Claim · model-written summary
An AtOXS3 mutant was stress-sensitive.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- mutant of AtOXS3e0
- Gene
- AtOXS3e0
- Outcome
- stress-sensitivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A stress-sensitive mutant of AtOXS3”
e1“Arabidopsis thaliana OXS3”
Claim · model-written summary
OXS3 overexpression lines had enhanced stress tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpression linese0
- Gene
- OXS3e0
- Outcome
- tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced tolerance of overexpression lines support the role of OXS3 in stress tolerance.”
e1“Arabidopsis thaliana OXS3”
Claim · model-written summary
Tagged OXS3 proteins showed subnuclear speckles related to nucleosomes in chromatin.
- Taxon
- B. junceaBrassica junceae0
- Gene
- OXS3e0
- Outcome
- subnuclear speckling pattern related to the nucleosome in discrete parts of the chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of tagged B. juncea and A. thaliana OXS3 proteins in plant cells revealed a subnuclear speckling pattern related to the nucleosome in discrete parts of the chromatin.”
Claim · model-written summary
OXS3 may act as a chromatin remodeling factor in the stress response.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- OXS3e0
- Outcome
- stress responsee0
- Qualifier
- chromatin remodeling factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is possible that OXS3 might act as a chromatin remodeling factor for the stress response.”
e1“Arabidopsis thaliana OXS3”
The same questions in other papers
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3 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
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11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.