Paper · 2023 · Basrah Journal of Agricultural Sciences

Molecular Identification of Postharvest Moldy Core Pathogens on Apple and Application of Biocontrol Products of Essential Oils (EOs) and Trichoderma harzianum

Asmar A. Yousif; Wazeer A. Hassan

18
claims
36
verbatim quotes
3
citations
3
research areas
✓
Open access · cc-by-nc

Abstract

This study aimed to identify prevalent pathogens of a caused moldy core of postharvest apple fruits and the efficiency of essential oils (EO) of clove (Syzygium aromaticum), eucalyptus (Eucalyptus globulus), sage (Salvia officinalis), and thyme (Thymus vulgaris), and Trichoderma harzianum filtrate to inhibit pathogens growth of Alternaria alternata, Botrytis cinerea, and Penicillium griseofulvum. The examined pathogens are recognized dependent on morphological and also molecular identification. In vivo, clove EO and T. harzianum filtrate were strongly restricted decay area on fruits with 82.36% and 81.69%, respectively when applied as direct inhibition. Growth of all examined pathogens was entirely stopped on fruits treated with both clove and thyme oils at 10%. The results also illustrated that T. harzianum filtrate and EOs exhibited considerable growth inhibition of B. cinerea and ranged between 86.53% and 100%. The lowest inhibitory potential of EOs 47.95% and 75.9% were observed with P. griseofulvum. T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively. The growth of both pathogens A. alternata and B. cinerea depressed with up to 90% using T. harzianum filtrate followed by EOs of eucalyptus and thyme. Whereas fruits inoculated with P. griseofulvum were not frustrated when applied to each EOs or T. harzianum. Their systemic induction was restricted between 3.16% and 23.82%.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 18

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The examined apple moldy-core pathogens were identified morphologically and molecularly.

Outcome
pathogense0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The examined pathogens are recognized dependent on morphological and also molecular identification.”

✓ located verbatim in the source · abstract, characters 400–499 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

clove EO restricted decay area on apple fruits by 82.36%.

Treatment
clove EOe0
Outcome
decay area on fruitse0
Direction
restrictede0
Value
82.36%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“In vivo, clove EO and T. harzianum filtrate were strongly restricted decay area on fruits with 82.36% and 81.69%, respectively when applied as direct inhibition.”

✓ located verbatim in the source · abstract, characters 500–661 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum filtrate restricted decay area on apple fruits by 81.69%.

Treatment
T. harzianum filtratee0
Outcome
decay area on fruitse0
Direction
restrictede0
Value
81.69%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“In vivo, clove EO and T. harzianum filtrate were strongly restricted decay area on fruits with 82.36% and 81.69%, respectively when applied as direct inhibition.”

✓ located verbatim in the source · abstract, characters 500–661 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Clove oil at 10% completely stopped pathogen growth on apple fruits.

Treatment
clovee0
Outcome
Growth of all examined pathogense0
Direction
entirely stoppede0
Value
10%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Growth of all examined pathogens was entirely stopped on fruits treated with both clove and thyme oils at 10%.”

✓ located verbatim in the source · abstract, characters 662–772 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thyme oil at 10% completely stopped pathogen growth on apple fruits.

Treatment
thyme oilse0
Outcome
Growth of all examined pathogense0
Direction
entirely stoppede0
Value
10%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Growth of all examined pathogens was entirely stopped on fruits treated with both clove and thyme oils at 10%.”

✓ located verbatim in the source · abstract, characters 662–772 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum filtrate inhibited B. cinerea growth by 86.53% to 100%.

Treatment
T. harzianum filtratee0
Interactor
B. cinereaBotrytis cinereae0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
growth inhibition of B. cinereae0
Direction
inhibitione0
Value
86.53% and 100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Botrytis cinerea
Botrytis cinerea · InteractorRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The results also illustrated that T. harzianum filtrate and EOs exhibited considerable growth inhibition of B. cinerea and ranged between 86.53% and 100%.”

✓ located verbatim in the source · abstract, characters 773–927 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oils inhibited B. cinerea growth by 86.53% to 100%.

Treatment
EOse0
Interactor
B. cinereaBotrytis cinereae0pathogen
Outcome
growth inhibition of B. cinereae0
Direction
inhibitione0
Value
86.53% and 100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Botrytis cinerea
Botrytis cinerea · InteractorRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The results also illustrated that T. harzianum filtrate and EOs exhibited considerable growth inhibition of B. cinerea and ranged between 86.53% and 100%.”

✓ located verbatim in the source · abstract, characters 773–927 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Essential oils showed their lowest inhibition against P. griseofulvum, at 47.95% and 75.9%.

Interactor
P. griseofulvumPenicillium griseofulvume0pathogen
Outcome
inhibitory potential of EOse0
Direction
loweste0
Value
47.95% and 75.9%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The lowest inhibitory potential of EOs 47.95% and 75.9% were observed with P. griseofulvum.”

✓ located verbatim in the source · abstract, characters 928–1019 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum filtrate inhibited apple fruit decay by 64.5%.

Treatment
T. harzianum filtratee0
Outcome
fruit decaye0
Direction
inhibitede0
Value
64.5%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively.”

✓ located verbatim in the source · abstract, characters 1020–1225 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

thyme inhibited apple fruit decay by 45.9%.

Treatment
thymee0
Outcome
fruit decaye0
Direction
inhibitede0
Value
45.9%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively.”

✓ located verbatim in the source · abstract, characters 1020–1225 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

sage inhibited apple fruit decay by 38.6%.

Treatment
sagee0
Outcome
fruit decaye0
Direction
inhibitede0
Value
38.6%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively.”

✓ located verbatim in the source · abstract, characters 1020–1225 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

eucalyptus inhibited apple fruit decay by 37.5%.

Treatment
eucalyptuse0
Outcome
fruit decaye0
Direction
inhibitede0
Value
37.5%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively.”

✓ located verbatim in the source · abstract, characters 1020–1225 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

clove inhibited apple fruit decay by 35.9%.

Treatment
clovee0
Outcome
fruit decaye0
Direction
inhibitede0
Value
35.9%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum filtrate was the most effective biocontrol that inhibited fruit decay by 64.5% followed by 45.9%, 38.6%, 37.5%, and 35.9% when utilized EOs of thyme, sage, eucalyptus, and clove, respectively.”

✓ located verbatim in the source · abstract, characters 1020–1225 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum filtrate suppressed A. alternata growth by up to 90%.

Treatment
T. harzianum filtratee0
Interactor
A. alternataAlternaria alternatae0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
growth of both pathogens A. alternata and B. cinereae0
Direction
depressede0
Value
up to 90%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Alternaria alternata
Alternaria alternata · Interactor · picture from its Wikimedia Commons categoryMarta Hiromi Taniwaki, Secretaria de Agricultura e Abastecimento · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The growth of both pathogens A. alternata and B. cinerea depressed with up to 90% using T. harzianum filtrate followed by EOs of eucalyptus and thyme.”

✓ located verbatim in the source · abstract, characters 1226–1376 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum filtrate suppressed B. cinerea growth by up to 90%.

Treatment
T. harzianum filtratee0
Interactor
B. cinereaBotrytis cinereae0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
growth of both pathogens A. alternata and B. cinereae0
Direction
depressede0
Value
up to 90%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Botrytis cinerea
Botrytis cinerea · InteractorRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The growth of both pathogens A. alternata and B. cinerea depressed with up to 90% using T. harzianum filtrate followed by EOs of eucalyptus and thyme.”

✓ located verbatim in the source · abstract, characters 1226–1376 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Essential oils did not suppress P. griseofulvum on apple fruits.

Treatment
EOse0
Interactor
P. griseofulvumPenicillium griseofulvume0pathogen
Outcome
fruits inoculated with P. griseofulvume0
Direction
not frustratede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Whereas fruits inoculated with P. griseofulvum were not frustrated when applied to each EOs or T. harzianum.”

✓ located verbatim in the source · abstract, characters 1377–1485 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

T. harzianum did not suppress P. griseofulvum on apple fruits.

Treatment
T. harzianume0
Interactor
P. griseofulvumPenicillium griseofulvume0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
fruits inoculated with P. griseofulvume0
Direction
not frustratede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Whereas fruits inoculated with P. griseofulvum were not frustrated when applied to each EOs or T. harzianum.”

✓ located verbatim in the source · abstract, characters 1377–1485 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Systemic induction was limited to 3.16%–23.82%.

Outcome
systemic inductione0
Direction
restrictede0
Value
3.16% and 23.82%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Their systemic induction was restricted between 3.16% and 23.82%.”

✓ located verbatim in the source · abstract, characters 1486–1551 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 64–69 · show in text ↑

Syzygium aromaticum · growth · Malus domestica (apple)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

Trichoderma harzianum · disease · Malus domestica (apple)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Syzygium aromaticum · disease · Malus domestica (apple)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

botanical · disease · Malus domestica (apple)Positive and negative results

39 papers: 36 positive, 3 negative or no effect

35 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. no effect

35 papers more in this group

See all 39 papers →

botanical · Botrytis cinerea · Malus domestica (apple)Results agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.